Cells were fixed and permeabilized for flow cytometry using the FIX & PERM Cell Permeabilization Kit (Thermo Fisher). The following mouse anti-human conjugated antibodies were used: APC-Cy7-CD16, PE-Cy7-CD57, PE-CD94, APC-HLA-G, APC-HLA-E (BioLegend), BV421-CD56, BV510-NKG2A, PE-TNF, PE-Cy7-IFNγ and AlexaFluor647-NKG2C (R&D Systems). The mouse anti-hantavirus mAB A1C5 was coupled with a secondary AlexaFlour488 antibody using a commercially available labeling kit (AlexaFluor488 Antibody Labeling Kit, Thermo Fisher). Live cells were identified using 7-AAD (BD Biosciences) or a LIVE/DEAD cell viability assay (Thermo Fisher). FACS analysis was performed using the FACSCanto2 platform, FACSDiva version 6.1.2, and Flowjo version 7.2.5 (BD Biosciences).
Apc hla g
Manufactured by BioLegend
Sourced in United States
APC-HLA-G is a flow cytometry reagent that detects the human leukocyte antigen (HLA)-G protein. HLA-G is a non-classical major histocompatibility complex (MHC) class I molecule involved in immune regulation. The APC-conjugated antibody allows for the identification and quantification of HLA-G-expressing cells using flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Facsdiva version 6, by BD (1 mentions)
Facscanto 2 platform, by BD (1 mentions)
Cd16 apc cy7, by BioLegend (1 mentions)
Live dead cell viability assay, by Thermo Fisher Scientific (1 mentions)
Fix perm cell permeabilization kit, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488 antibody labeling kit, by Thermo Fisher Scientific (1 mentions)
7 aad, by BD (1 mentions)
Hla dr fitc, by BD (1 mentions)
Facscanto cytometer, by BD (1 mentions)
Attune nxt cytometer, by BD (1 mentions)
2 protocols using apc hla g
1
Multiparameter Flow Cytometry of NK Cells
2
Flow Cytometry Characterization of Transduced PMSCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were analyzed by flow cytometry as previously described32 (link),34 (link). They were stained with FITC-CD44 (560977), PE-CD44 (51-9007656), PE-CD73 (561014), APC-CD45 (560973), PE-CD31 (560983), APC-CD29 (561794), PE-CD90 (561970), PE-CD34 (550761), APC-CD105 (562408), AF647-HLA-DR (563591), and FITC-HLA-DR (555560), all from BD Biosciences, San Jose, CA, USA and APC-HLA-G (BioLegend #335909) or appropriate isotype controls (BioLegend #400221, B.D. #51-9007655, 556650, 550854, and 556655). BD™ anti-mouse Ig, κ CompBeads were used to generate compensation controls. Transduction efficiency was assessed by GFP flow cytometry analysis. Flow cytometry was performed using FACSCanto cytometer (BD Biosciences) for cell immunophenotyping and an Attune NxT cytometer (Thermo Fisher Scientific, Waltham, MA, USA) for GFP analysis. All data were further analyzed using FlowJo software (version 10) (FlowJo LLC, Ashland, OR, USA). Trilineage differentiation of PMSCs to osteogenic, adipogenic, and chondrogenic lineages before and after transduction was performed as described earlier32 (link),34 (link).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!