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Cd197

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CD197, also known as CCR7, is a cell surface receptor that plays a key role in lymphocyte trafficking and homing. It is expressed on a variety of immune cells, including T cells, B cells, and dendritic cells. CD197 is involved in the migration of these cells to secondary lymphoid organs and sites of inflammation.

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Lab products found in correlation

Bovine serum albumin (bsa), by BioLegend (1 mentions) Human il 6, by Thermo Fisher Scientific (1 mentions) Accutase solution, by Merck Group (1 mentions) Human m csf, by BioLegend (1 mentions) Ifn γ, by Thermo Fisher Scientific (1 mentions) Human fcr blocking reagent, by BioLegend (1 mentions) Human gm csf, by BioLegend (1 mentions) Tnf α, by Thermo Fisher Scientific (1 mentions) Interleukin 4 (il 4), by BioLegend (1 mentions) Il 1β, by Thermo Fisher Scientific (1 mentions) Ethylene diamine tetraacetic acid (edta), by Merck Group (1 mentions) Cxp software, by Beckman Coulter (1 mentions) Cytomics fc500 flow cytometer, by Beckman Coulter (1 mentions) Live dead fixable blue dead cell stain kit, by Thermo Fisher Scientific (1 mentions) Cd103, by BD (1 mentions) Lsrfortessa flow cytometer, by BD (1 mentions) Cd62l, by BD (1 mentions) Novoexpress software, by Agilent Technologies (1 mentions) Tim 3, by BioLegend (1 mentions) Novocyte flow cytometer, by Agilent Technologies (1 mentions)

Close spelling variants of this product

CD197/CCR7 (clone G043H7) PE Anti-CD197 (CCR7) PE Brilliant Violet 421 anti-human CD197 CD197 (clone G043H7; Anti-CD197-APC-Cy7 (CCR7) CD197 (CCR7; G043H7; APC-CD197(CCR7) CD197 PE-CF594

5 protocols using cd197

1

Differentiation of Primary Human Myeloid Cells

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Primary human monocytes, macrophages, and dendritic cells were cultured in RPMI1640 GlutaMAX medium supplemented with 10% FCS. All media contain 1% penicillin/streptomycin, and the cells were cultured at 37 °C in a 5% CO2 atmosphere. Sc1o was dissolved in DMSO and further diluted in media (cstock = 25 mM, maximal DMSO concentration during experiments 0.3% v/v EDTA was from Sigma Aldrich (Schnelldorf, Germany). Human FcR blocking reagent, bovine serum albumin, human CD14 MicroBeads, human GM-CSF, human M-CSF, IL-4, and all antibodies for surface staining except CD197 from BioLegend (Fell, Germany) were from Miltenyi Biotec (Bergisch Gladbach, Germany). Human IL-6, IFN-γ, IL-1β, and TNF-α were from PeproTech (Hamburg, Germany). PGE2 and Accutase solution were from Merck (Darmstadt, Germany).
Blum L., Ulshöfer T., Henke M., Krieg R., Berneburg I., Geisslinger G., Becker K., Parnham M.J, & Schiffmann S. (2020). The immunomodulatory potential of the arylmethylaminosteroid sc1o. Journal of Molecular Medicine (Berlin, Germany), 99(2), 261-272.
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2

Multicolor Flow Cytometry for Cell Characterization

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Flow cytometry was carried out using distinct fluorochrome-conjugated mAb that recognize human CD4, CD8, CD56 (Beckman Coulter), CD45RA, CD197 (BioLegend), human TRBV12-3/TRBV12-4 (Beckman Coulter), and mouse CD4 and CD8 (eBioscience). These mAbs were labeled with Alexa Fluor 488, phycoerythrin (PE), phycoerythrin-Texas Red (ECD), phycoerythrin-cyanin 5 (PC5), or phycoerythrin-cyanin 7 (PC7). The cells were examined by means of multicolor flow cytometry on the Cytomics FC500 flow cytometer (Beckman Coulter), and data were analyzed with CXP software (Beckman Coulter).
Pan R.Y., Chu M.T., Wang C.W., Lee Y.S., Lemonnier F., Michels A.W., Schutte R., Ostrov D.A., Chen C.B., Phillips E.J., Mallal S.A., Mockenhaupt M., Bellón T., Tassaneeyakul W., White K.D., Roujeau J.C., Chung W.H, & Hung S.I. (2019). Identification of drug-specific public TCR driving severe cutaneous adverse reactions. Nature Communications, 10, 3569.
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3

Multiparametric Flow Cytometry of Tumor Cells

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Single tumor cell suspensions were generated by manual disruption. Two subsets of anti-human antibodies were used. Subset 1: CD45, CD3, CD25, CD69, CD62L, CD45RA, CD14, CD16, CD19 (all BD Biosciences), CD8+ (eBioscience), CD4+ and CD197 (both BioLegend). Subset 2: CD45, CD3, CD25, CD45RA, CD103, CD127, CD279, FoxP3 (all BD Bioscience), CD8+ (eBioscience) and CD4+ (BioLegend). Dead cells were stained with LIVE/DEAD Fixable Blue Dead Cell Stain Kit (Invitrogen). Samples were processed on an LSRFortessa flow cytometer (BD Biosciences) and data was analyzed as whole percentage of live singlet cells using FlowJo software 7.
Stadler C.R., Bähr-Mahmud H., Plum L.M., Schmoldt K., Kölsch A.C., Türeci Ö, & Sahin U. (2015). Characterization of the first-in-class T-cell-engaging bispecific single-chain antibody for targeted immunotherapy of solid tumors expressing the oncofetal protein claudin 6. Oncoimmunology, 5(3), e1091555.
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4

Phenotypic Analysis of Engineered hCART Cells

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We assessed the phenotype of hCART transduced with different constructs after 4 weeks of culture (n=3). Antibodies used for staining included anti-CD45, -CD3, -CD4, -CD8, -CD45RA, -CD197, -CD25, -CD127, -CD69, -41BB, -ICOS, -OX40, -LIGHT, -PD1, -TIGIT, -TIM3, -LAG3 (BioLegend). hCART were stained with cocktails of fluorescently-conjugated monoclonal antibodies (mAbs) or isotype-matched controls, cells were acquired on NovoCyte flow cytometer and analyzed using NovoExpress software (Agilent). Memory T cells were analyzed by gating for CD45RA/CD197 on CD45+/CD3+/CD4+ or CD45+/CD3+/CD8+ T cells. Co-stimulatory receptor expression was examined by gating for 41BB/ICOS/OX40/LIGHT on CD45+/CD3+/CD4+ or CD45+/CD3+/CD8+ T cells; and co-inhibitory receptor expression was examined by gating for PD-1/TIGIT/TIM3/LAG3 on CD45+/CD3+/CD4+ or CD45+/CD3+/CD8+ T cells.
Thakur A., Scholler J., Kubicka E., Bliemeister E.T., Schalk D.L., June C.H, & Lum L.G. (2021). Bispecific Antibody Armed Metabolically Enhanced Headless CAR T Cells. Frontiers in Immunology, 12, 690437.
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5

Comprehensive Immune Cell Profiling with TotalSeq

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The following human Totalseq BioLegend antibodies were included
in the pool: CD3 (cat #: 300475), CD56 (cat #: 362557), CD19 (cat #:
302259), CD11c (cat #: 371519), CD38(cat #: 102733), CD45RA, (cat #:
304157) CD123(cat #: 306037), CD127 (cat #: 351352), CD4 (cat #:
300563), CD8a (cat #: 301067), CD14( cat #: 301855), CD16( cat #:
302061), CD25 (cat #: 302643), CD45RO (cat #: 304255), CD69 (cat #:
310947), CD197 (cat #: 353247), CD161 (cat #: 339945), CD28 (custom
made, clone: CD28.2), CD27 (cat #: 302847), HLA-DR (cat #: 307659), CD57
(custom made, clone: QA17A04), CD79b (cat #: 341415), CD11a (cat #:
350615), CD34 (cat #: 343537). For cellular hashing the following
TotalSeq hashtag antibodies were purchased from BioLegend: Hashtag 1
(cat #: 394601), Hashtag 2 ( cat #: 394603), Hashtag3 (cat #: 394605),
Hashtag 4 (cat #: 394607), Hashtag 5 (cat #: 394609), Hashtag 6 (cat #:
394611), Hashtag 7 (cat #: 394613), Hashtag 8 (cat #: 394615), Hashtag 9
(cat #: 394617), Hashtag 10 ( cat #: 394619). For the complete list of
antibody barcode sequences see Tables S1E and S1F.
Stuart T., Butler A., Hoffman P., Hafemeister C., Papalexi E., Mauck WM I.I.I., Hao Y., Stoeckius M., Smibert P, & Satija R. (2019). Comprehensive integration of single-cell data. Cell, 177(7), 1888-1902.e21.
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