Cell culture dishes plates

Human osteosarcoma cell 143B, MG63 and U2OS were maintained in Dulbecco’s modified Eagle medium (DMEM). MNNG/HOS cells were cultured in Minimum Essential Medium (MEM). These media were supplemented with 10% foetal bovine serum (FBS), 2 mM L-glutamine, penicillin (100 U/ml), and streptomycin (100 μg/ml) in a humidified atmosphere of 5% CO2 maintained at 37 °C. SJSA-1 cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 with 10% foetal bovine serum (FBS), 2 mM L-glutamine, penicillin (100 U/ml), and streptomycin (100 μg/ml) in a humidified atmosphere of 5% CO2 maintained at 37 °C. All the cells were cultured in the cell culture dishes/plates which were obtained from NEST Biotechnology Co. Ltd. (Wuxi, China). The following antibodies were used in this study: antibodies against GAPDH (Santa Cruz Biotechnology, Dallas, TX, USA; SC-25778, 1:1000), PARP (Santa Cruz Biotechnology, SC-8007, 1:1000), GRP78 (Santa Cruz Biotechnology, SC-13968, 1:1000 for WB), KLF4 (Cell Signaling Technology, #12173S, 1:500), and LAMA4 (R&D Systems, AF7340, 1:200), LINC00629 SMARTPool (horizon), Tunicamycin (TM, Lot:T7765) and thapsigargin (TG, Lot:T9033) were purchased from Sigma Chemical Co. They were dissolved in DMSO and developed in stock solutions of 3 mmol/L and 1 mmol/L for TG. Cells were treated with 3 μmol/L TM or 1 μmol/L at the indicated times.