Mouse trf1

Manufactured by Thermo Fisher Scientific

Mouse TRF1 is a laboratory product used for the detection and analysis of the TRF1 protein in mouse samples. TRF1 is a key component of the telomere complex and plays a crucial role in telomere length regulation. This product provides researchers with a tool to study the expression and function of TRF1 in mouse models.

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Lab products found in correlation

Alexa fluor 488 anti rabbit igg, by Thermo Fisher Scientific (3 mentions) Dapi fluoromount g, by Southern Biotech (3 mentions) Anti mouse igg alexa fluor 555, by Thermo Fisher Scientific (3 mentions) Rabbit anti 53bp1, by Cell Signaling Technology (1 mentions) Model tcs sp8, by Leica (1 mentions) Rabbit anti 53bp1, by Thermo Fisher Scientific (1 mentions) Anti ku70, by Cell Signaling Technology (1 mentions) Anti rad51, by Cell Signaling Technology (1 mentions)

3 protocols using mouse trf1

Immunofluorescence Staining of Naïve CD4 T Cells

Cited 1 time

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Naïve CD4 T cells were isolated and cultured as described above, followed by immunofluorescence staining using a method described previously^{6 (link)}. Rabbit anti-53BP1 (Cell Signaling) and mouse TRF1 (Thermo Fisher) were used as primary antibodies and anti-rabbit IgG-Alexa Fluor 488 and anti-mouse IgG- Alexa Fluor 555 (Invitrogen) were used as secondary antibodies. Then, cells were washed and mounted with DAPI Fluoromount-G (SouthernBiotech, Birmingham, AL). Images were acquired with a confocal laser-scanning inverted microscope (Leica Confocal, Model TCS sp8, Germany).

Nguyen L.N., Zhao J., Cao D., Dang X., Wang L., Lian J., Zhang Y., Jia Z., Wu X.Y., Morrison Z., Xie Q., Ji Y., Zhang Z., El Gazzar M., Ning S., Moorman J.P, & Yao Z.Q. (2018). Inhibition of TRF2 accelerates telomere attrition and DNA damage in naïve CD4 T cells during HCV infection. Cell Death & Disease, 9(9), 900.

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Quantifying Telomere Dynamics in T Cells

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CD4 T cells were isolated and cultured as described above, followed by immunofluorescence staining using a method described previously⁶. The primary antibodies included Rabbit anti-53BP1, and mouse TRF1 (Thermo Fisher). The secondary antibodies included anti-rabbit IgG-Alexa Fluor 488 and anti-mouse IgG-Alexa Fluor 555 (Invitrogen). We also used Tel-C (TAACCC)-FITC probe for telomere staining. The cells were washed and mounted with DAPI Fluoromount-G (SouthernBiotech, Birmingham, AL). Images were acquired with a confocal laser-scanning inverted microscope (Leica Confocal, Model TCS sp8, Germany).

Dang X., Ogbu S.C., Zhao J., Nguyen L.N., Cao D., Nguyen L.N., Khanal S., Schank M., Thakuri B.K., Wu X.Y., Morrison Z.D., Zhang J., Li Z., El Gazzar M., Ning S., Wang L., Wang Z., Moorman J.P, & Yao Z.Q. (2020). Inhibition of topoisomerase IIA (Top2α) induces telomeric DNA damage and T cell dysfunction during chronic viral infection. Cell Death & Disease, 11(3), 196.

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Visualizing DNA Repair Foci in CD4 T Cells

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CD4 T cells were isolated and treated as described above, followed by immunofluorescence staining using a method described previously [26 (link)]. Rabbit anti-53BP1, anti-Ku70, anti-RAD51 or anti-Top1cc (Cell Signaling) and mouse TRF1 (Thermo Fisher) were used as primary antibodies and anti-rabbit IgG-Alexa Fluor 488 and anti-mouse IgG-Alexa Fluor 555 (Invitrogen) were used as secondary antibodies. The cells were washed and mounted with DAPI Fluoromount-G (SouthernBiotech, Birmingham, AL). Images were acquired with a confocal laser-scanning inverted microscope (Leica Confocal, Model TCS sp8, Germany).

Ji Y., Dang X., Nguyen L.N., Nguyen L.N., Zhao J., Cao D., Khanal S., Schank M., Wu X.Y., Morrison Z.D., Zou Y., El Gazzar M., Ning S., Wang L., Moorman J.P, & Yao Z.Q. (2019). Topological DNA damage, telomere attrition and T cell senescence during chronic viral infections. Immunity & Ageing : I & A, 16, 12.

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