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6 protocols using maltose

1

Yeast Growth on Diverse Carbohydrates

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Tannin (gallotannin) was purchased from LOBA Chemie (Mumbai, India), and methyl gallate was purchased from Sigma (Steinheim, Germany). Ingredients such as peptone, yeast extract, malt extract, and carbon sources such as glucose, galactose, sucrose, maltose, lactose, raffinose, and trehalose were purchased from HiMedia (Nashik, India). The genomic DNA of yeast was extracted using a Wizard® Genomic DNA purification kit (Promega Corp., Madison, WI, United States). Nucleic acid amplifications were performed using TaKaRa Ex Taq® (Shiga, Japan). Other reagents and solvents were of analytical grade.
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2

Media for Microbial Growth Analysis

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The media components like Zobell Marine Broth, glucose, maltose, sucrose, lactose were obtained from Hi-Media, Mumbai India. Ethanol and silver nitrate were obtained from Merck India.
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3

Synthesis and Characterization of Rare-Earth Doped Nanoparticles

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All the chemicals were of analytical
grade and were used without further purification; yttrium nitrate
hexahydrate (Y(NO3)3·6H2O, 99.89%),
gadolinium nitrate hexahydrate (Gd(NO3)3·6H2O, 99.89%), and terbium nitrate hexahydrate (Tb(NO3)3·6H2O, 99.89%), PEI (with Mw = 25 000 and Mn =
10 000), TNP, 2,4-DNT, 2,6-DNT, 2,4-DNP, RDX, phenylalanine,
cysteine, isoleucine, tryptophan, glutamic acid, aspartic acid, lysine,
histidine, and glutamine were purchased from Sigma-Aldrich Inc. TNT
and RDX were purchased from HEMRL, Pune. NaCl, Na2HPO4, NaH2PO4, Na2CO3, NaHCO3, and NaOH were received from Thomas Baker. NH4F was received from Merck. Dextrose, glucose, sucrose, fructose,
lactose, and maltose were received from Himedia laboratories Pvt.
Ltd. 1,3-DNB, 4-NP, 2,4-DNB, NM, 4-NT, NB, copper oxychloride, cypermethrin,
malathion, fenvelerate, carbendazim, and chloropyrifos were purchased
from a local company and used as received. Ethanol was obtained from
Hayman Ltd. Deionized water was used throughout the experiments.
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4

Optimization of Amylase Production

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OFAT method was carried out by changing parameters of factors in a sequential manner. The factors considered for optimization were pH of the media, Incubation temperature, Carbon and nitrogen sources and presence of metal ions. The pH of the media was adjusted between 4 and 10. The incubation temperature ranged between 4 °C and 45 °C. Additional carbon sources like glucose, fructose, sucrose, dextrose, inositol, mannitol, rhamnose, maltose, lactose, trehalose, sodium citrate, arabinose (HiMedia Laboratories, Mumbai, India, LR grade) was supplemented at 10 g/L along with Starch. The effect of nitrogen source on amylase production was evaluated by replacing peptone with malt extract, beef extract, yeast extract, peptone, casitone, corn steep liquor (HiMedia Laboratories, Mumbai, India, LR grade) and inorganic nitrogen sources such as ammonium sulphate, ammonium chloride, ammonium carbonate, potassium nitrate and sodium nitrate (HiMedia Laboratories, Mumbai, India, LR grade). Various metal ions such as Fe2+, Cu2+, Mg2+, K+, Ca2+, Mn2+, Ba2+, Sn2+, Zn2+, Co2+, Ni2+at 0.5 g/L were supplemented in the media in the form of metal salts. All the experiments were performed in triplicates and the data is represented in Mean ± Standard deviation.
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5

Preparation and Characterization of Natural Deep Eutectic Solvents

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Benzyl trimethyl ammonium chloride, Bovine Serum Albumin and Naringin were procured from Sigma-Aldrich, USA. Lactose, Maltose, Fructose, Sucrose, Xylose, Sodium Sulphate, Di-Potassium Hydrogen Phosphate and Sodium Carbonate were procured from Himedia, India. The purity of each chemical used in the current investigation is provided in Table 1.

Source and purity of chemicals used in the preparation of NADES and extractive fermentation.

Chemical nameCAS Reg. no.SupplierMass % purities stated by suppliers
Benzyl trimethyl ammonium chloride (BMC)56-93-9Sigma-aldrich0.99 w/w
Bovine Serum Albumin9048-46-8Sigma-aldrich0.99 w/w
Naringin10236-47-2Sigma-aldrich0.99 w/w
Lactose (L)63-42-3Himedia0.97 w/w
Fructose (F)58-49-7Himedia0.97 w/w
Sucrose (S)57-50-1Himedia0.97 w/w
Xylose (X)609-06-3Himedia0.97 w/w
Maltose (M)69-79-4Himedia0.97 w/w
Sodium sulphate54664-61-8Himedia0.97 w/w
Di-Potassium Hydrogen Phosphate7758-11-4Himedia0.97 w/w
Sodium carbonate207-838-8Himedia0.97 w/w
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6

Characterization of Carbohydrate Interactions

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Benzyl trimethyl ammonium chloride, Bovine Serum Albumin and Naringin were procured from Sigmaaldrich, USA. Lactose, Maltose, Fructose, Sucrose, Xylose, Sodium Sulphate, Di-Potassium Hydrogen Phosphate and Sodium Carbonate were procured from Himedia, India. The purity of each chemical used in the current investigation is provided in Table 1.
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