Quickgene sp kit dna whole blood

Manufactured by Fujifilm

Sourced in Japan

The QuickGene SP Kit DNA whole blood is a laboratory equipment product designed for the extraction and purification of genomic DNA from whole blood samples. It provides a streamlined and automated process for obtaining high-quality DNA for various downstream applications.

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Lab products found in correlation

Abi 3730 1 automated sequencer, by Thermo Fisher Scientific (3 mentions) Taqman fluorogenic 5 nuclease assay, by Thermo Fisher Scientific (3 mentions) Bigdye terminator cycle sequencing kit version 3, by Thermo Fisher Scientific (1 mentions) Bigdye terminator cycle sequencing kit, by Thermo Fisher Scientific (1 mentions) Bigdye terminator v3.1 cycle sequencing kit, by Thermo Fisher Scientific (1 mentions) Feature extraction software 10, by Agilent Technologies (1 mentions) Stabilization and drying solution, by Agilent Technologies (1 mentions)

4 protocols using quickgene sp kit dna whole blood

Genetic Variants Analysis of HTR2A

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We analyzed several genetic variants in HTR2A gene. PCR primers were designed to amplify HTR2A fragments. PCR products were sequenced by a BigDye Terminator Cycle Sequencing Kit (version 3.1, ABI, Foster City, CA, USA), and an ABI 3730 × 1 automated sequencer (Applied Biosystems, Foster City, CA, USA). SNPs confirmed in the HTR2A gene were genotyped. Genomic DNA was drawn from 5 mL of peripheral venous blood using an isolation kit (QuickGene SP Kit DNA whole blood, Fujifilm, Tokyo, Japan). Genotyping was performed using the TaqMan fluorogenic 5′ nuclease assay (ABI) [27 (link)].
We evaluated the association between genetic variants in HTR2A and risk of hypertension in each cohort, however, some of them did not show the association of genetic variants of HTR2A and risk of hypertension. In cohort A and B, several single nucleotide polymorphisms (SNPs) which shown significant and non-significant associations of risk of hypertension were reported (e.g. rs7330636, rs9590999, rs2183057) in this study.

Choi J.R., Jeon M, & Koh S.B. (2020). Association between serotonin 2A receptor (HTR2A) genetic variations and risk of hypertension in a community-based cohort study. BMC Medical Genetics, 21, 5.

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Sequencing and Genotyping of CDH13 Gene

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PCR primers were designed to independently amplify CDH13 fragments. Primer sequences are available on request. PCR products were purified and then sequenced using a BigDye Terminator Cycle Sequencing Kit (version 3.1, ABI, Foster City, CA, USA) and an ABI 3730×1 automated sequencer (Applied Biosystems, Foster City, CA, USA). The sequencing primers were the same as those used for PCR amplification. SNPs identified in the CDH13 gene by whole gene sequencing were genotyped. Genomic DNA was extracted from 5 mL of peripheral venous blood using a commercially available isolation kit (QuickGene SP Kit DNA whole blood, Fujifilm, Tokyo, Japan). Genotyping was performed using the TaqMan fluorogenic 5' nuclease assay (ABI).

Choi J.R., Jang Y., Kim Yoon S., Park J.K., Sorn S.R., Park M.Y, & Lee M. (2015). The Impact of CDH13 Polymorphism and Statin Administration on TG/HDL Ratio in Cardiovascular Patients. Yonsei Medical Journal, 56(6), 1604-1612.

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XDH Genotyping via PCR and TaqMan

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For this study, XDH fragments were independently amplified by polymerase chain reaction (PCR). PCR products were purified and then sequenced using a BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) and an ABI 3730 × 1 automated sequencer (Applied Biosystems). SNPs identified in the XDH gene by whole gene sequencing were genotyped. Genomic DNA was extracted from 5 mL of peripheral venous blood using a commercially available isolation kit (QuickGene SP Kit DNA whole blood, Fujifilm, Tokyo, Japan). Genotyping was performed using the TaqMan fluorogenic 5’ nuclease assay (Applied Biosystems) [25 (link)].

Lee J.H., Go T.H., Lee S.H., Kim J., Huh J.H., Kim J.Y., Kang D.R., Jeong S., Koh S.B, & Choi J.R. (2019). Association between Serum Urate and Risk of Hypertension in Menopausal Women with XDH Gene. Journal of Clinical Medicine, 8(5), 738.

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Genome-Wide Copy Number Variation Analysis

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Genomic DNA was isolated from peripheral blood with QuickGene SP kit DNA whole Blood (Fujifilm, Tokyo, Japan). The aCGH experiments were conducted according to the manufacturer's protocol (Agilent Technologies, Palo Alto, CA). Briefly, 0.5 μg genomic DNA from the test and the universal reference (pooled gDNA from normal fertile men) were digested with AluI (5U) and RsaI (5U) (Promega, R6281 and R6371) at 37°C for 2 h. Digestion fragments were verified by agarose gel electrophoresis. Patient samples were labelled with Cy5-dCTP whereas the pooled reference sample was labelled with Cy3-dCTP using Agilent DNA Labelling kit (Agilent Technologies, Palo Alto, CA, USA). Labelling efficiency was determined by NanoDrop ND-1000 UV-VIS spectrophotometer. Labelled DNA was denatured and preannealed with Cot-1 DNA and Agilent blocking reagent before hybridization at 65°C for 40 h in an Agilent hybridization oven. Arrays were then washed with Agilent Oligo CGH wash buffer 1 and 2 (5188-5226), acetonitrile (Sigma-Aldrich, 271004-1L), and Stabilization and Drying Solution (Agilent, 5185-5979), according to standard procedures. Finally, slides were scanned with an Agilent scanner, and image analysis was conducted using default aCGH settings of Feature Extraction Software 10.7.3.1 (Agilent Technologies, Palo Alto, CA, USA).

He T., Zhang X., Deng H., Zhou W., Zhao X., Zhao H., Lu J., Zheng Y., Zhang C., Zhang L, & Yin A. (2017). A novel Y chromosome microdeletion potentially associated with defective spermatogenesis identified by custom array comparative genome hybridization. Reproductive biomedicine online, 34(1).

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