Biological measurements were carried out in the same laboratory using the same analyzer at the University Hospital of Nancy. Blood samples were obtained using the ACT TPOCTS analyzer (Instrumentation Laboratory, Lexington, KY, USA) for a complete blood count and the ADVIA 2120 analyzer (Siemens, Paris, France) for hemostasis. Both analyzers were calibrated daily.
Advia 2120 analyzer
Manufactured by Siemens
Sourced in Germany
The Advia 2120 analyzer is a hematology system designed for clinical laboratory use. It performs automated analysis of blood samples, providing accurate and reliable results for a variety of hematological parameters.
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Lab products found in correlation
6 protocols using advia 2120 analyzer
1
Biological Measurements at University Hospital
2
Comprehensive Blood Analysis Protocol
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Patients’ complete blood count (CBC) and blood biochemistry were analyzed on Advia2120 analyzer (SIEMENS, Germany) and AU5800 (Beckman Coulter, United States) and Clinical Chemistry Set-ups, respectively, at the EMC laboratory within 1 h after collection. High-performance liquid chromatography (HPLC) analysis of hemoglobin variant distribution was performed by Variant II analyzer (BIO-RAD, United States). The presented normal ranges are as accepted at the EMC, if not indicated differently.
3
Stroke Biomarker Analysis Protocol
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Among stroke cases, blood sampling was performed within 24–48 hr of the stroke event. Venous blood samples were drawn between 8:30 and 10:30 a.m. after an overnight fast. CRP, total cholesterol, HDL, and LDL were analyzed with standard assays on aDxC 700 AU analyzer (Beckman Coulter). Counts of white blood cell (WBC), neutrophil, and lymphocyte were analyzed on an Advia 2120 analyzer (Siemens Diagnostics). IL‐6 was determined with chemiluminescent microparticle immunoassay on a MLX Microtiter luminometer (Dynex Technologies).
4
Blood Cell Quantification Protocol
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Whole blood was obtained by retro-orbital venipuncture collected into EDTA. Blood was diluted 1:1 with PBS, and full blood counts were performed on an ADVIA 2120 analyzer (Siemens Healthineers, Erlangen, Germany). Total cell counts from harvest organs were performed on Vi-CELL XR Viability Analyzer (Beckman Coulter, Brea, Calif).
5
Comprehensive Blood Analysis in Horses
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A complete blood cell count was performed for each horse at each time point using
an Advia 2120 analyzer (Siemens). A serum biochemistry profile including
albumin, globulin, and SAA measurement was obtained using a Cobas 6000 analyzer
(Roche).
an Advia 2120 analyzer (Siemens). A serum biochemistry profile including
albumin, globulin, and SAA measurement was obtained using a Cobas 6000 analyzer
(Roche).
6
Maternal and Neonatal Data Collection
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We recorded the following data: maternal age, pre-pregnancy body mass index (BMI), gravidity, parity, and family history of diabetes and hypertension. The number of gestational weeks was calculated based on the date of the last menstrual period, and was confirmed by ultrasound results. Data on neonatal outcomes were collected at the time of delivery. All of these data were entered into an electronic database.
All samples were collected in EDTA anticoagulant. The CBC was performed with an Advia 2120 analyzer (Siemens Medical Solutions Diagnostics, Tarrytown, NY, USA). Hemoglobin electrophoresis was carried out using a Capillarys 2 Flex Piercing analyzer (SEBIA Inc., Norcross, GA, USA) within one week after the blood sample was taken. Blood glucose levels were measured using a cobas 8000 series automatic biochemical analyzer (Roche Diagnostics, Risch-Rotkreuz, Switzerland) at gestational weeks 8–10, 20–24, 30–32, and 36–38. Ferritin was measured at gestational weeks 36–38 using chemiluminescence immunoassay (Advia Centaur, Siemens Medical Solutions Diagnostics). The HbA1c level was measured with a D10 fully automated glycosylated hemoglobin analyzer (Bio-Rad, Hercules, CA, USA) at gestational weeks 36–38.
All samples were collected in EDTA anticoagulant. The CBC was performed with an Advia 2120 analyzer (Siemens Medical Solutions Diagnostics, Tarrytown, NY, USA). Hemoglobin electrophoresis was carried out using a Capillarys 2 Flex Piercing analyzer (SEBIA Inc., Norcross, GA, USA) within one week after the blood sample was taken. Blood glucose levels were measured using a cobas 8000 series automatic biochemical analyzer (Roche Diagnostics, Risch-Rotkreuz, Switzerland) at gestational weeks 8–10, 20–24, 30–32, and 36–38. Ferritin was measured at gestational weeks 36–38 using chemiluminescence immunoassay (Advia Centaur, Siemens Medical Solutions Diagnostics). The HbA1c level was measured with a D10 fully automated glycosylated hemoglobin analyzer (Bio-Rad, Hercules, CA, USA) at gestational weeks 36–38.
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