Trophozoites lysates (35 µg), or purified rEhSUMO were electrophoresed in 15% sodium dodecyl sulfate-polyacrylamide gels (SDS-PAGE), transferred to nitrocellulose membranes and probed with rat α-EhSUMO (1:2000), mouse α-EhVps32 (1:1000), rabbit α-EhADH (1:1000) [34 (link)], rabbit α-EhCPADH (1:35,000) [41 (link)], mouse α-actin (1:3000) kindly donated by Dr. José Manuel Hernández (Cell Biology Department, CINVESTAV) and mouse α-EhPCNA (1:500) antibodies [70 (link)]. Membranes were washed, incubated with α-rat, α-mouse, and α-rabbit HRP-labeled secondary antibody (Sigma, 1:10,000), and revealed with ECL Prime detection reagent (GE-Healthcare, Chicago, IL, USA), according to the manufacturer´s instructions.
α rat
Manufactured by Merck Group
Sourced in United States
The α-rat is a laboratory equipment designed for precise measurement and analysis. It serves as a versatile tool for researchers and scientists. The core function of the α-rat is to provide accurate and reliable data collection, without any further interpretation or commentary on its intended use.
Automatically generated - may contain errors
Lab products found in correlation
Ecl prime detection reagent, by GE Healthcare (2 mentions)
α flag, by Merck Group (2 mentions)
α tubulin, by Abcam (2 mentions)
Ab6160, by Abcam (2 mentions)
Nupage novex 4 12 bis tris gel, by Thermo Fisher Scientific (2 mentions)
Chemidoc mp imaging system, by Bio-Rad (2 mentions)
Supersignal west pico plus chemiluminescent substrate, by Bio-Rad (2 mentions)
α mcherry, by Proteintech (2 mentions)
Rfp antibody 6g6, by Proteintech (2 mentions)
α gapdh, by Abcam (2 mentions)
4 protocols using α rat
1
Western Blot Analysis of Entamoeba Proteins
2
Western Blot Analysis of Ehvps Proteins
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Trophozoites lysates (40 µg), or purified rEhVps22, rEhVps25, and rEhVps36 were electrophoresed in 15% SDS-PAGE, transferred to nitrocellulose membranes and probed with mouse α-EhVps22 (1:1000), rat α-EhVps25 (1:2000), rabbit α-EhVps36 (1:1000), rat α-EhVps23 (1:500), rabbit α-EhVps20 (1:500), rabbit α-EhVps32 (1:500), and mouse α-actin (1:3000) antibodies. α-actin antibody was kindly donated by Dr. José Manuel Hernández (Cell Biology Department, CINVESTAV, México). Membranes were washed, incubated with α-mouse, α-rat, and α-rabbit HRP-labeled secondary antibodies (Sigma, 1: 10,000), and revealed with ECL Prime detection reagent (GE-Healthcare, Chicago, IL, USA), according to the manufacturer’s instructions.
3
Western Blot Analysis of C. elegans Proteins
4
Western Blot Analysis of C. elegans Proteins
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Protein extracts, prepared as above, were resolved on precast NuPAGE™ Novex 4-12% Bis-Tris gels (Invitrogen NP0321BOX). The proteins were transferred to a nylon membrane with the semidry transfer Thermo Scientific™ Pierce™ Power System using the Pre-Programmed Method for High MW protein. The primary antibody used were α-PRG-1 antibody6 (link) (a gift from the Mello lab), α-CSR-1 antibody61 (link), α-PGL-162 (link) and α-DEPS-138 (link) (a gift from the Strome lab), α-FLAG (F3165, Sigma), α-GAPDH (Ab125247, Abcam), α-mCherry (RFP antibody [6G6], Chromotek), α-tubulin (Ab6160, Abcam), and the secondary antibody used were α-rabbit (31460, Pierce), α-mouse (31430, Pierce), or α-Rat (A9037, Sigma) HPR antibodies. The SuperSignal™ West Pico PLUS Chemiluminescent Substrate was used to detect the signal with the ChemiDoc™ MP imaging system (Biorad).
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