Log In Sign up
The largest database of trusted experimental protocols

Sab2500450

Manufactured by Merck Group
Visit Supplier

The SAB2500450 is a laboratory equipment product. It is designed to perform a specific function in a research or analytical setting. No further details about its core function or intended use can be provided in an unbiased and factual manner within the given constraints.

Automatically generated - may contain errors

Lab products found in correlation

Ab6046, by Abcam (4 mentions) A21432, by Thermo Fisher Scientific (3 mentions) A11055, by Thermo Fisher Scientific (2 mentions) Z0622, by Agilent Technologies (2 mentions) A31573, by Thermo Fisher Scientific (2 mentions) A21202, by Thermo Fisher Scientific (2 mentions) A31572, by Thermo Fisher Scientific (1 mentions) A21447, by Thermo Fisher Scientific (1 mentions) Ab16901, by Abcam (1 mentions) α actinin, by Cell Signaling Technology (1 mentions) Phospho s6 ribosomal protein ser240 244, by Cell Signaling Technology (1 mentions) S6 ribosomal protein, by Cell Signaling Technology (1 mentions) Alexa fluor 488 conjugate, by Thermo Fisher Scientific (1 mentions) Phospho erk1 2 thr202 tyr204, by Cell Signaling Technology (1 mentions) Erk1 2, by Cell Signaling Technology (1 mentions) A21206, by Thermo Fisher Scientific (1 mentions) Ab6673, by Abcam (1 mentions)

4 protocols using sab2500450

1

Immunofluorescence Protein Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
The primary protein antibodies to GAPDH (goat pAb; SAB2500450, Sigma), β-tubulin (rabbit pAb; ab6046, Abcam), α-Actinin (rabbit mAb, 6487S, Cell Signaling Technology), ER-α (rabbit mAb; RM9101S, Thermo Fisher Scientific), and mTOR (rabbit,2983S, Cell Signaling Technologies) were used. Secondary antibodies to goat IgG prelabelled with Alexa Fluor 647 (A21447), rabbit IgG pre-labelled with Alexa Fluor 555 or 647 (A31572 and A31573) were purchased from Invitrogen. All primary antibodies were used at a 1:10 dilution in 2% TBST/BSA from stock concentrations and incubated for 2 hours at room temperature, except for anti-GAPDH and anti-ER-α, which were incubated for 3 hours at room temperature. Secondary antibodies were diluted to a 1:20 working concentration in 2% TBST/BSA from stock and incubated for 1 hour at room temperature, protected from light.
Tan K.Y, & Herr A.E. (2020). Ferguson analysis of protein electromigration during single-cell electrophoresis in an open microfluidic device. The Analyst, 145(10), 3732-3741.
+ Open protocol
+ Expand
2

Comprehensive Cell Surface Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
The primary antibodies in the surface staining characterization experiments include EpCAM-FITC (mouse, mAb, SAB4700424, Sigma), IgG-FITC (mouse, mAB, SA1–12320, Pierce), EpCAM-AlexaFluor 488 (mouse, mAb, 53–8326-42, Ebioscience), primary protein antibodies to GAPDH (goat pAb; SAB2500450, Sigma), β-Tubulin (rabbit pAb; ab6046, Abcam). Secondary antibodies to goat IgG prelabeled with Alexa Fluor 488 and 555 (A11055 and A21432) were purchased from Invitrogen.
Sinkala E., Rosàs-Canyelles E, & Herr A.E. (2019). Single-cell mobility shift electrophoresis reports protein localization to the cell membrane. The Analyst, 144(3), 972-979.
+ Open protocol
+ Expand
3

Multicolor Immunofluorescence Staining Panel

Check if the same lab product or an alternative is used in the 5 most similar protocols
The primary antibodies include GAPDH (goat pAb; SAB2500450, Sigma), βTub (rabbit pAb; ab6046, Abcam), α-actinin (rabbit mAb; 6487, Cell Signaling), HER2-3B5 (mouse mAb; ab16901, Abcam), HER2-2G11 (mouse mAb; AHO0918, Thermofisher Scientific), ERK1/2 (rabbit mAb; 4695, Cell Signaling), phospho-ERK1/2 (Thr202/Tyr204) (rabbit mAb; 4370, Cell Signaling), mTOR (rabbit mAb; 2983, Cell Signaling), panCK (rabbit pAb; Z0622, Dako), S6-ribosomal protein (mouse mAb; 2317, Cell Signaling), phospho-S6-ribosomal protein (Ser240/244) (rabbit mAb; 5364, Cell Signaling). The secondary antibodies include donkey anti-mouse IgG (H + L) (Alexa Fluor 488 conjugate, A21202, Thermofisher Scientific), donkey anti-goat IgG (H + L) (Alexa Fluor 555 conjugate, A21432, Thermofisher Scientific), and donkey anti-rabbit IgG (H + L) (Alexa Fluor 647 conjugate, A31573, Thermofisher Scientific).
Kang C.C., Ward T.M., Bockhorn J., Schiffman C., Huang H., Pegram M.D, & Herr A.E. (2018). Electrophoretic cytopathology resolves ERBB2 forms with single-cell resolution. NPJ Precision Oncology, 2, 10.
+ Open protocol
+ Expand
4

Immunohistochemical profiling of breast cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary antibodies and fold dilutions against GAPDH (1:20, goat polyclonal antibody (pAb); SAB2500450, Sigma), β-tubulin (1:10, rabbit pAb; ab6046, Abcam), EpCAM (1:10, rabbit pAb; 3599, Cell Signaling), EGFR (1:10, mouse monoclonal antibody; 2322, Cell Signaling), ER (1:10, rabbit monoclonal antibody; RM-9101-S0, ThermoScientific), HER2 (1:10, mouse monoclonal antibody; MA513105, Pierce), ERK1/2 (1:10, rabbit monoclonal antibody; 4695, Cell Signaling), eIF4E (1:10, rabbit monoclonal antibody; 2067, Cell Signaling), mTOR (1:10, rabbit monoclonal antibody; 2983, Cell Signaling), panCK (1:10, rabbit pAb; Z0622, Dako) and CK8 (1:10, mouse monoclonal antibody; C5301, Sigma) were the immunoprobes in both breast cancer cell lines (BT-20, MCF7 and SK-BR-3) and patient-derived CTCs. For analysis of the MCF7-GFP cell line, an anti-GFP antibody (ab6673, Abcam) followed by anti-goat AlexaFluor 555-conjugated secondary antibody (A21432, Invitrogen) were used. Secondary antibodies to goat IgG pre-labelled with AlexaFluor 488 and 555 (A11055 and A21432), mouse IgG pre-labelled with AlexaFluor 488, 555 and 647 (A21202, A31570 and A31571), and rabbit IgG pre-labelled with AlexaFluor 488, 555 and 647 (A21206, A31572 and A31573) were used as prepared by the vendor (Invitrogen). All secondary antibodies were applied as a 1:20 dilution.
Sinkala E., Sollier-Christen E., Renier C., Rosàs-Canyelles E., Che J., Heirich K., Duncombe T.A., Vlassakis J., Yamauchi K.A., Huang H., Jeffrey S.S, & Herr A.E. (2017). Profiling protein expression in circulating tumour cells using microfluidic western blotting. Nature Communications, 8, 14622.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!