A/PR8 and A/PR8-OVAII (H1N1) (kindly provided by P. Doherty) was grown in the allantoic cavity of embryonated hen eggs and characterized at the Trudeau Institute. Mice were infected intranasally under light isoflurane anesthesia (Webster Veterinary Supply) with a sublethal (0.1 LD50) dose in 50 μl PBS. Mice received adoptively transferred T cells in 200 μL of serum-free media via intravenous or retro orbital injection and were infected on the same day.
In some experiments mice were treated with 250 μg per day of the anti-IL-2 Abs S4B6 and JES6-1A12 (Rat IgG2a) from 1–7 dpi, or with appropriate isotype control Ab (all from BioXcell). Some mice receiving anti-IL-2 Ab treatment were also given 250 μg of anti-CD122 Ab clone TM-β1 or isotype control (Rat IgG2b) from 1–7 dpi or an isotype control (Bioxcell). In other experiments mice were treated with an IL-7 receptor blocking antibody clone A7R34 (Bioxcell). All Ab was administered by intraperitoneal injection in 200 μL of PBS.
In some experiments IAV-primed mice were treated for 5 consecutive days with 2.5mg/kg of FTY720 (Cayman Chemical) dissolved in water by i.p. injection.
In some experiments mice were treated with 250 μg per day of the anti-IL-2 Abs S4B6 and JES6-1A12 (Rat IgG2a) from 1–7 dpi, or with appropriate isotype control Ab (all from BioXcell). Some mice receiving anti-IL-2 Ab treatment were also given 250 μg of anti-CD122 Ab clone TM-β1 or isotype control (Rat IgG2b) from 1–7 dpi or an isotype control (Bioxcell). In other experiments mice were treated with an IL-7 receptor blocking antibody clone A7R34 (Bioxcell). All Ab was administered by intraperitoneal injection in 200 μL of PBS.
In some experiments IAV-primed mice were treated for 5 consecutive days with 2.5mg/kg of FTY720 (Cayman Chemical) dissolved in water by i.p. injection.