After 21 d of osteogenic induction culture, cells were fixed with 4% paraformaldehyde (PFA, Servicebio Biotechnology Co., Ltd., Wuhan, China) and stained with alizarin red S staining (Solarbio Biotechnology Co., Ltd., Beijing, China) solution for 30 min, and neutral resin was used to seal the slices for observation using Nikon fully automatic orthomosaic microscope (Nikon, Tokyo, Japan).
Fully automatic orthomosaic microscope
Manufactured by Nikon
Sourced in Japan
The Nikon fully automatic orthomosaic microscope is a scientific instrument designed for producing high-quality, seamless composite images from multiple individual microscope images. It automatically captures and combines multiple overlapping images to create a comprehensive overview of a sample or specimen. The core function of this microscope is to generate detailed, high-resolution orthomosaic images for scientific analysis and documentation purposes.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using fully automatic orthomosaic microscope
1
Alizarin Red Staining for Osteogenesis
2
Alcian Blue Staining of Chondrogenic Cells
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After 21 d of chondrogenesis induction, cells were fixed with 4% PFA, soaked in alcian acidified solution for 3 min, then stained with alcian staining solution for 30 min, rinsed, and re-stained into nuclear solid red staining solution for 5 min, and sealed with neutral resin for observation using Nikon fully automatic orthomosaic microscope (Nikon, Tokyo, Japan).
3
Lipid Accumulation Analysis
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After 21 d of lipid-forming induction culture, cells were fixed with 4% PFA and stained with 0.3% Oil Red O staining (Solarbio Biotechnology Co., Ltd., Beijing, China) solution for 30 min, and neutral resin was used to seal the slices for observation using Nikon fully automatic orthomosaic microscope (Nikon, Tokyo, Japan).
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