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Alkaline lignin

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Alkaline lignin is a type of lab equipment produced by Merck Group. It is a naturally-occurring, complex organic polymer found in the cell walls of plants. Alkaline lignin serves as a core structural component in various plant-based materials.

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Lignin (45 citations)

2 protocols using alkaline lignin

1

Crude Enzyme Production from Bacteria

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The medium used for crude enzyme production was Berg salt liquid medium, composed of 2 g NaNO3; 0.5 g K2HPO4; 0.02 g MgSO4:7H2O; 0.02 g MnSO4:7H2O; 0.02 g FeSO4:7H2O; 0.5 g CaCl2:2H2O; and 1% alkaline lignin (Sigma Aldrich) in 1L of distilled water [25 (link)]. To obtain the crude enzyme from each bacterium, it was necessary to make suspensions, incubated at 30 °C and shaken at 120 RPM for 15 days. Then, they were centrifuged at 7000 rpm for 6 min and the supernatant was separated for enzymatic evaluation.
Rodriguez-Yupanqui M., De La Cruz-Noriega M., Quiñones C., Otiniano N.M., Quezada-Alvarez M.A., Rojas-Villacorta W., Vergara-Medina G.A., León-Vargas F.R., Solís-Muñoz H, & Rojas-Flores S. (2023). Lignin-Degrading Bacteria in Paper Mill Sludge. Microorganisms, 11(5), 1168.
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2

Lignin Content Determination in Plants

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For the determination of lignin content, the plant tissues, such as the leaves and inflorescence stems of the transgenic Arabidopsis and the leaves and stems of tea plant, were collected and stored at −80°C after drying. The lignin content was determined according to previously established protocols [50 (link)]. The commercial alkaline lignin (Sigma-Aldrich, USA) was used to construct a linear calibration curve.
Yang H., Jia X., Gao T., Gong S., Xia L., Zhang P., Qi Y., Liu S., Yu Y, & Wang W. (2024). The CsmiR397a-CsLAC17 module regulates lignin biosynthesis to balance the tenderness and gray blight resistance in young tea shoots. Horticulture Research, 11(5), uhae085.
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