Lungs from M. tuberculosis-infected (14 wpi) and age-matched uninfected (control) mice were harvested and imaged in sealed transparent polycarbonate containers using an IVIS Lumina II imaging system (PerkinElmer, Waltham, MA) 24 h after intravenous injection of MMPSense® 680 Fluorescence Imaging Agent (PerkinElmer), an in vivo imaging agent activated primarily by MMP-2, MMP-3, MMP-9 and MMP-13 (Jager et al., 2016 (link)). The data were analyzed using Living Image software (PerkinElmer).
Mmpsense 680 fluorescence imaging agent
Manufactured by PerkinElmer
Sourced in United States
The MMPSense® 680 Fluorescence Imaging Agent is a fluorescent probe designed to detect the activity of matrix metalloproteinases (MMPs) in biological samples. It is a non-invasive tool that can be used for in vitro and in vivo imaging applications.
Automatically generated - may contain errors
2 protocols using mmpsense 680 fluorescence imaging agent
1
Quantifying Pulmonary MMP Activity in TB
2
In Vivo Lung Imaging with MMP Fluorescence
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Each mouse or rat was intravenously injected with 0.2 ml (4 nmol) MMP Sense680 Fluorescence Imaging Agent (Perkin-Elmer, Waltham, MA, USA) [70 (link)]. After 24 h, animals were killed and whole lung was dissected for ex vivo imaging with MS FX-Pro Imaging System (Carestream, Rochester, NY, USA). The parameters used in imaging acquisition were as following: peak emission filter, 700 nm; excitation filter, 650 nm; acquisition time, 30 s.
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