The in vivo cardiac function and the heart rate were assessed by transthoracic echocardiography (EUB-7500, Hitachi, Tokyo) in conscious mice as described previously19 (link),37 (link). Blood pressure data were obtained from the average of three-time measurements by the tail cuff method (MK-2000ST, Muromachi Kikai, Tokyo)18 (link).
Mk 2000st
Manufactured by Muromachi Kikai
Sourced in Japan
The MK-2000ST is a precision laboratory equipment designed for scientific and research applications. It is capable of performing accurate measurements and analyses. The core function of the MK-2000ST is to provide reliable and consistent data collection and processing.
Automatically generated - may contain errors
Lab products found in correlation
Eub 7500, by Hitachi (2 mentions)
Mk 5000rq, by Muromachi Kikai (2 mentions)
Butorphanol, by Meiji Seika Pharma (1 mentions)
Medetomidine, by Nippon Zenyaku Kogyo (1 mentions)
Midazolam, by Astellas Pharma (1 mentions)
Vevo 2100 imaging system, by Fujifilm (1 mentions)
Visualsonics vevo 2100 imaging system, by Fujifilm (1 mentions)
Advia 2400 chemistry system, by Siemens (1 mentions)
Total cholesterol, by Fujifilm (1 mentions)
Hlc 725caii, by Tosoh (1 mentions)
20 protocols using mk 2000st
1
Cardiac function assessment in mice
2
Measuring Systolic BP in Mice
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Systolic blood pressure was measured in conscious mice (WT n = 12 vs Htra3 KO n = 12) by the tail-cuff system using MK-2000ST (Muromachi Kikai, Tokyo, Japan) according to the manufacturer’s protocol.
3
Monitoring Blood Flow During Aortic Clamping
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For the measurement of blood flow before, during and after aortic clamp, we used a nonpreheating, noninvasive blood pressure, and heart rate monitor (MK-2000ST; Muromachi Kikai Co. Ltd). Using a cuff pulse sensor attached to tail arteries, we detected and measured the blood flow pattern using photoplethysmography. We measured the blood flow in 6 rats (CN:1, QN:2, CH:2, CN:1) from the induction of anesthesia to the time of death (Figure 2 , B).
4
Citrus Fruit Juice Effects on Blood Pressure
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Male C57BL6 mice (CLEA, Tokyo, Japan) were divided into five groups as follows: 1) Control (water) (C), 2) 10% CU (CU10), 3) 40% CU (CU40), 4) 10% CI (CI10), and 5) 40% CI (CI40). The animals were housed in a room in which lighting was controlled (12 hours on and 12 hours off), and room temperature was kept at 25°C. They were given a standard diet (MF, Oriental Yeast Co., Ltd., Tokyo, Japan). After drinking juice ad libitum for 2 weeks from 8 weeks of age, cuff injury was induced by polyethylene cuff placement around the femoral artery. After 2 weeks of drinking citrus fruit juice, systolic blood pressure was measured by the tail-cuff method (MK-2000ST, Muromachi Kikai, Co. Ltd., Tokyo, Japan) as described previously [17 (link)].
5
Chronic C3G Supplementation Affects Blood Pressure
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A separate group of male Wistar rats was fed a basal diet for 5 days to promote habituation. They were divided into the same two experimental groups, but treatments were repeated daily for 14 days. The vehicle group received 4 ml/kg 3% Tween 80 solution per day (n = 9) and the C3G group received 10 mg/kg C3G per day (n = 8). The mean blood pressure (MBP) was determined at three time points: before treatment, on day 7, and at the end of treatment (day 14). MBP was measured with the tail cuff method, using a MK-2000ST (Muromachi Kikai, Tokyo, Japan, Fig. 2 A). After all measurements were completed, the rats were anesthetized with an intraperitoneal injection of a mixture of medetomidine (Nippon Zenyaku Kogyo Co., Ltd., Fukushima, Japan), midazolam (Astellas Pharma Inc., Tokyo, Japan), and butorphanol (Meiji Seika Pharma Co., Ltd., Tokyo, Japan) (2.5 ml/kg BW), as previously described.(20 (link)) The aorta was removed by dissection, frozen in liquid nitrogen, and stored at −80°C until analysis.
6
Murine Blood Pressure Measurement
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After 13 wk on the diets, diastolic and systolic blood pressures and heart rate were measured using a tail-cuff system (MK-2000ST, Muromachi Kikai Co., Ltd., Tokyo, Japan) that uses a photoelectric sensor to detect blood flow in the tail. Mice were acclimated to the procedure for 7 consecutive days prior to blood pressure and heart rate recordings on day 8. For each mouse, at least 1 set of 10 measurements with 9 or more successful readings, was obtained.
7
Blood Pressure and Metabolic Markers
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Systolic blood pressure (SBP) was measured by the arm-cuff method (MK-2000ST, Muromachi
Kikai Co., Ltd. Tokyo, Japan). After the animals were fasted for 18 h, blood was obtained,
and blood glucose levels were determined using a standard glucometer (Antosense II, Daikin
Industries, Osaka, Japan).
Plasma was separated from the blood samples by centrifugation at 3,000 × g for 15 min at
4°C. Triglyceride and low-density lipoprotein (LDL) cholesterol levels were measured by
SRL Inc. (Tokyo, Japan).
Kikai Co., Ltd. Tokyo, Japan). After the animals were fasted for 18 h, blood was obtained,
and blood glucose levels were determined using a standard glucometer (Antosense II, Daikin
Industries, Osaka, Japan).
Plasma was separated from the blood samples by centrifugation at 3,000 × g for 15 min at
4°C. Triglyceride and low-density lipoprotein (LDL) cholesterol levels were measured by
SRL Inc. (Tokyo, Japan).
8
Non-Invasive Blood Pressure Measurement
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The rats were placed in plastic restrainers, and a cuff with a pneumatic pulse sensor was attached to the tail and fixed with surgical tape. Blood pressure was recorded on a non-invasive rodent blood pressure monitor (MK-2000ST, Muromachi Kikai, Japan). Blood samples were collected from the tail vein and centrifuged at 1,000 × g for 10 min to separate the serum.
9
In Vivo Cardiac Function Assessment
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The in vivo cardiac function and the heart rate were assessed by transthoracic echocardiography (EUB-7500, Hitachi, Tokyo, Japan) in conscious mice as described previously [9 (link),14 (link)]. Blood pressure data were obtained from the average of three-time measurements by the tail cuff method (MK-2000ST, Muromachi Kikai, Tokyo, Japan) [26 (link),30 (link)].
10
Aortic Root Diameter Measurement
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Systolic blood pressure was measured using a tail-cuff method (MK-2000ST; Muromachi Kikai). Ultrasonography was performed using the Vevo 2100 Imaging System (VisualSonics) while mice were anesthetized with 2.5% isoflurane. The luminal diameters of the aortic root were measured at the age of 8, 12, and 30 weeks by B-mode imaging.
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