electron microscope (FE-SEM, Carl Zeiss Sigma VP, Germany) and a high-resolution
transmission electron microscope (HR-TEM, JEM-2100 (HRP)) were utilized
to analyze the morphology of the synthesized Co(OH)2 NS.
HR-TEM was used to characterize the selected area diffraction pattern
(SAED) and the lattice fringe of the Co(OH)2 NS. An X-ray
diffractometer (XRD, Philips) with a Cu Kα radiation (λ
= 1.5406 Å) and a Fourier transform infrared (FTIR) spectrophotometer
(MIDAC, M4000) were used to characterize the structural properties
of Co(OH)2 NS. To calculate IC50 values, luminescence
was read with a Tecan infinite M1000 Pro instrument (Tecan, Männedorf,
Switzerland). Annexin V was evaluated with a BD FACSCantoII instrument
(BD Biosciences, San Jose, CA). Fluorescence images were capture with
a Nikon Ti Eclipse inverted microscope (Nikon, Minato City, Tokyo,
Japan).