Anti n cadherin rabbit polyclonal

Cell extracts were prepared with RIPA buffer containing proteases and phosphatases inhibitors. For extracting cytoplasmic, nuclear, membrane and cytoskeletal proteins separately Compartimental Protein Extraction Kit (Merk Millipore, Darmstadt, Germany) was used according with manufacturer instruction. Proteins were separated on SDS-polyacrylamide gels, transferred to nitrocellulose membranes and then treated with the following primary antibodies: anti-β-catenin mouse monoclonal (1:1000), anti-N-cadherin rabbit polyclonal (1:1000) (Santa Cruz Biotechnology), anti-E-cadherin mouse monoclonal (1:500) (Dako Corp.), anti-VEGF rabbit polyclonal (1:200) (Abcam, Inc.) antibodies. Anti-GAPDH rabbit polyclonal antibody (1:2000) (Santa Cruz Biotechnology) was used to normalize protein content. Horseradish peroxide-conjugated goat anti-mouse or goat anti-rabbit secondary antibody complexes were detected by chemiluminescence (Santa Cruz Biotechnology).

Cell extracts were prepared with RIPA buffer containing proteases and phosphatases inhibitors. Proteins were separated on SDS-polyacrylamide gels, transferred to nitrocellulose membranes, and then treated with the following primary antibodies: anti-Fibronectin mouse monoclonal (1:1000) anti-N-cadherin rabbit polyclonal (1:1000; Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), anti-Mn-SOD and anti-HO-1 (1:500; Stressgen Biotechnology Corporation, CA, USA), anti-actin α-Smooth Muscle monoclonal (1:1000) and anti-Catalase (1:1000; Sigma Aldrich), anti-VEGF rabbit polyclonal (1:200; Abcam, Inc., Cambridge UK), and anti-PPARγ (1:500; Cell Signaling Technology, MA, USA). Anti-β-tubulin I mouse monoclonal antibody (1:5000; Sigma Aldrich) was used to normalize protein content. Horseradish peroxide-conjugated goat anti-mouse or goat anti-rabbit secondary antibody complexes were detected by chemiluminescence (Cell Signaling Technology). Imaging and densitometry analysis were performed with the UVITEC Mini HD9 acquisition system (Alliance UVItec Ltd., Cambridge, UK).