Following the treatment of different concentrations of rubone or PTX and rubone combination therapy, total mRNA was isolated using RNeasy isolation kits (Qiagen, Valencia, CA) and 170 ng total RNA was converted to cDNA using miR-34a primer to determine miR-34a concentration. To determine protein concentration, cell protein was extracted using RIPA buffer after treatment with rubone at the doses of 5 and 10 μM for 48 h. The amount of protein was adjusted to the same concentration, transferred to PVDF membrane, incubated with primary and secondary antibodies, followed by Licor Odyssey system analysis (LI-COR Biotechnology, Lincoln, NE). The primary antibodies used in Western blot and immunohistochemistry studies were the following: anti-E-cadherin (Abcam, ab15148), anti-SIRT1 (Santa Cruz, sc-15404), anti-Cyclin D1 (Abcam, ab16663), anti-p53 (Santa Cruz, sc-6243), anti-Bax (Santa Cruz, sc-6236), anti-βactin (Santa Cruz, sc-1616), anti-TAp73 (Santa Cruz, sc-7957), anti-Elk-1 (Santa Cruz, sc-355).
Anti tap73
Manufactured by Santa Cruz Biotechnology
Anti-TAp73 is a laboratory reagent used in research applications. It functions as an antibody that specifically binds to and detects the TAp73 protein. The core function of Anti-TAp73 is to facilitate the identification and study of the TAp73 protein in various experimental settings.
Automatically generated - may contain errors
Lab products found in correlation
Bca kit, by Thermo Fisher Scientific (3 mentions)
Anti β actin, by Santa Cruz Biotechnology (1 mentions)
Anti sirt1, by Santa Cruz Biotechnology (1 mentions)
Anti elk 1, by Santa Cruz Biotechnology (1 mentions)
Rneasy isolation kit, by Qiagen (1 mentions)
Anti cyclin d1, by Abcam (1 mentions)
Anti bax, by Santa Cruz Biotechnology (1 mentions)
Anti p53, by Santa Cruz Biotechnology (1 mentions)
Anti e cadherin, by Abcam (1 mentions)
Anti p75ntr, by Abcam (1 mentions)
Anti γ tubulin, by Merck Group (1 mentions)
Anti drebrin, by Abcam (1 mentions)
Gapdh, by Beyotime (1 mentions)
Anti psd95, by Abcam (1 mentions)
2 protocols using anti tap73
1
Molecular Profiling of Rubone and PTX Treatment
2
Protein Expression Analysis by Western Blot
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Tissue homogenates were lysed in RIPA buffer (150 mM NaCl, 1% NP-40, 0.5% DOC, 0.1% SDS, 50 mM Tris, pH, 8.0) and subjected to 10% SDS–PAGE. Protein concentrations were determined using the BCA Kit (Thermo). Antibodies used were anti-p75NTR (1:5000; Abcam); anti-TAp73 (1:200, Santa Cruz Biotechnology); anti-PSD95 (1:2000; Abcam); anti-Drebrin (1:1000; Abcam); anti-JNK and anti-phospho-JNK (1:1000; Ruiying Biological); anti-Akt and anti-phospho-Akt (1:1000; Ruiying Biological); anti-γ-tubulin (1:10000; Sigma-Aldrich); GAPDH (1:5000; Beyotime).
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