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Cyanine7.5 carboxylic acid

Manufactured by Lumiprobe
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Cyanine7.5 carboxylic acid is a near-infrared fluorescent dye. It has an absorption maximum at 783 nm and an emission maximum at 808 nm. The carboxylic acid functional group allows for easy conjugation to various biomolecules.

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Lab products found in correlation

Phosphate buffered saline 1x, by Corning (3 mentions) Alexa fluor 488 phalloidin, by Thermo Fisher Scientific (3 mentions) Crystal violet dye, by Thermo Fisher Scientific (2 mentions) Hplc grade methanol, by Thermo Fisher Scientific (2 mentions) 4 6 diamidino 2 phenylindole dilactate dapi, by Thermo Fisher Scientific (2 mentions) Amicon ultra centrifugal filter, by Merck Group (2 mentions) Rpmi 1640 medium, by Corning (2 mentions) Hplc grade acetonitrile, by Thermo Fisher Scientific (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Niraparib, by MedChemExpress (1 mentions) Rpmi 1640 medium, by American Type Culture Collection (1 mentions) Ct26 cells, by American Type Culture Collection (1 mentions) Neutral buffered formalin, by Merck Group (1 mentions) Glacial acetic acid, by Thermo Fisher Scientific (1 mentions) Dextran sulfate sodium salt, by Merck Group (1 mentions) Temozolomide, by MedChemExpress (1 mentions) Talazoparib, by MedChemExpress (1 mentions) Methanol, by Thermo Fisher Scientific (1 mentions) Roswell park memorial institute (rpmi), by American Type Culture Collection (1 mentions) Geltrex, by Thermo Fisher Scientific (1 mentions)

4 protocols using cyanine7.5 carboxylic acid

1

Synthesis and Characterization of A-PBzOx-A Polymer

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The POx polymer, A-PBzOx-A, was synthesized as previously described [45 (link)]. Crystal violet dye, acetonitrile high-performance liquid chromatography (HPLC grade), TFA, and methanol (HPLC grade) were purchased from Fisher Scientific (Hampton, NH, USA). Cyanine 7.5 carboxylic acid was purchased from Lumiprobe (Hunt Valley, MD, USA), niraparib was purchased from MedChemExpress (Monmouth Junction, NJ, USA) and HS-173 was purchased from MedChemExpress and Selleck Chem (Houston, TX, USA). Anti-CTLA-4 antibody (9D9 clone) was purchased from Bio X Cell (West Lebanon, NH). Phosphate-buffered saline (PBS) 1X and RPMI 1640 medium were purchased from Corning Inc. (Corning, NY, USA). Amicon Ultra centrifugal filters and 10% (v/v) neutral buffered formalin were obtained from Millipore Sigma (Burlington, MA, USA). DAPI (4′,6-Diamidino-2-Phenylindole, Dilactate) and Alexa Fluor™ 488 Phalloidin were purchased from Thermo Fisher Scientific (Carlsbad, CA, USA). CT26 cells were purchased from ATCC and maintained in RPMI 1640 medium supplemented with 10% (v/v) fetal bovine serum and 1% (v/v) penicillin/streptomycin.
Landry M.R., DuRoss A.N., Neufeld M.J., Hahn L., Sahay G., Luxenhofer R, & Sun C. (2020). Low dose novel PARP-PI3K inhibition via nanoformulation improves colorectal cancer immunoradiotherapy. Materials Today Bio, 8, 100082.
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2

Murine Cell Culture and Reagents

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Murine CT26.wt and bEnd.3 cells were purchased from ATCC and maintained in RPMI or DMEM, respectively, at 37°C and 5% CO2. Media was supplemented with 10% (v/v) fetal bovine serum and 1% (v/v) penicillin/streptomycin. Further cell authentication and Mycoplasma testing has not been performed. Cells were used within 20 passages. Talazoparib and temozolomide were purchased from MedChemExpress (Monmouth Junction, NJ, USA). HPLC grade acetonitrile and methanol were purchased from Fisher Scientific (Hampton, NH, USA). Cyanine7.5 carboxylic acid was purchased from Lumiprobe (Hunt Valley, MD, USA). Phosphate buffered saline (PBS) 1X was purchased from Corning Inc. (Corning, NY, USA). DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate), Alexa Fluor™ 488 Phalloidin, RPMI 1640, DMEM (high glucose, pyruvate) and Geltrex (LDEV-Free Reduced Growth Factor Basement Membrane Matrix) were purchased from Thermo Fisher Scientific (Carlsbad, CA, USA). Fucoidan (Fucus vesiculosus >95%) and dextran sulfate sodium salt (MW 7,000–20,000) were purchased from Sigma Aldrich (St. Louis, MO, USA). Glacial acetic acid, terephthalic acid (1,4-benzenedicarboxylic acid -BDC), DMF, hafnium (IV) chloride, and recombinant TNF alpha were purchased from Fischer Scientific.
DuRoss A.N., Landry M.R., Thomas CR J.r., Neufeld M.J, & Sun C. (2020). Fucoidan-coated Nanoparticles Target Radiation-induced P-selectin to Enhance Chemoradiotherapy in Murine Colorectal Cancer. Cancer letters, 500, 208-219.
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3

Pluronic Polymer-Based Nanoparticle Formulation

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The triblock copolymers Pluronic® F127 (Poloxamer P407 - MW12,600) and P103 (Poloxamer P333 - MW 4650) were supplied by BASF (Ludwigshafen, Germany). Coumarin 153, crystal violet dye, acetonitrile (HPLC grade), and methanol (HPLC grade) were purchased from Fisher Scientific (Hampton, NH, USA). Cyanine 7.5 Carboxylic Acid was purchased from Lumiprobe (Hunt Valley, MD, USA), Tal and Bup were purchased from MedChemExpress (Monmouth Junction, NJ, USA). Phosphate Buffered Saline (PBS) 1X and RPMI 1640 medium were purchased from Corning Inc. (Corning, NY, USA). Amicon Ultra centrifugal filters and 10% (v/v) neutral buffered formalin solution were obtained from Millipore Sigma (Burlington, MA, USA). DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate) and Alexa Fluor™ 488 Phalloidin were purchased from Thermo Fisher Scientific (Carlsbad, CA, USA). 4T1 cells were kindly donated by the Dr. Lei Xing (Stanford University, CA, USA) and maintained in RPMI 1640 medium supplemented with 10% (v/v) fetal bovine serum (FBS) and 1% (v/v) penicillin/streptomycin.
DuRoss A.N., Neufeld M.J., Landry M.R., Rosch J.G., Eaton C.T., Sahay G., Thomas CR J.r, & Sun C. (2019). Micellar Formulation of Talazoparib and Buparlisib for Enhanced DNA Damage in Breast Cancer Chemoradiotherapy. ACS applied materials & interfaces, 11(13), 12342-12356.
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4

Synthesis and Functionalization of Silver Nanostructures

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Silver
nitrate (AgNO3), hydrogen tetrachloroaurate(III) (HAuCl4·3H2O), N,N-diisopropylethylamine
(DIPEA), succinic anhydride, sodium borohydride (NaBH4),
ammonium hydroxide (NH4OH, 30%, 15.7 M), PAA (MW: 100k,
35% wt in H2O), PBS buffer, Al(NO3)3, As2O3, As2O5, CaCl2, CdCl2, CeCl3, CrBr2, Cr(NO3)3, CuCl2, FeCl2, FeCl3, HgCl2, MgCl2, Pb(NO3)2, and ZnCl2 were purchased from Sigma-Aldrich.
Glucose, sodium hydroxide (NaOH), and dimethylformamide (DMF) were
purchased from Fisher Scientific. Cyanine7.5 carboxylic acid was purchased
from Lumiprobe. All chemicals were used without further purification,
and the glassware was cleaned using aqua regia comprised of a 3:1
mixture of HCl and HNO3 by volume. Deionized water (DI-H2O) at the resistivity of 18.2 MΩ·cm was obtained
from a Milli-Q system. ProPlate 64-well multichambers were purchased
from Grace Bio-Labs. Polysine slides were purchased from Thermo Scientific,
and the slides were carboxylated before the growth of Ag-NIFs. Briefly,
the Polysine slides were ultrasonically cleaned in the order of solvent
baths of acetone, methanol, and DI-H2O for 5 min each.
Then, five slides were immersed in a DMF solution containing succinic
anhydride (0.45 g) and DIPEA (0.6 mL) for 18 h. The slides were cleaned
sequentially with EtOH and DI-H2O, and store at 4 °C
after spin-drying.
Yen H.C., Kuo T.R., Huang M.H., Huang H.K, & Chen C.C. (2020). Design of Fluorescence-Enhanced Silver Nanoisland Chips for High-Throughput and Rapid Arsenite Assay. ACS Omega, 5(31), 19771-19777.
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