Haake mars 3 rotational rheometer

Rheological measurement of the photocroslinkable hydrogels was performed on a Haake Mars III Rotational Rheometer (Thermo Fisher Scientific Inc., Waltham, MA). PBS solutions of DEX-MAES containing 0.05% w/v Igracure D-2959 photoinitiator were placed between a glass plate and a quartz plate separated by two 0.75 mm spacers followed by photocrosslinking via the application of UV light (2.5 mW/cm²) for 2 min. Hydrogel discs were then punched out with a 0.8 cm biopsy punch. To measure their rhelogical properties, each gel disc was loaded between two stainless steel parallel plates (0.8 cm in diameter). A dynamic frequency sweep test with a constant maximum shear strain amplitude (0.1%) over a frequency range of 0.1 – 10.47 rad/sec was used to measure the storage (G′) modulus of each hydrogel at room tempererature (N = 3).

Rheological properties of the in situ formed hydrogels were measured using a Haake Mars III Rotational Rheometer (Thermo Fisher Scientific Inc., Waltham, MA). Hydrogels were prepared by mixing the precursor solutions and pipetting them between two-glass plates separated by two 0.75 mm spacers. They were allowed to form for 10 min, and the hydrogel discs were then punched out using a 0.8 cm biopsy punch. To perform the rheology, each gel disc was placed between two stainless steel parallel plates (0.8 cm in diameter). Storage (G’) and loss (G”) moduli of each hydrogel were measured by performing a dynamic frequency sweep test with a constant maximum shear strain amplitude (0.1%) over a frequency range of 0.1 - 10 Hz at 37°C. N = 3 per condition.