E cadherin antibody h 108

Manufactured by Santa Cruz Biotechnology

Sourced in Denmark, United States

The E-cadherin antibody (H-108) is a research-use antibody produced by Santa Cruz Biotechnology. It is designed to detect E-cadherin, a cell-cell adhesion protein, in various sample types. The antibody can be used in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to study the expression and localization of E-cadherin in cells and tissues.

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Lab products found in correlation

Ab53066, by Abcam (1 mentions) Bx50 bs fla, by Olympus (1 mentions) Ab150073, by Abcam (1 mentions) Ab150116, by Abcam (1 mentions) Lsm 700 confocal laser scanning microscope, by Zeiss (1 mentions) Hoechst 33342, by Dojindo Laboratories (1 mentions)

Spelling variants of this product

E-cadherin (534 citations) E-cadherin antibody (17 citations) H-108 (6 citations) E-cadherin (H-108, (3 citations)

3 protocols using e cadherin antibody h 108

Immunofluorescence Analysis of Cell Markers

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Cells were grown on four-well collagen type I-coated culture slides (BD BioCoat). The slides were incubated with fibroblast activation protein (FAP) antibody (ab53066, rabbit polyclonal IgG: diluted 1:100; Abcam), α-SMA antibody (1A4, mouse monoclonal IgG; diluted 1:100; DakoCytomation, Denmark), and E-cadherin antibody (H-108, rabbit polyclonal IgG; diluted 1:100; Santa Cruz Biotechnology, Inc., USA). The secondary antibodies were anti-rabbit IgG antibody conjugated with Alexa Fluor^® 488 (ab150073, donkey polyclonal IgG, diluted 1:1000; Abcam) and an anti-mouse IgG antibody conjugated with Alexa Fluor 594^® (ab150116, goat polyclonal IgG, diluted 1:1000; Abcam). The slides were observed using an immunofluorescence microscope (BX50/BS-FLA; Olympus, Japan).

Gunjigake K., Kinoshita J., Yamaguchi T., Saito H., Fujimori D., Horiike T., Harada S., Tajima H., Ninomiya I., Ohta T, & Fushida S. (2020). Interleukin-17A derived from mast cells contributes to fibrosis in gastric cancer with peritoneal dissemination. Gastric Cancer, 24(1), 31-44.

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Immunohistochemical Analysis of Tumor Markers

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Immunohistochemical analyses were performed on the constructed TMAs or individual slides using methods and assessment criteria described elsewhere [25 (link)]. The following primary antibodies were used for IHC: EGFR (Cell Signaling Technology, Inc., Beverly, MA, USA) diluted at 1:50, phosphorylated Akt (Ser473) at 1:50, phosphorylated p44/42 MAPK (Thr202/Tyr204; E10) monoclonal antibody (Cell Signaling Technology, Inc.) at 1:50, vimentin antibody V9 (Dako/Agilent Technologies, Carpinteria, CA, USA), and E-cadherin antibody H-108 (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA). The percentage of total tumor cells within each staining intensity category [0 (no staining), 1+ (weak), 2+ (moderate), 3+ (strong)] was reported. A hybrid (H)-score was then generated based on the fraction of staining cells in each intensity category. The H-score was calculated by completing the formula (% cells of 0 intensity × 0)+(% of 1+ intensity × 1)+(% of 2+ intensity × 2)+ (% of 3+ intensity × 3), producing a final H-score with a range of 0–300 [25 (link)]. For statistical analyses, the immunohistochemical results of EGFR, E-cadherin, pMAPK, and pAkt were analyzed in a binary fashion due to the skewed distribution of the results.

Despierre E., Vergote I., Anderson R., Coens C., Katsaros D., Hirsch F.R., Boeckx B., Varella-Garcia M., Ferrero A., Ray-Coquard I., Berns E.M., Casado A., Lambrechts D, & Jimeno A. (2015). Epidermal Growth Factor Receptor (EGFR) Pathway Biomarkers in the Randomized Phase III Trial of Erlotinib Versus Observation in Ovarian Cancer Patients with No Evidence of Disease Progression after First-Line Platinum-Based Chemotherapy. Targeted oncology, 10(4), 583-596.

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Immunofluorescence Analysis of E-cadherin and Vimentin

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The assay was conducted as described previously [41 (link)]. The differences were as follows: the primary antibodies used were an E-cadherin Antibody (H-108) (Santa Cruz) and a Vimentin Antibody (E-5) (Santa Cruz). The secondary antibodies used were a Goat anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Thermo Fisher Scientific), and a Goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, TRITC (Thermo Fisher Scientific). After the reaction with the secondary antibodies, a phosphate-buffered saline (PBS) solution containing 1% Hoechst33342 (Dojindo Laboratories, Kumamoto, Japan) was added to the cells and incubated for 15 min. A Zeiss LSM 700 laser-scanning confocal microscope (Carl Zeiss, Oberkochen, Germany) was used for fluorescence microscopy.

Toma T., Tateishi H., Kawakami K., Ali T.F., Kamo M., Monde K., Nakashima Y., Fujita M, & Otsuka M. (2022). Novel Inhibitor for Downstream Targeting of Transforming Growth Factor-β Signaling to Suppress Epithelial to Mesenchymal Transition and Cell Migration. International Journal of Molecular Sciences, 23(9), 5047.

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