Monoclonal anti cytochrome c antibody

Calcium ionophore (A23187), benzamidine hydrochloride, N-acetyl-Leu-Glu-His-Asp trifluoro methylcoumarin (AC-LEHD-FMC), acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin (AC-DEVD-AMC), sodium orthovanadate, dimethyl sulfoxide (DMSO), fluorescein isothiocyanate (FITC)-labeled annexin V, 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate acetylester (CM-H2DCFDA), CHAPS, rhodamine 123, leupeptin hydrochoride, N-(2-Hydroxyethyl) piperazine-N′-ethanesulfonic acid (HEPES), fura-2/AM, monoclonal anti-phosphotyrosine antibody, acridine orange 10-nonyl bromide (NAO) and dithiothreitol (DTT) were from Sigma Chemicals, St. Louis (USA). Monoclonal anti-cytochrome c antibody and anti-β-actin were from Epitomics Burlingame, CA (USA). Anti-Caspase-3 antibody was from Santa Cruz Biotechnology, Inc. Texas (USA). Collagen type-I was from Chrono-log Corporation, Pennsylvania (USA). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and 1, 1-diphenyl-2-picrylhdrazyl (DPPH) were from HiMedia Laboratories, Mumbai (India). Lactate dehydrogenase (LDH) kit was from AGAPPE diagnostics Ltd., Kerala (India). γ-glutamyl p-nitroanilide and glycylglycine were from Sisco Research laboratories Pvt Ltd., Mumbai (India). All other reagents were of analytical grade.

All fluorogenic, non-fluorogenic dyes, Histopaque-1077 and standard platelet agonists were from Sigma Chemicals, USA. Monoclonal anti-cytochrome c antibody was from Epitomics, USA. z-DEVD-fmk, SB203580, ABT-737, Pifithrin-μ, antibodies against caspase-3, caspase-9, Bax, Bcl-2, BAD, phospho-BAD (ser136), Bid and tBid were from Santa Cruz Biotechnology, Inc., USA. Phospho-p38 (Thr180/Tyr182), p38, phospho-p53 (ser15), p53, COX-IV and β-tubulin antibodies were from Cell Signalling and Technology, USA. TNF-α, IL-1β and IL-6 antibodies were from Komabiotech, Korea. IL-10 and IL-23 antibodies were from Thermo Fisher Scientific, USA. LysoSensor green DND-189 was from Molecular probes, USA. Phenylhydrazine hydrochloride (PHZ) was from Fisher Scientific, USA. Commercial bilirubin and LDH estimation kits were purchased from Agappe Diagnostics Limited, India.