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Dmrc p65 plasmid

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The DmrC-p65 plasmid is a molecular biology tool designed for gene expression studies. It contains the DmrC gene, which encodes a dimerization domain that can be used to control the activity of the p65 subunit of the NF-κB transcription factor. This plasmid allows for the regulated expression of genes linked to the p65-responsive promoter.

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Lab products found in correlation

4d nucleofector x unit, by Lonza (3 mentions) Sf cell line kit, by Lonza (3 mentions) Grna plasmid, by Addgene (3 mentions)

3 protocols using dmrc p65 plasmid

1

Gene Activation in PC-3 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
For the gene activation experiments 500,000 PC-3 cells were co-transfected with a mixture of 1,000 ng of dCas9-DmrA4x fusions plasmids, 500 ng of DmrC-p65 plasmid (Addgene, #104564), and 500 ng of gRNA plasmids (Addgene, #65777) targeting the AR and IRX4 promoter regions (Supplementary Data 1) using 20 ul strip with EN-150 program on a Lonza 4-D Nucleofector X Unit with the SF Cell Line Kit (Lonza). Cells were plated into 24 well plates and complete media containing 500 μM A/C heterodimerizer (Takara Clontech) was changed 24 h after transfection. Cells were harvested 36 h post transfection for RNA isolation and gene expression analysis.
Spisak S., Tisza V., Nuzzo P.V., Seo J.H., Pataki B., Ribli D., Sztupinszki Z., Bell C., Rohanizadegan M., Stillman D.R., Alaiwi S.A., Bartels A.H., Papp M., Shetty A., Abbasi F., Lin X., Lawrenson K., Gayther S.A., Pomerantz M., Baca S., Solymosi N., Csabai I., Szallasi Z., Gusev A, & Freedman M.L. (2023). A biallelic multiple nucleotide length polymorphism explains functional causality at 5p15.33 prostate cancer risk locus. Nature Communications, 14, 5118.
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2

Gene Activation in PC-3 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
For the gene activation experiments 500,000 PC-3 cells were co-transfected with a mixture of 1,000 ng of dCas9-DmrA4x fusions plasmids, 500 ng of DmrC-p65 plasmid (Addgene, #104564), and 500 ng of gRNA plasmids (Addgene, #65777) targeting the AR and IRX4 promoter regions (Supplementary Data 1) using 20 ul strip with EN-150 program on a Lonza 4-D Nucleofector X Unit with the SF Cell Line Kit (Lonza). Cells were plated into 24 well plates and complete media containing 500 μM A/C heterodimerizer (Takara Clontech) was changed 24 h after transfection. Cells were harvested 36 h post transfection for RNA isolation and gene expression analysis.
Spisak S., Tisza V., Nuzzo P.V., Seo J.H., Pataki B., Ribli D., Sztupinszki Z., Bell C., Rohanizadegan M., Stillman D.R., Alaiwi S.A., Bartels A.H., Papp M., Shetty A., Abbasi F., Lin X., Lawrenson K., Gayther S.A., Pomerantz M., Baca S., Solymosi N., Csabai I., Szallasi Z., Gusev A, & Freedman M.L. (2023). A biallelic multiple nucleotide length polymorphism explains functional causality at 5p15.33 prostate cancer risk locus. Nature Communications, 14, 5118.
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3

Gene Activation in PC-3 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
For the gene activation experiments 500,000 PC-3 cells were co-transfected with a mixture of 1,000 ng of dCas9-DmrA4x fusions plasmids, 500 ng of DmrC-p65 plasmid (Addgene, #104564), and 500 ng of gRNA plasmids (Addgene, #65777) targeting the AR and IRX4 promoter regions (Supplementary Data 1) using 20 ul strip with EN-150 program on a Lonza 4-D Nucleofector X Unit with the SF Cell Line Kit (Lonza). Cells were plated into 24 well plates and complete media containing 500 μM A/C heterodimerizer (Takara Clontech) was changed 24 h after transfection. Cells were harvested 36 h post transfection for RNA isolation and gene expression analysis.
Spisak S., Tisza V., Nuzzo P.V., Seo J.H., Pataki B., Ribli D., Sztupinszki Z., Bell C., Rohanizadegan M., Stillman D.R., Alaiwi S.A., Bartels A.H., Papp M., Shetty A., Abbasi F., Lin X., Lawrenson K., Gayther S.A., Pomerantz M., Baca S., Solymosi N., Csabai I., Szallasi Z., Gusev A, & Freedman M.L. (2023). A biallelic multiple nucleotide length polymorphism explains functional causality at 5p15.33 prostate cancer risk locus. Nature Communications, 14, 5118.
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