We employed the xCELLigence RTCA SP system (ACEA Bioscience Inc.) to assess the cytolytic capacity of different CAR-T cell variants50 (link) (Mock T, BCMA CAR-T, FCRL5 CAR-T, and FCRL5 CAR-T/IL15). FCRL5-HeLa tumor cells were seeded in an E-plate 96 (ACEA Bioscience) at a density of 104 cells per well and cultured for approximately 24 h before the introduction of CAR-T cells at an E:T ratio of 4:1. Data collection and analysis were performed in compliance with the manufacturer’s protocol using RTCA Software 2.1.
Xcelligence rtca sp system
Manufactured by Agilent Technologies
Sourced in United States
The XCELLigence RTCA SP system is a real-time cell analysis platform designed for monitoring and analyzing cell behavior in live-cell assays. The system uses electronic sensors to continuously measure changes in cell status, including cell proliferation, cytotoxicity, and cell-substrate interactions, without the need for labels or dyes.
Automatically generated - may contain errors
6 protocols using xcelligence rtca sp system
1
Cytolytic Assessment of CAR-T Cells
2
Transwell Cell Migration Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HUVECs or MDA-MB-231 cells were incubated for 8 h in serum-free media containing αEGFR-E-P125A or equimolar control proteins in the top chamber of a transwell plate (CIM-16; ACEA Biosciences, Inc., San Diego, CA, USA). The bottom chamber contained 10% FBS/EGM-2 as a chemoattractant. Impedance from a plate sensor was measured using the xCELLigence RTCA-SP system (ACEA Biosciences, Santa Clara, CA, USA) every 15 min.
3
Evaluating Cellular Response to Panobinostat
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The xCELLigence RTCA SP System (ACEA Biosciences Inc.) was used to assess the real-time analysis of the cellular response of A375, HeLa, HepG2, and Huh7 cell lines (25 (link)–27 (link)) to panobinostat treatment. 50ul of complete media was used to blank the 96X E-Plates, and thereafter, 104 cell/50ul (per well) were seeded into the 96X E-Plates and monitored with impedance-based xCELLigence System until a log growth forming a monolayer is obtained (approximately 18-24 hours). Increasing concentration of panobinostat (25, 50, and 100nM) or DMSO control was then added into each well to make 200ul complete media. The cell index (CI) was measured continuously following ≥70 hours of incubation. CI was normalized at the end of the experiment, and it represents several cellular indicators such as cell-cell attachment, cell number, or cell size, and increased CI indicates cell growth.
4
Transfection and H2O2 Treatment of DJ-1 KO SN4741 Cells
5
Real-Time Cell Growth Monitoring with xCELLigence
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The essence of the real-time growth profile is based on measuring the change in the adhesive properties of the cells as they are attached to the microelectrode-coated surface of special e-plates of xCELLigence system. Impedance measurement gives time quantitative information about the current state of cells such as cell number, viability, morphology and movement. Measurement is possible without using any label or chemicals. By eliminating the need for label or dye, it is possible to maximize the acquired data physiologically. The device’s software allows real-time monitoring of the experiment and real-time data visualization and analysis functions [37 ,38 ].
RL95-2 cells were resuspended in the culture medium and then seeded, to e-plate of xCELLigence RTCA SP system (ACEA Biosciences Inc., San Diego, CA, USA). In this experiment, different number of cells varying from 1.250 to 40.000 were used to obtain growth profile of RL95-2 cell line. xCELLigence system calculates the impedances parameter called “Cell Index (CI)”. CI values of RL95-2 were used to examine cell profile depending on proliferation and viability during 96 h. All experiments were performed at least three times.
RL95-2 cells were resuspended in the culture medium and then seeded, to e-plate of xCELLigence RTCA SP system (ACEA Biosciences Inc., San Diego, CA, USA). In this experiment, different number of cells varying from 1.250 to 40.000 were used to obtain growth profile of RL95-2 cell line. xCELLigence system calculates the impedances parameter called “Cell Index (CI)”. CI values of RL95-2 were used to examine cell profile depending on proliferation and viability during 96 h. All experiments were performed at least three times.
6
Real-time Monitoring of Cell Proliferation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The real-time monitoring of proliferation was carried out with the xCELLigence RTCA SP system (ACEA Biosciences Inc.). Proliferative growth was monitored over 72 h by recording the impedance every 30 min. The data are presented as the CI (arbitrary units), which corresponds to changes in impedance. For the CI, slope and doubling time data were generated with RTCA Software 1.2.1 (ACEA Biosciences Inc.) as described by [62 (link)].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!