Rabbit anti murine cox 2

Manufactured by Cayman Chemical

Rabbit anti–murine COX-2 is a primary antibody that recognizes the cyclooxygenase-2 (COX-2) protein in murine (mouse) samples. COX-2 is an enzyme involved in the production of prostaglandins and is often studied in the context of inflammation and related processes.

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Lab products found in correlation

Anti α smooth muscle actin, by Merck Group (1 mentions) Rabbit anti vegfa, by Abcam (1 mentions) Mouse anti il 6, by Santa Cruz Biotechnology (1 mentions) Rat anti mouse cd68, by Bio-Rad (1 mentions) Anti tnf α, by Santa Cruz Biotechnology (1 mentions) Anti ki67, by Abcam (1 mentions) Anti il 1β, by Abcam (1 mentions) Mouse anti β actin, by Merck Group (1 mentions) Palmitic acid, by Merck Group (1 mentions) Hrp conjugated donkey anti rabbit igg, by Promega (1 mentions) Rabbit anti β actin, by Cell Signaling Technology (1 mentions) Ripa lysis buffer, by Thermo Fisher Scientific (1 mentions) Supersignal west dura kit, by Thermo Fisher Scientific (1 mentions) Nitrocellulose membrane, by Bio-Rad (1 mentions) P mtor ser2448, by Cell Signaling Technology (1 mentions) P perk thr980, by Cell Signaling Technology (1 mentions) Rabbit anti ki67 ab15580, by Abcam (1 mentions) Rabbit anti cyclin d1, by Santa Cruz Biotechnology (1 mentions) Rabbit anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) P eif2α, by Cell Signaling Technology (1 mentions)

3 protocols using rabbit anti murine cox 2

Fatty Acid-Induced Inflammation Study

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Rabbit anti–murine COX-2 (catalog 160106) was from Cayman Chemical; rat anti–mouse F4/80 (catalog ab6640), rabbit anti–VEGF-A (catalog ab52917), anti–IL-1β (catalog ab9722), and anti-Ki67 (catalog ab16667) were from Abcam; rat anti–mouse CD68 (catalog MCA1957B) was from Bio-Rad; rabbit anti-LAMP2A (catalog L0668), mouse anti–β-actin (catalog A1978), and anti–α-smooth muscle actin were from Sigma-Aldrich; mouse anti–IL-6 (catalog sc-32296) and anti–TNF-α (catalog sc-133192) were from Santa Cruz Biotechnology; rabbit anti–p-AKT (Ser473) (catalog 4046, clone D9E), anti-AKT (catalog 4685), anti–perilipin-1 (catalog 9449, clone D1D8), and HMGB1 (catalog 6893S) were from CST; and rabbit anti-CD31/PECAM-1 was from Novus Biologicals (catalog NB100-2284). The HFD was from Bio-Serv (36% fat accounting for 60% of calories, F3282); water-soluble dexamethasone (catalog D2951) and palmitic acid (catalog 506345) were from MilliporeSigma. For the in vitro study, 5 mM palmitate conjugated to 2 mM FFA-free BSA in sterile water (pH 7.4) was used. ONO-4819 (Rivenprost, LS-H93, LSbio) was given at 75 μg/kg/day via Alzet model mini-pump.

Pan Y., Cao S., Tang J., Arroyo J.P., Terker A.S., Wang Y., Niu A., Fan X., Wang S., Zhang Y., Jiang M., Wasserman D.H., Zhang M.Z, & Harris R.C. (2022). Cyclooxygenase-2 in adipose tissue macrophages limits adipose tissue dysfunction in obese mice. The Journal of Clinical Investigation, 132(9), e152391.

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Western Blot Analysis of Cell Lysates

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Whole cell lysates were prepared from SW480-APC and Caco2 cells using RIPA lysis buffer (Pierce Biotechnology) with a proteinase inhibitor cocktail (Calbiochem). Twenty five μg of the lysates were subjected to 10% SDS-PAGE and transferred to nitrocellulose membranes (Bio-Rad). The membrane was blocked with 5% skim milk and was immunoblotted with the primary antibodies overnight at 4°C. Primary antibodies used are rabbit anti-murine Cox-2 (Cayman), rabbit anti-human PPARγ (H-100, Santa Cruz Biotechnology), rabbit anti-β-actin (Cell Signaling). HRP-conjugated donkey anti-rabbit IgG (Promega) was used as secondary antibody. The membrane was exposed on an x-ray film using an enhanced chemiluminescent substrate SuperSignal West Dura Kit (Pierce Biotechnology).

Hyun J., Romero L., Riveron R., Flores C., Kanagavelu S., Chung K.D., Alonso A., Sotolongo J., Ruiz J., Manukyan A., Chun S., Singh G., Salas P., Targan S.R, & Fukata M. (2014). Human Intestinal Epithelial Cells Express Interleukin-10 through Toll-Like Receptor 4-Mediated Epithelial-Macrophage Crosstalk. Journal of Innate Immunity, 7(1), 87-101.

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Antibody Reagents for Protein Analysis

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Affinity-purified rabbit anti-11βHSD2 (catalog no. BHSD22-A) was purchased from Alpha Diagnostic International; rabbit anti-murine COX-2 (catalog no. 160106) was from Cayman Chemicals; rabbit anti-CHOP (catalog no. 2895), rabbit anti-cleaved caspase-3 (catalog no. 9661), rabbit anti-p-ERK (T202/Tyr204, catalog no. 4370), p-mTOR (Ser2448, catalog no 2976), p-PERK (Thr980, Catalog no 3179) and p-eIF2α (Ser51, catalog no 9721) were from Cell Signaling; rabbit anti-cyclin D1 (catalog no. SC-753) was from Santa Cruz Biotechnology; mouse anti-mannose receptor (MR, CD206, catalog no.MAB25341) was from R&D; rabbit anti-Ki67 (ab15580) was from Abcam.

Chang J., Xue M., Yang S., Yao B., Zhang B., Chen X., Pozzi A, & Zhang M.Z. (2015). Inhibition of 11β-Hydroxysteroid Dehydrogenase Type II Suppresses Lung Carcinogenesis by Blocking Tumor COX-2 Expression as Well as the ERK and mTOR Signaling Pathways. PLoS ONE, 10(5), e0127030.

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