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Anti p47

Manufactured by Santa Cruz Biotechnology
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Anti-p47 is a laboratory product used for research purposes. It is an antibody that specifically targets the p47 protein.

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Lab products found in correlation

4 6 diamidino 2 phenylindole dihydrochloride dapi, by Thermo Fisher Scientific (2 mentions) Anti cd63, by Merck Group (2 mentions) Anti human mouse myeloperoxidase mpo, by R&D Systems (2 mentions) Anti c albicans, by Merck Group (2 mentions) Anti p40, by Merck Group (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Abcam (2 mentions) Anti neutrophil elastase, by Abcam (2 mentions) Anti histone h3 citrulline r2 r8 r17, by Abcam (2 mentions) Anti flag, by Merck Group (1 mentions) Anti α smooth muscle actin, by Thermo Fisher Scientific (1 mentions) Anti gapdh, by Santa Cruz Biotechnology (1 mentions) Anti catalase, by Santa Cruz Biotechnology (1 mentions) Anti sod1, by Santa Cruz Biotechnology (1 mentions) Anti p22, by Santa Cruz Biotechnology (1 mentions) Anti brdu, by Thermo Fisher Scientific (1 mentions) Anti nox4, by Abcam (1 mentions) Anti pthr 287 camkii, by Cell Signaling Technology (1 mentions) Anti annexin 5, by Abcam (1 mentions)

Close spelling variants of this product

Anti-p47-phox (sc-17844) (2 citations)

4 protocols using anti p47

1

Immunoprecipitation and Western Blotting Protocol

Cited 3 times
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IP and Western blotting were performed as described previously [15 (link),20 (link),21 (link),38 (link),40 (link)]. For immunoprecipitation, protein G beads were prepared and incubated with the specific antibodies, including anti-Flag (Sigma, F1804), anti-VCP-C terminal (Invitrogen, MA3-004), anti-P47(Santa Cruz, sc-365215) for 1 h, and the extracts were incubated with protein G beads at 4°C overnight. Interacting proteins were then detected by Western blotting.
After incubation with the specific primary antibodies (anti-Flag (Sigma-Aldrich, St. Louis, MO, USA. F1804), and other antibodies mentioned above, including anti-VCP-C terminal, anti-P47, anti-UFD-1, anti-NPL-4; and the corresponding secondary antibodies; the detection was performed as descripted above.
Sun X., Zhou N., Ma B., Wu W., Stoll S., Lai L., Qin G, & Qiu H. (2021). Functional Inhibition of Valosin-Containing Protein Induces Cardiac Dilation and Dysfunction in a New Dominant-Negative Transgenic Mouse Model. Cells, 10(11), 2891.
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2

Antibody Detection in Cell Study

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The following antibodies were used in this study: anti-α-smooth muscle actin, anti-BrdU (Invitrogen), anti-annexin V (Abcam), anti-p-Thr287 CaMKII (Cell Signaling Technology), anti-p47, anti-catalase, anti-SOD1, anti-p22, anti-GAPDH (Santa Cruz), anti-Nox1 (BD Biosciences), anti-SOD2 (Calbiochem) and anti-Nox4 (Epitomics). The generation of the anti-CaMKII [13 (link)] and anti-ox-Met 281/282 CaMKII [9 (link)] antibodies was described previously.
Zhu L.J., Klutho P.J., Scott J.A., Xie L., Luczak E.D., Dibbern M.E., Prasad A.M., Jaffer O.A., Venema A.N., Nguyen E.K., Guan X., Anderson M.E, & Grumbach I.M. (2014). Oxidative activation of the Ca2+/calmodulin-dependent protein kinase II (CaMKII) regulates vascular smooth muscle migration and apoptosis. Vascular pharmacology, 60(2), 75-83.
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3

Neutrophil Immunofluorescence Staining

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5×104 human peripheral neutrophils were plated on glass coverslips 1 h prior to stimulation. After stimulation, cells were fixed with 2% paraformaldehyde for 20 min at 37°C. Immunofluorescence staining was performed as described elsewhere. Anti-neutrophil elastase (NE) (Abcam), 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) (Life technologies), anti-human/mouse myeloperoxidase (MPO) (R&D Systems), anti-C. albicans (Acris), anti-p40 (Millipore, 1.22), anti-p47 (Santa Cruz), anti-CD63 (Millipore, RFAC4). For lung histology dissected lungs of infected mice were fixed over night in 2% paraformaldehyde and embedded in wax for sectioning. Sections were treated with a standard antigen retrieval protocol and immunofluorescence staining as described elsewhere. Cit-H3: anti-Histone H3 (citrulline R2 + R8 + R17) (Abcam, ab5103), MPO, DAPI. Stained cells and tissues were mounted and examined with confocal microscopy. Image analysis was performed using ImageJ.
Branzk N., Lubojemska A., Hardison S.E., Wang Q., Gutierrez M.G., Brown G.D, & Papayannopoulos V. (2014). Neutrophils sense microbial size and selectively release neutrophil extracellular traps in response to large pathogens. Nature immunology, 15(11), 1017-1025.
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4

Neutrophil Immunofluorescence Staining

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5×104 human peripheral neutrophils were plated on glass coverslips 1 h prior to stimulation. After stimulation, cells were fixed with 2% paraformaldehyde for 20 min at 37°C. Immunofluorescence staining was performed as described elsewhere. Anti-neutrophil elastase (NE) (Abcam), 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) (Life technologies), anti-human/mouse myeloperoxidase (MPO) (R&D Systems), anti-C. albicans (Acris), anti-p40 (Millipore, 1.22), anti-p47 (Santa Cruz), anti-CD63 (Millipore, RFAC4). For lung histology dissected lungs of infected mice were fixed over night in 2% paraformaldehyde and embedded in wax for sectioning. Sections were treated with a standard antigen retrieval protocol and immunofluorescence staining as described elsewhere. Cit-H3: anti-Histone H3 (citrulline R2 + R8 + R17) (Abcam, ab5103), MPO, DAPI. Stained cells and tissues were mounted and examined with confocal microscopy. Image analysis was performed using ImageJ.
Branzk N., Lubojemska A., Hardison S.E., Wang Q., Gutierrez M.G., Brown G.D, & Papayannopoulos V. (2014). Neutrophils sense microbial size and selectively release neutrophil extracellular traps in response to large pathogens. Nature immunology, 15(11), 1017-1025.
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