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Desalted oligonucleotides

Manufactured by Integrated DNA Technologies
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Desalted oligonucleotides are synthetic DNA or RNA molecules that have been purified to remove excess salts and other impurities. They are a common laboratory product used for various applications in molecular biology, genetics, and biotechnology.

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Lab products found in correlation

T4 dna ligase, by New England Biolabs (3 mentions) Taq dna polymerase, by Meridian Bioscience (2 mentions) Phusion polymerase, by New England Biolabs (2 mentions) Restriction endonuclease, by New England Biolabs (2 mentions) Phusion and taq dna polymerases, by New England Biolabs (1 mentions) T4 polynucleotide kinase, by New England Biolabs (1 mentions) Restriction enzyme, by New England Biolabs (1 mentions) Sodium acrylate, by Merck Group (1 mentions) Dna ladder, by Thermo Fisher Scientific (1 mentions) Dnase 1, by New England Biolabs (1 mentions) Ammonium persulfate ap, by Merck Group (1 mentions) 19 1 acrylamide bis acrylamide, by Merck Group (1 mentions) Sybr gold nucleic acid gel stain, by Thermo Fisher Scientific (1 mentions) Matrigel, by Corning (1 mentions) Histrap hp 5 ml column, by GE Healthcare (1 mentions) Analytical grade inorganic chemicals, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Oligonucleotides (424 citations) Desalted oligonucleotide primers (2 citations) Desalted DNA oligonucleotides (2 citations) Standard desalted DNA oligonucleotides (2 citations)

6 protocols using desalted oligonucleotides

1

Molecular Biology Supply Procurement

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Biochemicals were purchased from Sigma-Aldrich (St. Louis, MO). Other organic and inorganic analytical-grade chemicals were from Fisher Scientific (Atlanta, GA) and Bio-Rad (Hercules, CA). Desalted oligonucleotides were from Integrated DNA Technologies (Coralville, IN). Phusion and Taq DNA polymerases, restriction enzymes, T4 polynucleotide kinase and T4 DNA ligase were from New England Biolabs (Ipswich, MA).
Hepowit N.L., de Vera I.M., Cao S., Fu X., Wu Y., Uthandi S., Chavarria N.E., Englert M., Su D., Söll D., Kojetin D.J, & Maupin-Furlow J.A. (2016). Mechanistic insight into protein modification and sulfur mobilization activities of noncanonical E1 and associated ubiquitin-like proteins of Archaea. The FEBS journal, 283(19), 3567-3586.
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2

Acrylamide-Based Hydrogel Synthesis

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Solvents and reagents were purchased from commercial sources and used as received, unless otherwise specified. Methanol (ACS reagent grade) was obtained from Fisher Scientific. Molecular biology grade acrylamide (catalogue number A9099), sodium acrylate (catalogue number 408220), 19:1 acrylamide/bis-acrylamide (catalogue number A2917) and ammonium persulfate (APS; catalogue number A3678) were purchased from Sigma-Aldrich. Ultrapure N,N,N′,N′-tetramethylethylenediamine (TEMED; catalogue number 15524010) and SYBR Gold Nucleic Acid Gel Stain (catalogue number S11494) were obtained from Thermo Scientific. Desalted oligonucleotides were purchased from Integrated DNA Technologies (IDT). Nitrogen gas (>99.999%) was used under inert conditions and supplied by an in-house gas generator. To ensure an inert condition, it was purified through a Model 1000 oxygen trap from Sigma-Aldrich (catalogue number Z290246). Reagents with unreacted acrylamide groups were stored at 4 °C or −20 °C, protected from unnecessary exposure to light. Actin protein (>95% pure, rabbit skeletal muscle, catalogue number AKL95) was obtained from Cytoskeleton and DNase I (catalogue number M0303) was obtained from New England Biolabs. Matrigel was obtained from Corning (catalogue numbers 354277 and 356231). DNA ladders were purchased from Thermo Fischer (catalogue number SM1211).
Peng Y.H., Hsiao S.K., Gupta K., Ruland A., Auernhammer G.K., Maitz M.F., Boye S., Lattner J., Gerri C., Honigmann A., Werner C, & Krieg E. (2023). Dynamic matrices with DNA-encoded viscoelasticity for cell and organoid culture. Nature Nanotechnology, 18(12), 1463-1473.
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3

Molecular Biology Reagent Sourcing

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Biochemicals were from Sigma-Aldrich (St. Louis, MO). Other organic and inorganic analytical grade chemicals were from Fisher Scientific (Atlanta, GA). Restriction endonucleases, T4 DNA ligase and Phusion polymerase were from New England Biolabs (Ipswich, MA). Taq DNA polymerase was from Bioline (Taunton, MA). Pfu DNA polymerase was from Stratagene (La Jolla, CA). Desalted oligonucleotides were from Integrated DNA Technologies (Coralville, IA). Agarose for routine analysis of DNA was from Bio-Rad Laboratories (Hercules, CA).
Hwang S., Cordova B., Chavarria N., Elbanna D., McHugh S., Rojas J., Pfeiffer F, & Maupin-Furlow J.A. (2014). Conserved active site cysteine residue of archaeal THI4 homolog is essential for thiamine biosynthesis in Haloferax volcanii. BMC Microbiology, 14, 260.
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4

Molecular Biology Reagents and Suppliers

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All biochemical and analytical-grade inorganic chemicals were purchased from Fisher Scientific (Atlanta, GA, USA), Bio-Rad (Hercules, CA, USA), and Sigma-Aldrich (St. Louis, MO, USA). DNA polymerases and modifying enzymes were from New England Biolabs (Ipswich, MA, USA). Hi-Lo DNA standards were from Minnesota Molecular, Inc (Minneapolis, MN,USA). Taq DNA polymerase was from Bioline (Taunton, MA, USA). Desalted oligonucleotides were from Integrated DNA Technologies (Coralville, IA, USA).
Dantuluri S., Wu Y., Hepowit N.L., Chen H., Chen S, & Maupin-Furlow J.A. (2016). Proteome targets of ubiquitin-like samp1ylation are associated with sulfur metabolism and oxidative stress in Haloferax volcanii. Proteomics, 16(7), 1100-1110.
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5

Biochemicals and Reagents for Molecular Biology

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Biochemicals were from Sigma-Aldrich (St. Louis, MO, USA) or Alfa Aesar by Thermo Fisher Scientific (Tewksbury, MA, USA). Other organic and inorganic analytical grade chemicals were from Fisher Scientific (Atlanta, GA, USA). Restriction endonucleases, T4 DNA ligase, and Phusion polymerase were from New England Biolabs (Ipswich, MA, USA). Taq and Pfu DNA polymerases were from Bioline (Taunton, MA, USA) and Stratagene (La Jolla, CA, USA), respectively. Desalted oligonucleotides were from Integrated DNA Technologies (Coralville, IA, USA) and Eurofins Genomics (Louisville, KY, USA). DNA sequencing was by Eton Bioscience Inc. (Research Triangle Park, NC, USA). Proteomics was performed at UF Interdisciplinary Center for Biotechnology Research (ICBR, Gainesville, FL, USA). Strep-Tactin Superflow Plus Resin (U.S. Cat. No. 1057978) was from Qiagen (Germantown, MD, USA). HisTrap HP 5 mL columns (GE Healthcare, Cat. No. 29-0510-215) were from Sigma-Aldrich. Cellulose acetate membranes, 0.2 µm, for protein filtration were from Thermo Scientific (Waltham, MA, USA). Phleomycin (Cat. No. ant-ph-1) was from InvivoGen (San Diego, CA, USA).
Jia H., Dantuluri S., Margulies S., Smith V., Lever R., Allers T., Koh J., Chen S, & Maupin-Furlow J.A. (2023). RecJ3/4-aRNase J form a Ubl-associated nuclease complex functioning in survival against DNA damage in Haloferax volcanii. mBio, 14(4), e00852-23.
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6

Molecular Biology Reagents Sourcing

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Biochemicals and analytical-grade inorganic chemicals were purchased from Fisher Scientific (Atlanta, GA), Bio-Rad (Hercules, CA), and Sigma-Aldrich (St. Louis, MO). Desalted oligonucleotides were from Integrated DNA Technologies (Coralville, IA). DNA polymerases and restriction enzymes were from New England Biolabs (Ipswich, MA) and Clontech Laboratories, Inc. (Mountain View, CA). Hi-Lo DNA standards were from Minnesota Molecular, Inc. (Minneapolis, MN).
Mondragon P., Hwang S., Kasirajan L., Oyetoro R., Nasthas A., Winters E., Couto-Rodriguez R.L., Schmid A, & Maupin-Furlow J.A. (2022). TrmB Family Transcription Factor as a Thiol-Based Regulator of Oxidative Stress Response. mBio, 13(4), e00633-22.
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