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Q3 system

Manufactured by Merck Group
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Sourced in United States

The Q3 system is a laboratory equipment product offered by Merck Group. It is a multimodal characterization system designed for the analysis of the size, charge, and stability of particles and molecules in solution. The core function of the Q3 system is to provide accurate measurements of these physical properties using advanced techniques such as dynamic light scattering and electrophoretic light scattering.

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Lab products found in correlation

Fmoc lys boc wang resin, by AAPPTec (3 mentions) Phenol, by Merck Group (2 mentions) Trifluoroacetic acid, by Merck Group (2 mentions) Lysotracker, by Thermo Fisher Scientific (2 mentions) Methyl tert butyl ether (mtbe), by Merck Group (2 mentions) Dichloromethane, by Merck Group (2 mentions) Dimethylformamide, by Merck Group (2 mentions) Iodine, by Merck Group (2 mentions) Bacmam 2, by Thermo Fisher Scientific (2 mentions) Bodipy tr ceramide, by Thermo Fisher Scientific (2 mentions) N n diisopropylethylamine, by Merck Group (2 mentions) Acetic anhydride, by Merck Group (2 mentions) N n diisopropylcarbodiimide, by Merck Group (2 mentions) Wang resin fmoc tyr tbu wang resin, by AAPPTec (2 mentions) Celllight peroxisome gfp, by Thermo Fisher Scientific (2 mentions) Fluo 4 calcium probe, by Thermo Fisher Scientific (2 mentions) Chlorpromazine hydrochloride, by Merck Group (2 mentions) Hydroxybenzotriazole, by Merck Group (2 mentions) O 7 azabenzotriazol 1 yl n n n n tetramethyluronium hexafluorophosphate hatu, by Merck Group (2 mentions) Vectacell rhodamine 123, by Vector Laboratories (2 mentions)

Close spelling variants of this product

Direct-Q3 UV system (52 citations) Direct-Q3 water purification system (29 citations) Direct-Q3 purification system (23 citations) Millipore-Direct Q3 UV system (6 citations) Milli-Q system Direct Q3 (5 citations) Millipore Direct-Q3-UV purification system (4 citations) Q3 ultra-pure water system (2 citations) Milli-Q3 System (2 citations) Direct Q3 UV System equipment (2 citations) Q3 water purification system (2 citations)

5 protocols using q3 system

1

Fluorenylmethyloxy carbonyl peptide synthesis

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The fluorenylmethyloxy carbonyl(Fmoc) amino acids and the Fmoc-Lys(Boc)-Wang Resin were purchased from AAPPTec (Louisville, KY, USA). All the other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) and used without further purification. Chlorin e6 (Ce6) was purchased from Frontier-Scientific (Logan, UT, USA). Water was obtained from Millipore Q3 system (Billerica, MA, USA).
Dulbecco’s modified Eagle’s medium (DMEM) culture medium, fetal calf serum, and penicillin–streptomycin were purchased from Gibco (Life Technologies, NY, USA). Lysotracker green, mitotracker green, ER tracker green, HPF, Amplex Red assay, and Calcein AM were purchased from Thermo Fisher Scientific.
Gilson R.C., Tang R., Gautam K.S., Grabowska D, & Achilefu S. (2019). Trafficking of a Single Photosensitizing Molecule to Different Intracellular Organelles Demonstrates Effective Hydroxyl Radical-Mediated Photodynamic Therapy in the Endoplasmic Reticulum. Bioconjugate chemistry, 30(5), 1451-1458.
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2

Synthesis of Inorganic Nanoparticles

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Zinc acetate dihydrate (Zn(CH3COO)2·2H2O, ACS Reagent, ≥99.0%), gadolinium acetate hydrate (Gd(CH3COO)3·H2O, 99.9% trace metals basis), manganese acetate tetrahydrate (Mn(CH3COO)2·4H2O, ACS reagent, ≥98%), sodium hydroxide (NaOH pellets, ACS Reagent, ≥97.0%), tetramethylammonium hydroxide (TMAH) pentahydrate (98.5%), and oleic acid (≥99%) were purchased from Sigma-Aldrich (Darmstadt, Germany). Methanol (Ph Eur Grade VWR Chemicals, Radnor, PA, USA) and ethanol (Sigma-Aldrich, 99%) were used as solvents. We used double-distilled water direct from a Q3 system (Millipore) along with all the chemicals as purchased, without doing further purification.
Barui S., Gerbaldo R., Garino N., Brescia R., Laviano F, & Cauda V. (2020). Facile Chemical Synthesis of Doped ZnO Nanocrystals Exploiting Oleic Acid. Nanomaterials, 10(6), 1150.
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3

Peptide Synthesis and Cellular Assays

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All the fluorenylmethyloxycarbonyl (Fmoc) amino acids, Wang resin Fmoc-Tyr(tBu)-Wang resin and Fmoc-Lys(Boc)-Wang Resin were purchased from AAPPTec (Louisville, KY, USA). Dichloromethane (DCM), acetic acid, acetic anhydride, phenol, hydroxybenzotriazole (HOBt), N,N-diisopropylethylamine (DIEA), N-trityl-1,2-ethanediamine, phenol, thioanisol, dimethylformamide (DMF), N,N’-diisopropylcarbodiimide (DIC), trifluoroacetic acid (TFA), iodine, methyl tert-butyl ether (MTBE) and O-(7-azabenzotriazol-1-yl)-N,N,N’,N’-tetramethyluronium hexafluorophosphate (HATU) were purchased from Sigma-Aldrich (St. Louis, MO, USA). For a full list of chemicals used and their details, please refer to Supplementary Table 1. Water was obtained from a Millipore Q3 system. Lysotracker, BODIPY TR Ceramide, CellLight Peroxisome-GFP, BacMam 2.0, and Fluo-4 calcium probe were purchased from Thermo Fisher Scientific (Waltham, MA). Chlorpromazine hydrochloride, filipin complex, and amiloride hydrochloride were purchased from Sigma-Aldrich (St Louis, MO, USA). Reagents for organelle staining included VectaCell Rhodamine 123 (Vector Laboratories, Burlingame, CA, USA).
Shen D., Xu B., Liang K., Tang R., Sudlow G.P., Egbulefu C., Guo K., Som A., Gilson R., Maji D., Mondal S., Habimana-Griffin L., Akers W., Li S., Liu Y., Bloch S., Kurkure S., Nussinov Z., Seidel A., Tsen S.W, & Achilefu S. (2020). Selective imaging of solid tumours via the calcium-dependent high-affinity binding of a cyclic octapeptide to phosphorylated Annexin A2. Nature biomedical engineering, 4(3), 298-313.
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4

Peptide Synthesis and Cellular Assays

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All the fluorenylmethyloxycarbonyl (Fmoc) amino acids, Wang resin Fmoc-Tyr(tBu)-Wang resin and Fmoc-Lys(Boc)-Wang Resin were purchased from AAPPTec (Louisville, KY, USA). Dichloromethane (DCM), acetic acid, acetic anhydride, phenol, hydroxybenzotriazole (HOBt), N,N-diisopropylethylamine (DIEA), N-trityl-1,2-ethanediamine, phenol, thioanisol, dimethylformamide (DMF), N,N’-diisopropylcarbodiimide (DIC), trifluoroacetic acid (TFA), iodine, methyl tert-butyl ether (MTBE) and O-(7-azabenzotriazol-1-yl)-N,N,N’,N’-tetramethyluronium hexafluorophosphate (HATU) were purchased from Sigma-Aldrich (St. Louis, MO, USA). For a full list of chemicals used and their details, please refer to Supplementary Table 1. Water was obtained from a Millipore Q3 system. Lysotracker, BODIPY TR Ceramide, CellLight Peroxisome-GFP, BacMam 2.0, and Fluo-4 calcium probe were purchased from Thermo Fisher Scientific (Waltham, MA). Chlorpromazine hydrochloride, filipin complex, and amiloride hydrochloride were purchased from Sigma-Aldrich (St Louis, MO, USA). Reagents for organelle staining included VectaCell Rhodamine 123 (Vector Laboratories, Burlingame, CA, USA).
Shen D., Xu B., Liang K., Tang R., Sudlow G.P., Egbulefu C., Guo K., Som A., Gilson R., Maji D., Mondal S., Habimana-Griffin L., Akers W., Li S., Liu Y., Bloch S., Kurkure S., Nussinov Z., Seidel A., Tsen S.W, & Achilefu S. (2020). Selective imaging of solid tumours via the calcium-dependent high-affinity binding of a cyclic octapeptide to phosphorylated Annexin A2. Nature biomedical engineering, 4(3), 298-313.
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5

Synthesis and Characterization of Cationic Surfactants

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Phosphoryl trichloride, triethylamine (dry) , ethane-1,2diol, propane-1,2-diol, benzene (dry) , 2-ethylhexan-1-ol, 2-propanol and all chemicals used in this study were of analytical reagent grade, procured from S. D. Fine Chemicals Limited, India and used without any further purification. 1-(Dimethylamino) dodecane (≥ 95％) was obtained from Acros Organics, 1-(Dimethylamino) tetradecane (≥ 95％) , 1-(Dimethylamino) hexadecane (≥ 95％) were received from Sigma-Aldrich and used as received. Eosin-Y dye and standard KCl solutions were obtained from Thomas baker (chemicals) Pvt. Ltd, India. All of the surfactant solutions were prepared with ultrapure water produced by the Millipore Q3 system with a resistivity above 1.82×105 Ω・m and the measurements were performed at 25±1℃.
Patil S.V., Patil S.A, & Pratap A.P. (2016). Diester-containing Zwitterionic Gemini Surfactants with Different Spacer and Its Impact on Micellization Properties and Viscosity of Aqueous Micellar Solution. Journal of oleo science, 65(9).
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