Log In Sign up
The largest database of trusted experimental protocols

Anti cd45 apc clone hi30

Manufactured by Cytek Biosciences
Visit Supplier

Anti CD45-APC clone HI30 is a fluorescently-labeled monoclonal antibody that binds to the CD45 antigen present on the surface of human leukocytes. It can be used for the identification and enumeration of leukocyte populations in flow cytometric analysis.

Automatically generated - may contain errors

Lab products found in correlation

Fc block, by Thermo Fisher Scientific (1 mentions) Cd3 pe clone hit3a, by BioLegend (1 mentions)

Spelling variants of this product

Anti-CD45 (3 citations) CD45 (HI30; (2 citations)

2 protocols using anti cd45 apc clone hi30

1

Phagocytosis Assay for iMGLs and MD-Mϕ

Check if the same lab product or an alternative is used in the 5 most similar protocols
iMGLs and MD-Mϕ, were incubated with mouse anti CD16/32 Fc-receptor block (2 mg/ml; BD Biosciences) for 15 minutes at 4°C. Cells were then stained with anti CD45-APC clone HI30 (Tonbo Biosciences) at 1:200 in flow cytometer buffer. Samples were then analyzed using Amnis Imagestreamer®x Mark II Imaging Flow Cytometer (Millipore). E.coli, human synaptosome, fAβ, and BDTO phagocytosis was analyzed using the IDEAS software onboard Internalization Wizard algorithm. Additive free Anti-CD11b antibody (BioLegend, #301312) was used for CD11b blockade.
Abud E.M., Ramirez R.N., Martinez E.S., Healy L.M., Nguyen C.H., Newman S.A., Yeromin A.V., Scarfone V.M., Marsh S.E., Fimbres C., Caraway C.A., Fote G.M., Madany A.M., Agrawal A., Kayed R., Gylys K.H., Cahalan M.D., Cummings B.J., Antel J.P., Mortazavi A., Carson M.J., Poon W.W, & Blurton-Jones M. (2017). iPSC-derived human microglia-like cells to study neurological diseases. Neuron, 94(2), 278-293.e9.
+ Open protocol
+ Expand
2

Multiparameter Flow Cytometric Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Following selection on the autoMACS Pro, cells were incubated with 0.5% bovine serum albumin, Fc block (eBioscience/Thermo Fisher Scientific, Waltham, MA), anti-CD45-APC (clone HI30, Tonbo Biosciences, San Diego, CA), and the appropriate antibody, then washed and analyzed on a Guava 6HT flow cytometer (Millipore, Billerica, MA). Antibodies were CD3-PE (clone HIT3a, Biolegend, San Diego, CA), CD14-PE (clone 61D3, Tonbo Biosciences) and CD34-PE (clone 4H11, eBiosciences). Data was analyzed with FlowJo Software version 10 for Windows (Ashland, Oregon).
Pomicter A.D., Clair P.M., Yan D., Heaton W.L., Eiring A.M., Anderson M.B., Richards S.M., Gililland J., O’Hare T, & Deininger M.W. (2021). Femoral heads from total hip arthroplasty as a source of adult hematopoietic cells. Acta haematologica, 144(4), 458-464.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!