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Infinite m1000 pro instrument

Manufactured by Tecan
Sourced in Switzerland, United States

The Infinite M1000 Pro is a microplate reader instrument designed for multimode detection. It is capable of performing various detection modes, including absorbance, fluorescence, and luminescence measurements. The instrument is designed to accommodate a wide range of sample types and microplate formats.

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3 protocols using infinite m1000 pro instrument

1

Characterization of Cobalt Hydroxide Nanosheets

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A field emission scanning
electron microscope (FE-SEM, Carl Zeiss Sigma VP, Germany) and a high-resolution
transmission electron microscope (HR-TEM, JEM-2100 (HRP)) were utilized
to analyze the morphology of the synthesized Co(OH)2 NS.
HR-TEM was used to characterize the selected area diffraction pattern
(SAED) and the lattice fringe of the Co(OH)2 NS. An X-ray
diffractometer (XRD, Philips) with a Cu Kα radiation (λ
= 1.5406 Å) and a Fourier transform infrared (FTIR) spectrophotometer
(MIDAC, M4000) were used to characterize the structural properties
of Co(OH)2 NS. To calculate IC50 values, luminescence
was read with a Tecan infinite M1000 Pro instrument (Tecan, Männedorf,
Switzerland). Annexin V was evaluated with a BD FACSCantoII instrument
(BD Biosciences, San Jose, CA). Fluorescence images were capture with
a Nikon Ti Eclipse inverted microscope (Nikon, Minato City, Tokyo,
Japan).
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2

Amyloid Inhibition Assay with R3 Peptide

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A
stock solution of 1.5 mM R3 (trifluoroacetate salt; Bachem, Bubendorf,
Switzerland) was prepared in TFE and left overnight at room temperature.
Incubation samples were set in nonbinding, flat-bottomed black microplates
(Greiner Bio-One) in triplicate for each concentration and contained
peptide (25 μM), inhibitor (seven concentrations ranging from
30 to 0.1 μM), and ThT (10 μM) in PBS containing 1.7%
of 1,1,1-trifluoroethanol. Fluorescence was read within 4 h at 37
°C with a Tecan Infinite M1000 Pro instrument. IC50 values were calculated by means of Prism as the mean of two independent
experiments.
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3

Comprehensive Rabbit Vaccine Response Monitoring

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Rabbit blood samples for hematology and coagulation were collected before treatment and at 24 h, 48 h, 72 h, 7 days, 14 days, 21 days, and 28 days post-vaccine administration. These were analyzed for hematology or coagulation parameters on an Advia2120 Hematology Analyzer (Siemens, Beersel, Belgium) and an ACL TOP500 Coagulation Analyzer (Instrumentation Laboratory, Werfen, Zaventem, Belgium), respectively. Clinical chemistry and CRP analysis were performed on serum (1.1 mL Z-gel Microtube, Sarstedt, Berchem, Belgium) collected before treatment and at 24 h (CRP only), 48 h (clinical chemistry only), 7 days, 14 days, 21 days, and 28 days post-vaccination. Clinical chemistry was analyzed on a Cobas6000 analyzer (Roche, Mannheim, Germany), while CRP was determined in serum using a rabbit CRP enzyme-linked immunosorbent assay (ELISA; Life Diagnostics Inc., West Chester, USA) on an Infinite M1000 PRO instrument (Tecan, Mechelen, Belgium) according to the manufacturer’s instructions.
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