Cyclohexane

Trans-cinnamic acid (purity ≥99%), p-cumaric acid (purity ≥98%), trans-ferulic acid (purity ≥99%), 1-decanol (purity 99%), p-toluenesulfonic acid, anhydrous toluene, luminol, H₂O₂, Tris-HCl buffer, lucigenin, xanthine, and xanthine oxidase were purchased from Sigma-Aldrich (St. Louis, Missouri, USA). DPPP was purchased from Cayman Chemical Co., Ann Arbor, MI, USA. Ethyl acetate, cyclohexane, ethanol, and potassium hydroxide (KOH) were purchased from Carlo Erba (Cornaredo, MI, Italy). Polysorbate 80 and phenolphthalein were purchased from A.C.E.F. (Fiorenzuola d’Arda, PC, Italy). Nigella oil from black cumin (Nigella sativa L.) seeds was produced by Tre Ponti Snr company (Polverigi, AN, Italy). Extra-virgin olive (EVO) oil from the Raggia cultivar of olive drupes (Olea europaea L.) was produced in a local olive mill. Chemical characterization of the EVO oil and Nigella oils has been reported in previous works [16 (link)].

OLC (≥95% purity) was purchased from Merck (Darmstadt, Germany), and OLE (≥90% purity) and OLA (≥95% purity) from Toronto Research Chemicals (North York, ON, Canada). MeOH, acetonitrile (ACN) and formic acid were acquired from AppliChem, Panreac Quimica SLU (Barcelona, Spain). Hexane, 4-hydroxybenzaldehyde, chloroform-d, tetramethylsilane and gentisic acid were purchased from Sigma-Aldrich (St. Louis, MO, USA). 3-(4-hydroxy-3-methoxyphenyl)propionic acid was purchased from Fisher Scientific (Waltham, MA, USA) and cycloHexane from Carlo Erba (Madrid, Spain). Ultrapure water was obtained using a Milli-Q purification system (Millipore, Bedford, MA, USA).

Acetone, Acetonitrile HPLC grade, cyclohexane, ethyl acetate, dichloromethane, toluene, methanol (all with purity ≥ 99.8%) were purchased from Carlo Erba. methanol (HPLC grade, ≥99.8%), chloroform (≥99.2%), and formic acid (≥99.3%) were purchased from VWR International.