The major materials employed in this study were recombinant murine granulocyte-macrophage colony stimulating factor (GM-CSF) (PeproTech, USA), interleukin-4 (IL-4) (PeproTech, USA), RPMI-1640 complete culture medium (supplemented with 10% fetus bovine serum (FBS) and antibiotics) (Hyclone, USA), MTT (Sigma, USA), fluorescence-labeled rat anti-mouse monoclonal CD11c, CD80, CD86, CD40, MHC-II, and the IgG isotypes (eBioscience, USA). The major equipment used in this study were a flow cytometer with analyzing software (Beckman, USA), an inverted optical microscope TH4-200 (Olympus, Japan), an incubator HEPA CLASS100 (Thermo, USA), a low-speed bench-top centrifuge BR4 (Jouan, France), a microplate reader (Bio-Rad, USA), nylon columns for isolating splenocytes, and culture dishes.
The experimental animals were C57BL/6 (H-2b) and BALB/c (H-2d) inbred mice, male, 6–8 weeks old, and weighing 18–20 g, purchased from the Animal Center of Xinjiang Medical University. The experiment was approved by the Animal Ethics Committee of the First Affiliated Hospital of Xinjiang Medical University (Approval Number: IACUC-2015721013).
The experimental animals were C57BL/6 (H-2b) and BALB/c (H-2d) inbred mice, male, 6–8 weeks old, and weighing 18–20 g, purchased from the Animal Center of Xinjiang Medical University. The experiment was approved by the Animal Ethics Committee of the First Affiliated Hospital of Xinjiang Medical University (Approval Number: IACUC-2015721013).