Laser scanning confocal microscopy was applied to take an image of PrL of mPFC in unaffected hemisphere. LSM 880 Axio Observer (Carl Zeiss AG, Jena, Germany) and a Plan Apochromat 63x/1.40 Oil DIC M27 objective were employed. Optical section is 0.6μm thickness and the image covering a 101.41 × 101.41 μm area within PrL of mPFC in unaffected hemisphere of 38 sections for each rat whereby more than 300,000μm3 (area×0.6μm optical section thickness) was examined for each rat. Images were analyzed with Carl Zeiss ZEN 2.1 (blue edition) (Carl Zeiss AG, Jena, Germany). Tissue in each batch was parallel scanned. Analysis was conducted blind to sample identity on batches that had been processed together. Identified objects < 0.04 μm2 and > 1.2μm2 were excluded from analysis. Counts from multiple sections were averaged to produce a representative value for each rat. Three-dimensional reconstructions of the target field were generated and qualitatively analyzed with Carl Zeiss ZEN 2.1 (blue edition) for overlap between pCofilin and PSD-95-immunoreactive elements.
Chen R., Lyu Z., Xie D., Huang D., Chen Y, & Wu C. (2019). Long-Term Potentiation of Prelimbic Cortex Ascribed to Heat-Sensitization Responses of Moxibustion. Evidence-based Complementary and Alternative Medicine : eCAM, 2019, 9465181.
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