Images of GFP-transfected hippocampal neurons were captured with a 20× dry lens using Leica SP8 confocal microscopy systems. Five to eight serial optical sections (Z-interval: 1 μm) were collected. The maximum projection of the serial images and subsequent analyses were performed using ImageJ (version 1.52a) software. Dendritic morphology was manually traced using the Simple Neurite Tracer56 (link) plugin embedded in ImageJ software followed by analyses of neurite number, neurite length, and dendritic tree complexity. Sholl analysis57 (link) was performed individually for each neuron from all groups. In brief, the center of the cell soma was set as the center of a series of concentric circles with radii at 10-μm intervals. Then, the intersections between the dendritic tree and the concentric circles were counted. Dendritic morphogenesis from transgenic mouse cortical neurons as indicated by Golgi staining was captured using a Leica DM6000 B compound microscope. The three-dimensional structure of dendritic trees was reconstructed by manual tracing using the filament tool embedded in Imaris (version 8.3.0). Dendrite number and length were also determined using the filament tool.
Dm6000b compound microscope
Manufactured by Leica
The Leica DM6000B is a compound microscope designed for professional laboratory applications. It features a compact and ergonomic design, with a high-quality optical system that provides clear and detailed images. The microscope is equipped with various illumination options, including LED and halogen light sources, to accommodate a wide range of sample types and magnification requirements. The DM6000B is a versatile and reliable instrument suitable for a variety of research and analysis tasks in scientific and medical settings.
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Lab products found in correlation
6 protocols using dm6000b compound microscope
1
Quantitative Dendritic Morphology Analysis
2
Soil Arthropod Specimen Preparation
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All specimens were extracted from a soil and debris sample using standard Tullgren funnels. The material was kept in 70% ethanol.
Specimens were studied using a Leica S8AP0 dissecting microscope and a Leica DM6000B compound microscope. Line-drawings were prepared using a U-DA drawing tube attached to an Olympus CX31 compound microscope. The images were generated by combining stacks of images with different focal planes using software LAS V.4.5.0.
One specimen (female paratype) was cleared in 10% KOH and mounted on to a slide (in Hoyer's medium), the other specimens being studied in cavity slides in glycerol. The terminology used follows Harvey (1992) (link). All measurements are given in millimetres. The measurements for female are given for holotype, followed by the female paratype in parentheses.
Specimens were studied using a Leica S8AP0 dissecting microscope and a Leica DM6000B compound microscope. Line-drawings were prepared using a U-DA drawing tube attached to an Olympus CX31 compound microscope. The images were generated by combining stacks of images with different focal planes using software LAS V.4.5.0.
One specimen (female paratype) was cleared in 10% KOH and mounted on to a slide (in Hoyer's medium), the other specimens being studied in cavity slides in glycerol. The terminology used follows Harvey (1992) (link). All measurements are given in millimetres. The measurements for female are given for holotype, followed by the female paratype in parentheses.
3
Dextran Injection for Hemolymph Tracing in Flies
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Live animals were injected with fluorescently labeled 3,000 MW Dextrans (Life Technologies, Grand Island, NY, Texas Red, lysine conjugated (cat. # D-3328) or neutrally charged (cat. # D-3329); results did not differ). Dextrans were dissolved in 1× PBS at 10 mg/ml, centrifuged 5 min to remove undissolved dye, aliquoted, stored at −20°C. Flies were anesthetized on CO2 and mounted on agarose. A volume of 20–50 nl Dextrans were injected into the abdominal hemolymph with an Eppendorf FemtoJet and Femtotip needles (Eppendorf NA, Hauppauge, NY) (Supplementary Figure S1 shows exact position). Fluorescence in the wing vein hemolymph was observed in real time with epifluorescence microscopy on a Leica DM6000B compound microscope.
4
Laser-Assisted Nerve Ablation in Drosophila
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Axotomy was performed with a MicroPoint laser system48 , mounted to a Leica DM6000B compound microscope. The pulsed nitrogen pumped tunable dye laser was optimized to efficiently cut nerves on adult Drosophila. The dye cell was filled with Coumarin 440 and a dichroic for GFP was used for ablations. A wavelength of 440 nm was found optimal for ablation in adult flies, and of the wing nerve. The dichroic for GFP was found best for ablations on adult Drosophila due to the autofluorescence of the cuticle. The laser was fired using a foot pedal. Ablations were done manually without the use of a camera to guide the laser due to the large number of axons and width of the target nerve. Ablations were performed in the absence of damage: excessive power causing damage was readily seen as blebbing or physical damage to the wing. The Leica DM6000B microscope was equipped with a 10× dry objective for finding the target to be ablated and a 63× water-dipping lens for ablation itself.
5
Immunohistochemistry of Zebrafish Fin Sections
6
Morphological Analysis of Chironomidae Specimens
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For the morphological analyses of adults, pupae and larvae, the examined specimens were slide-mounted in Euparal following the procedures outlined by Saether (1969) . This ensured comparable measurements and observation of morphological characters. The morphological terminology and abbreviations used follow Saether (1980) and specimens were identified under compound microscope using available taxonomic revisions and species descriptions (Cranston 2000 (Cranston , 2007;; Ekrem 2001b Ekrem , 2002;; Ekrem et al. 2003; Glover 1973; Lindeberg 1963 Lindeberg , 1967;; Reiss & Fittkau 1971; Sanseverino 2006; Sasa 1980; Sasa & Kawai 1987; Sublette & Sasa 1994; Trivinho-Strixino 2012; Trivinho-Strixino et al. 2015; Vinogradova et al. 2009) .
Taxonomic descriptions of Chironomidae most often include and refer to line drawings of characteristic body parts. However, digital photography of diagnostic characters, including male genitalia, has proven effective in some recent studies on Chironomidae (Przhiboro & Ekrem 2011; Stur & Ekrem 2011; Stur & Ekrem 2015) . Thus, I used this approach to accurately display body coloration, wing setation and genitalia structures. Photo were taken using a Leica DFC420 camera mounted on a Leica DM6000 B compound microscope.
Taxonomic descriptions of Chironomidae most often include and refer to line drawings of characteristic body parts. However, digital photography of diagnostic characters, including male genitalia, has proven effective in some recent studies on Chironomidae (Przhiboro & Ekrem 2011; Stur & Ekrem 2011; Stur & Ekrem 2015) . Thus, I used this approach to accurately display body coloration, wing setation and genitalia structures. Photo were taken using a Leica DFC420 camera mounted on a Leica DM6000 B compound microscope.
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