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P nitrophenol α d glucopyranoside

Manufactured by Merck Group
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P-nitrophenol-α-d-glucopyranoside is a chemical compound used as a substrate for the detection and quantification of enzymatic activity. It is a colorless, crystalline solid that undergoes a color change when cleaved by specific enzymes, allowing for the measurement of enzyme levels in various biological samples.

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Lab products found in correlation

Ft ir 1760x spectrophotometer, by PerkinElmer (2 mentions) Microtof mass spectrometer, by Bruker (2 mentions) Avance dpx 300 ft nmr spectrometer, by Bruker (2 mentions) Yeast α glucosidase enzyme, by Merck Group (2 mentions) 341 polarimeter, by PerkinElmer (2 mentions) Trizol reagent, by Thermo Fisher Scientific (2 mentions) Pancreatic lipase, by Merck Group (1 mentions) Spectronic 300 array spectrophotometer, by PerkinElmer (1 mentions) 4 methylumbelliferyl oleate, by Merck Group (1 mentions) Silica gel 60, by Merck Group (1 mentions) Spectronic 3000 array spectrophotometer, by Milton Roy (1 mentions) Avance 3 hd 500 nmr spectrometer, by Bruker (1 mentions) Clariostar apparatus, by BMG Labtech (1 mentions) Kanamycin sulfate, by Merck Group (1 mentions) Soluble starch, by Merck Group (1 mentions) Maltotriose, by Merck Group (1 mentions) Isomaltose, by Merck Group (1 mentions) 2 3 5 triphenyltetrazolium chloride, by Merck Group (1 mentions) Trehalose, by Merck Group (1 mentions) Palatinose, by Merck Group (1 mentions)

Close spelling variants of this product

P-nitrophenol α-D-glucopyranoside (PNPG) P-nitrophenol

4 protocols using p nitrophenol α d glucopyranoside

1

Spectroscopic Analysis and Enzyme Assays

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Optical rotations were obtained with a Perkin-Elmer 341 polarimeter (Boston, MA, USA). UV spectra were measured on a Milton Roy Spectronic 300 Array spectrophotometer, and IR was recorded on a Perkin-Elmer FT-IR 1760x spectrophotometer (Boston, MA, USA). High-resolution electrospray ionization mass spectra (HR-ESI-MS) were recorded with a Bruker micro TOF mass spectrometer (Billerica, MA, USA). NMR spectra were obtained with a Bruker Avance DPX-300 FT-NMR spectrometer (Billerica, MA, USA). Vacuum liquid chromatography (VLC) and column chromatography (CC) were performed on silica gel 60 (Merck, 70–230 µm, Darmstadt, Germany), silica gel 60 (Merck, 230–400 nm) or Sephadex LH-20 (Pharmacia, Piscataway, NJ, USA). Yeast α-glucosidase enzyme, p-nitrophenol-α-d-glucopyranoside, pancreatic lipase, and 4-methylumbelliferyl oleate were purchased from Sigma Chemical, Inc. (St. Louis, MO, USA), and acarbose from Fluka Chemical (Buchs, Switzerland).
Chatsumpun N., Sritularak B, & Likhitwitayawuid K. (2017). New Biflavonoids with α-Glucosidase and Pancreatic Lipase Inhibitory Activities from Boesenbergia rotunda. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(11), 1862.
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2

Spectroscopic Characterization of Compounds

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Optical rotations were determined with a PerkinElmer Polarimeter 341 (Boston, MA, USA). UV spectra were recorded on a Milton Roy Spectronic 3000 Array spectrophotometer (Rochester, Monroe, NY, USA). IR spectra were obtained with a PerkinElmer FT-IR 1760X spectrophotometer (Boston, MA, USA). Mass spectra were measured using a Bruker MicroTOF mass spectrometer (ESI-MS) (Billerica, MA, USA). NMR spectra were recorded on a Bruker Avance DPX-300FT NMR spectrometer or a Bruker Avance III HD 500 NMR spectrometer (Billerica, MA, USA). Yeast α-glucosidase enzyme and p-nitrophenol-α-d-glucopyranoside were obtained from Sigma Chemical, Inc. (St. Louis, MO, USA), and acarbose was purchased from Fluka Chemical (Buchs, Switzerland). Microtiter plate readings were carried out with a CLARIOstar apparatus (BMGLABTECH, Ortenberg, Germany).
Thant M.T., Sritularak B., Chatsumpun N., Mekboonsonglarp W., Punpreuk Y, & Likhitwitayawuid K. (2021). Three Novel Biphenanthrene Derivatives and a New Phenylpropanoid Ester from Aerides multiflora and Their α-Glucosidase Inhibitory Activity. Plants, 10(2), 385.
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3

Carbohydrate Utilization Assay

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Glucose, fructose and sucrose were from Merck (Darmstadt, Germany). Isomaltose, maltose, maltotriose, melezitose monohydrate, palatinose, trehalose, soluble starch, p-nitrophenol, p-nitrophenol-α-d-glucopyranoside, kanamycin sulfate, 1,4-dithiothreitol and 2,3,5-triphenyltetrazolium chloride were from Sigma-Aldrich (Saint Lois, MO, USA). Yeast extract, peptone, tryptone and agar were from Laboratorios Conda S.A. (Madrid, Spain).
Garcia-Gonzalez M., Minguet-Lobato M., Plou F.J, & Fernandez-Lobato M. (2020). Molecular characterization and heterologous expression of two α-glucosidases from Metschnikowia spp, both producers of honey sugars. Microbial Cell Factories, 19, 140.
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4

Protein Extraction and Characterization in Rat L6 Myoblasts

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α-Glucosidase, p-nitrophenol- α-D glucopyranoside, and the primers of PTP1B, GLUT4, and β-actin were purchased from Sigma-Aldrich (St. Louis, USA). PTP1B (human recombinant) was purchased from Biomol International LP (PA, USA). TRIzol Reagent and M-PER™ Mammalian Protein Extraction Reagent were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Primary antibodies, rabbitanti-GLUT4 and goat-anti-PTP1B, were purchased from Sino Biological (China). All other chemicals and reagents used were of analytical grade and purchased from VWR (Radnor, PA, USA).
Rat L6 myoblasts purchased from ATCC (Manassas, VA) were cultured in complete growth media (DMEM supplemented with 100 U/mL penicillin, 100 μg/mL streptomycin, and 10 % FBS) at 37 °C in 5 % CO2. L6 myoblasts were differentiated by the method described previously [21 (link), 22 (link)] with slight modification. Briefly, the medium was replaced with the DMEM supplemented with 2% FBS to induce differentiation when the cells reached 80–90% confluence. After 48 hours, the differentiated myotubes were serum-starved in 0.2 % BSA for 18 hours prior to the cell-based assays, except cytotoxicity assay.
Zehra S.A., Bhattarai P., Zhang J., Liu Y., Parveen Z., Sajid M, & Zhu L. (2022). In Vitro and In Vivo Evaluation of the Antidiabetic Activity of Solidago virgaurea Extracts. Current bioactive compounds, 19(4), e150622206034.
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