Total cell lysates were analyzed by SDS PAGE and immunoblotting. Primary antibodies used for western blotting were as follows: ATP5a, UQCR2, SDHB, COII, NDUFB8 (Abcam 110411, 1: 1000); 49KDa (home made, 1: 500); NDUFA9 (Abcam 14713, 1: 1000); H3 (Abcam 10799, 1: 10 000); Twinkle (home made, 1: 1000); TFAM (home made, 1: 5000); exoG (Proteintech 21523-1-AP, 1: 500); MGME1 (Sigma HPA040913, 1: 200); a-tubulin (Sigma T9026, 1: 5000); endoG (Abcam 9647, 1: 1000); Fen1 (home made, 1: 1000); PDH (MitoSciences MSP07, 1: 2500); Tomm20 (Abcam 56783, 1: 500); LC3B (Abcam 48394, 1: 2000); PINK1 (Abcam 75487, 1:1000). Secondary antibodies were as follows: Rabbit (HRP) anti-mouse (Sigma A9044, 1: 10 000) and Goat (HRP) anti-rabbit (Abliance BL2407, 1: 10 000). The detection of proteins was performed using Luminata forte western HRP Substrate (Millipore) or SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Scientific). The relative abundance of specific proteins was evaluated by densitometric quantification of signal intensity, and analyzed using Quantity One image analysis software (BioRad).
Endog
Manufactured by Abcam
EndoG is a laboratory protein that is involved in DNA fragmentation and cell death. It plays a role in the mitochondrial apoptosis pathway. EndoG is commonly used in research for its enzymatic activity related to DNA cleavage.
Automatically generated - may contain errors
Lab products found in correlation
Supersignal west femto maximum sensitivity substrate, by Thermo Fisher Scientific (1 mentions)
Quantity one image analysis software, by Bio-Rad (1 mentions)
Luminata forte western hrp substrate, by Merck Group (1 mentions)
Tomm20, by Abcam (1 mentions)
Ndufb8, by Abcam (1 mentions)
Ndufa9, by Abcam (1 mentions)
Mgme1, by Merck Group (1 mentions)
Confocal laser scanning microscope, by Leica (1 mentions)
2 protocols using endog
1
Quantitative Mitochondrial Protein Analysis
2
Immunofluorescence Analysis of DNA Damage
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Cells were incubated with antibodies against γH2AX, caspase-3 (#80312, #9662, Cell Signaling Technology) or EndoG (#ab9647, Abcam) overnight at 4℃, then incubated with Alexa Fluor 488 or 594 secondary antibody (Proteintech). Nuclei were counterstained using DAPI reagent (Yeasen). Images were captured using a confocal laser scanning microscope (Leica Microsystems).
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