Eight myeloma cell lines were used in this study. The RPMI-8226, U-266, MM.1S, and IM-9 MM cells were purchased from American Type Culture Collection (ATCC, USA). Myeloma cell lines KMS-28-BM, KMS-20, KMS-12-BM, and KMS-21-BM were obtained from Japanese Collection of Research Bioresources (JCRB) cell bank. Cells were cultured with RPMI1640 medium (ATCC) supplemented with 10–15% fetal bovine serum (Lonza) in an incubator at 37 °C with humidified 5% CO2. Cells were passaged every 3–4 days.
Kms 21 bm
Manufactured by Japanese Collection of Research Bioresources Cell Bank
Sourced in United States, Japan
The KMS-21-BM is a multi-functional cell incubator designed for cell culture applications. It maintains a stable and controlled environment for cell growth and proliferation. The key functions include temperature, humidity, and CO2 regulation to support optimal cell culture conditions.
Automatically generated - may contain errors
Lab products found in correlation
Kms12bm, by Japanese Collection of Research Bioresources Cell Bank (3 mentions)
Kms 11, by Japanese Collection of Research Bioresources Cell Bank (3 mentions)
Kms 26, by Japanese Collection of Research Bioresources Cell Bank (3 mentions)
Kms 28bm, by Japanese Collection of Research Bioresources Cell Bank (2 mentions)
Kms 20, by Japanese Collection of Research Bioresources Cell Bank (2 mentions)
Kms34, by Japanese Collection of Research Bioresources Cell Bank (2 mentions)
Kms 12 pe, by Japanese Collection of Research Bioresources Cell Bank (2 mentions)
Rpmi8226, by American Type Culture Collection (2 mentions)
Rpmi 1640 medium, by American Type Culture Collection (1 mentions)
Mm 1s, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by Lonza (1 mentions)
Nci h929, by American Type Culture Collection (1 mentions)
Opm 2 acc 50, by Leibniz Institute DSMZ (1 mentions)
Kms18, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Kms27, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Mm 1s cells, by American Type Culture Collection (1 mentions)
Hl 60, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
5 protocols using kms 21 bm
1
Myeloma Cell Line Culture Protocol
2
Multiple Myeloma Cell Lines Protocol
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3
Characterization of Multiple Myeloma Cell Lines
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Fourteen MM cell lines and HS‐5 bone marrow stromal cells (BMSC) were used. EJM (ACC‐560), LP‐1 (ACC‐41) and OPM‐2 (ACC‐50) were purchased from DSMZ. KMS‐11 (JCRB1179) was obtained from the Health Science Research Resources Bank. KMS‐12‐BM (JCRB0429), KMS‐12‐PE (JCRB0430), KMS‐21‐BM (JCRB1185), KMS‐26 (JCRB1187), KMS‐28‐BM (JCRB1192) and KMS‐34 (JCRB1195) were obtained from the Japanese Collection of Research Bioresources Cell Bank. HS‐5 (CRL‐11882), MM.1S (CRL‐2974), MM.1R (CRL‐2975), NCI‐H929 (CRL‐9068) and U266B1 (TIB‐196) were obtained from the American Type Culture Collection. EMJ was cultured in Iscove’s modified Dulbecco’s medium supplemented with 20% FBS. HS‐5 was cultured in DMEM supplemented with 10% FBS. NCI‐H929 was cultured in RPMI 1640 medium supplemented with 10% FBS and 0.05 mmol/L 2‐mercaptoethanol. U266B1 was cultured in RPMI 1640 medium supplemented with 15% FBS. The other cell lines were cultured in RPMI 1640 medium supplemented with 10% FBS. All cell lines used were authenticated by means of short tandem repeat‐based DNA profiling.
4
Cell Line Validation Protocol
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RPMI8226 and MM.1S cells were purchased from ATCC. KMS11, KMS12BM, KMS12PE, KMS18, KMS20, KMS21BM, KMS27, KMS28, and KMS34 were purchased from the Japanese Collection of Research Bioresources Cell Bank. OPM2 was provided by Nizar Bahlis (University of Calgary). All other cell lines were provided by Jonathan Keats. All cell lines were validated by using sequencing and phenotypic characterization.
5
Myeloma and Leukemia Cell Lines Study
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Human myeloma-derived cell lines (HMCLs), KMS-11, KMS-21-BM, KMS-26, and acute myelogenous leukemia-derived cell line HL-60 were purchased from the Japanese Collection of Research Bioresources (Osaka, Japan). BM samples were obtained from patients with MGUS (n = 12), NDMM (n = 23), and RRMM (n = 35) between September 2017 and March 2021 at the Division of Hematology and Oncology, Department of Medicine, Kyoto Prefectural University of Medicine (KPUM). MGUS/MM was diagnosed based on the International Myeloma Working Group 2014 criteria [19 (link)]. Written informed consent was obtained from all patients. The study was conducted in compliance with the Declaration of Helsinki, and the study protocol was approved by the institutional review board of KPUM (ERB-C-930-1).
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