Physiotel telemetry system

Animals were anesthetized with intramuscular Telazol® (Fort Dodge Animal Health, New York, NY) (5mg·kg⁻¹) and xylazine (2mg·kg⁻¹), intubated, and anesthesia maintained with intravenous ketamine (0.2mg·kg⁻¹·min⁻¹) and xylazine (0.03 mg·kg⁻¹·min⁻¹). The femoral artery was catheterized, followed by a heparin bolus (5000U). Under fluoroscopic guidance, local-irritant coils were implanted in the proximal-middle right renal artery, as previously described.^{[7 (link),14 (link):]} RAS pigs were subsequently fed a 10-week standard pig chow (n=7), whereas ARAS consumed an atherogenic diet of 2% cholesterol and 15% lard (TD-93296, Harlan-Teklad) (n=7),^{[7 (link)–9 (link)]} which is considered a surrogate of early atherosclerosis. In control pigs, selective renal angiography was followed by a 10-week normal diet (Normal, n=7). Mean arterial pressure (MAP) was subsequently measured by a PhysioTel® telemetry system (Data Sciences International, St. Paul, MN) implanted at baseline in the left femoral artery and averaged in the last 2–3 days before study completion.^{[2 (link),7 (link)–9 (link)]}