Cell proliferation was assessed using the Cell Counting Kit-8 (CCK-8) assays (MedChem Express, Monmouth Junction, NJ, USA). Liver cancer cells were plated in 96-well plates (10,000 cells/well) and exposed to different concentrations of pimozide (5 and 10 µM) (MedChem Express) for 48 h. Kit-provided solution was added to each well and incubated at 37°C for 4 h. Absorbance at 450 nm was detected by a multi-well plate reader (Bio-Rad Laboratories, Inc., Hercules, CA, USA). In addition, Sk-Hep1 and Huh7 cells were treated with 10 µM pimozide alone or with combination plus pan-caspase inhibitor Z-VAD-FMK (10 µM) or RIP kinase inhibitor necrostain 1 (10 µM) or 2 mM NAC (MedChem Express) for 48 h before being subjected to cell viability assay. In addition, Sk-Hep1 and Huh7 cells were treated with the 5 µM pimozide alone, sorafenib (MedChem Express) alone or combination for 48 h and then were subjected to cell viability assay.
Pimozide
Manufactured by MedChemExpress
Sourced in United States
Pimozide is a laboratory equipment product manufactured by MedChemExpress. It functions as a potent and selective dopamine receptor antagonist.
Automatically generated - may contain errors
Lab products found in correlation
Cell counting kit 8 cck 8, by MedChemExpress (1 mentions)
Sorafenib, by MedChemExpress (1 mentions)
Multi well plate reader, by Bio-Rad (1 mentions)
N acetyl cysteine (nac), by MedChemExpress (1 mentions)
Hct116, by American Type Culture Collection (1 mentions)
Hct116, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle medium, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions)
Sw620, by American Type Culture Collection (1 mentions)
Sw620, by Thermo Fisher Scientific (1 mentions)
Hct 8 cells, by National Collection of Authenticated Cell Cultures (1 mentions)
Ncm460 cell line, by INCELL (1 mentions)
2 protocols using pimozide
1
Pimozide Inhibits Liver Cancer Cell Proliferation
2
Colon Cancer Cell Line Culture and STAT5 Inhibition
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The HCT116, SW620, and RKO COAD cell lines were obtained from American Type Culture Collection (Manassas, VA, USA). HCT8 cells were obtained from the China Center for Type Culture Collection (Wuhan, China). The NCM460 cell line was purchased from INCELL (San Antonio, TX, USA). The SW620 and HCT116 cell lines were cultured in Dulbecco’s Modified Eagle Medium (Gibco, Grand Island, NY, USA), RKO cells were cultured in Minimum Essential Medium (Gibco), HCT-8 cells were cultured in RPMI-1640 (Gibco), and NCM460 cells were cultured in M300F (INCELL). All cell culture media were supplemented with 10% fetal bovine serum (Gibco) and penicillin/streptomycin (Hyclone, Logan, UT, USA), and the cells were cultured in a humidified incubator at 37°C and 5% CO2.
The STAT5 inhibitor pimozide was obtained from MedChemExpress (Monmouth Junction, NJ, USA) and used to treat the colon cancer cells at a dose of 10 μM for 24 h.
The STAT5 inhibitor pimozide was obtained from MedChemExpress (Monmouth Junction, NJ, USA) and used to treat the colon cancer cells at a dose of 10 μM for 24 h.
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