Crl 10782

Two human cancerous cell lines were used, mammary adenocarcinoma (MCF-7, ATCC^® HTB-22TM) and MDA-MB-231 breast cancer cells. Cell lines used in this work were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and stored at 150 °C in a freezing/storage solution containing 90% fetal bovine serum (FBS)/10% DMSO. To determine the selectivity of cytotoxic effect, the epithelial breast MCF-12 (ATCC^® CRL-10782™) cell line was included. DMEM/high glucose supplemented with two mM L-glutamine, 10% FBS, and 1% penicillin/streptomycin were used to cultivate cell lines. Afterward, sub-confluent cultures (80–90%) were trypsinized (Trypsin 0.05 percent/0.53 mM EDTA) and spilled according to the ATCC seeding ratio. All the cell lines were cultured at 37 °C in 5% CO₂ in a humidified atmosphere. To acquire the appropriate dose, all of the samples were dissolved in DMSO and diluted in the inappropriate medium [1 (link)].

All cell lines utilized in this study were initially obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and were maintained in freezing/storage medium containing 90% fetal bovine serum (FBS)/10% DMSO at −150 °C. Four human cancerous cell lines were used in this study, namely the cervical adenocarcinoma (HeLa, ATCC^® CCL-2™), colorectal adenocarcinoma (HCT-116, ATCC^® CCL-247™), mammary adenocarcinoma (MCF-7, ATCC^® HTB-22™) and hepatocellular carcinoma (HepG2, ATCC^® HB-8065™). For comparison of cytotoxicity, the noncancerous skin fibroblast BJ-1 (ATCC^® CRL-2522™) and epithelial breast MCF-12 (ATCC^® CRL-10782™) cell lines were used. Human amniotic epithelial (WISH, (ATCC^® CCL-25™) was used for measuring the cytoprotective activity against oxidative stress. Cell lines were cultured in DMEM/high glucose supplemented with 2 mM L-glutamine, 10% FBS and 1% penicillin/streptomycin. Then, sub-confluent cultures (80–90%) were trypsinized (Trypsin 0.05%/0.53 mM EDTA) and spilt depending on the seeding ratio recommended by ATCC [16 (link),17 (link)].