Hematoxylin and eosin h e stain kit

Sprague-Dawley rats, male, were retrieved from the Experimental Animal Center of Zhejiang Province. The study protocol was authorized by the Ethics Review Board of Animal Use Application of The Lishui Municipal Central Hospital (Lishui, China) in compliance with the National Institutes of Health Guidelines For the Care and Use of Experimental Animals. Prior to the experiments, the rats were placed in a room under the controlled conditions of 20–26°C, 45–55% humidity, a 12 h light/dark cycle, and freely available water and standard rat chow.
SSE (purity>99.0%), bovine serum albumin (BSA) and monocrotaline (MCT) were obtained from Sigma Aldrich (Darmstadt, Germany). SSE was dissolved in normal saline. MCT was liquefied in 1M HCL neutralized with 1M NaOH, and thinned down with standard saline. Thereafter, PH was altered to 7.2–7.4. Anti-PCNA, anti-CyclinD1, anti-MMP2, anti-MMP9, anti-Bax, and anti-Bcl-2, antibodies were purchased from Abcam (Cambridge, United Kingdom). Anti-Cleaved Caspase-3, anti-p-GSK3β, anti-GSK3β, anti-β-catenin, anti-p-AKT, anti-AKT, anti-p-ERK, anti-ERK and anti-GAPDH were retrieved from Cell Signaling Technology (Danvers, MA, United States). The hematoxylin and eosin (H&E) stain kit was obtained from Solarbio (Beijing, China). The Colorimetric TUNEL Apoptosis assay kit was purchased from Beyotime Institute of Biotechnology (Jiangsu, China).

Sodium Houttuyfonate, Hematoxylin and Eosin (HE) stain kit and Mallory’s trichrome stain kit were purchased from Solarbio Science & Technology (Shanghai, China). The antibodies phospho-AMPKα (Thr172)(40H9)Rabbit mAb#2535, AMPKα(D63G4) Rabbit mAb#5832, NF-κB p65 (D14E12) XP^® Rabbit mAb #8242, Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033 were purchased from Cell Signaling Technology (Danvers, MA, United States). The antibodies β-Actin (4D3) monoclonal mouse antibody# BS6007M was purchased from Bioworld Technology (Nanjing, China). The antibodies IL-6 mouse Antibody#53324 was purchased from R&D System Technology (Minneapolis, MN, United States). The antibodies tumor necrosis factor (TNF)-α#Rabbit ab6671, Rabbit polyclonal to TGFβ1#ab92486, Rabbit polyclonal to Collagen I#ab34710, and Rabbit polyclonal to Collagen III#ab7778 and Rabbit monoclonal [EPR16891] to GAPDH, anti-BNP antibody ab19645 were purchased from Abcam (Shanghai, China). Horseradish peroxidase (HRP) conjugated goat anti-rabbit IgG antibodies and anti-mouse IgG antibodies were purchased from Biosharp (Hefei, China). Cell counting kit-8 was purchased from Dojindo Molecular Technologies (Rockville, MD, United States). SiAMPKα was bought from Riobio technologies (Guangzhou, China). Lipofectamine^® 2000 reagent was bought from Invitrogen (Carlsbad, CA, United States).

Reagents were purchased from Sigma-Aldrich (St. Louis, MO, United States), unless otherwise indicated. Antibodies against phospho-moesin (T558), moesin, and CD34 were purchased from Abcam (Cambridge, UK). β-actin antibody was obtained from Cell Signaling Technology (Beverly, MA, United States). Biotin-free horseradish peroxidase (HRP)-conjugated secondary antibodies were purchased from Sigma (St. Louis, MO, United States). Tyramide-CY3 and FITC were obtained from Servicebio (Wuhan, China). DAPI was obtained from BestBio (Shanghai, China). RIPA Lysis Buffer, PMSF, phosphatase inhibitor cocktail, and bovine serum albumin (BSA) were all obtained from GBCBIO (Guangzhou, China). Hematoxylin and Eosin (HE) Stain Kit, Periodic Acid Schiff (PAS) Stain Kit, and Masson’s trichrome Stain Kit were purchased from Solarbio (Beijing, China).