B 290 mini spray

For the microencapsulation process, native potato starch and tara gum at a 4:1 ratio were used as the outer layer materials. These components were prepared at varying concentrations of 5%, 10%, and 20% by weight/volume (w/v). The solution for encapsulation was prepared a day in advance. As far as erythrocytes were concerned, they were made at a constant concentration of 20% (w/v). Both solutions were mixed at a 1:1 ratio and blended thoroughly using an ultraturrax device (Daihan, model HG15D, Wonju, Republic of Korea), operating at 7000 revolutions per minute for 5 min. The actual encapsulation was carried out using a B-290 mini spray (BÜCHI Labortechnik AG, Flawil, Switzerland). This process occurred at inlet temperatures of 120 °C and 140 °C and an airflow rate of 650 L/h. Following this, the encapsulated materials were collected and carefully placed into low-density bags and stored within a desiccator at a temperature of 20 °C [10 (link)].
The experimental flow diagram is shown in Figure 1, in which the abbreviations T1O, T2O, T3O, T4O, T5O, and T6O are presented to refer to the ovine erythrocyte microencapsulation treatments, and the abbreviations T1V, T2V, T3V, T4V, T5V, and T6V for the bovine erythrocyte microencapsulation treatments.

For the microencapsulation process, native potato starch and tara gum at a 4:1 ratio were used as the outer layer materials. These components were prepared at varying concentrations of 5%, 10%, and 20% by weight/volume (w/v). The solution for encapsulation was prepared a day in advance. As far as erythrocytes were concerned, they were made at a constant concentration of 20% (w/v). Both solutions were mixed at a 1:1 ratio and blended thoroughly using an ultraturrax device (Daihan, model HG15D, Wonju, Republic of Korea), operating at 7000 revolutions per minute for 5 min. The actual encapsulation was carried out using a B-290 mini spray (BÜCHI Labortechnik AG, Flawil, Switzerland). This process occurred at inlet temperatures of 120 °C and 140 °C and an airflow rate of 650 L/h. Following this, the encapsulated materials were collected and carefully placed into low-density bags and stored within a desiccator at a temperature of 20 °C [10 (link)].
The experimental flow diagram is shown in Figure 1, in which the abbreviations T1O, T2O, T3O, T4O, T5O, and T6O are presented to refer to the ovine erythrocyte microencapsulation treatments, and the abbreviations T1V, T2V, T3V, T4V, T5V, and T6V for the bovine erythrocyte microencapsulation treatments.