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Mouse il 6 and kc cxcl8 duoset elisa kits

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The Mouse IL-6 and KC/CXCL8 DuoSet ELISA kits are immunoassay reagents for the quantitative determination of mouse IL-6 and KC/CXCL8 proteins in cell culture supernatants, serum, and plasma.

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Lab products found in correlation

Collagenase, by Merck Group (2 mentions) Z1 cell counter, by Beckman Coulter (2 mentions)

Close spelling variants of this product

Mouse IL-6 DuoSet ELISA kit IL-6 DuoSet ELISA kit Mouse IL‐6 DuoSet Kit Mouse IL-6 DuoSet ELISA Mouse KC ELISA kit IL-6 DuoSet kit IL-6 DuoSet ELISA Mouse DuoSet ELISA kits Mouse IL-6 DuoSet KC ELISA kit

2 protocols using mouse il 6 and kc cxcl8 duoset elisa kits

1

Murine Lung Cell Isolation and Cytokine Quantification

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The BALF was centrifuged to pellet cells, and aliquots of the supernatant were frozen at −80°C until analysis. BALF total protein was measured using a bicinchoninic acid protein assay kit (Pierce). For lung homogenates, perfused lungs were minced, digested with collagenase (150 U/ml; Sigma-Aldrich), and filtered through a 100-μm pore size cell strainer (BD). Red blood cells were removed by hypotonic lysis. The remaining cells were pelleted, resuspended in 1 ml of sterile RPMI 1640, and quantified using an automated cell counter (Z1 cell counter, Beckmann-Coulter). Murine plasma was collected from whole blood mixed with 0.5 M EDTA, and aliquots were frozen at −80°C until analysis. Cytokines were quantified in all samples by ELISA (Mouse IL-6 and KC/CXCL8 DuoSet ELISA kits, R&D Systems).
LaFayette S.L., Houle D., Beaudoin T., Wojewodka G., Radzioch D., Hoffman L.R., Burns J.L., Dandekar A.A., Smalley N.E., Chandler J.R., Zlosnik J.E., Speert D.P., Bernier J., Matouk E., Brochiero E., Rousseau S, & Nguyen D. (2015). Cystic fibrosis–adapted Pseudomonas aeruginosa quorum sensing lasR mutants cause hyperinflammatory responses. Science Advances, 1(6), e1500199.
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2

Quantification of Murine Lung Cytokines

Check if the same lab product or an alternative is used in the 5 most similar protocols
The BALF was centrifuged to pellet cells, and aliquots of the supernatant were frozen at −80°C until analysis. BALF total protein was measured using a bicinchoninic acid protein assay kit (Pierce). For lung homogenates, perfused lungs were minced, digested with collagenase (150 U/ml; Sigma-Aldrich), and filtered through a 100-µm pore size cell strainer (BD). Red blood cells were removed by hypotonic lysis. The remaining cells were pelleted, resuspended in 1 ml of sterile RPMI 1640, and quantified using an automated cell counter (Z1 cell counter, Beckmann-Coulter). Murine plasma was collected from whole blood mixed with 0.5 M EDTA, and aliquots were frozen at −80°C until analysis. Cytokines were quantified in all samples by ELISA (Mouse IL-6 and KC/CXCL8 DuoSet ELISA kits, R&D Systems).
LaFayette S.L., Houle D., Beaudoin T., Wojewodka G., Radzioch D., Hoffman L.R., Burns J.L., Dandekar A.A., Smalley N.E., Chandler J.R., Zlosnik J.E., Speert D.P., Bernier J., Matouk E., Brochiero E., Rousseau S, & Nguyen D. (2015). Cystic fibrosis–adapted Pseudomonas aeruginosa quorum sensing lasR mutants cause hyperinflammatory responses. Science Advances, 1(6), e1500199.
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