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Mitoxantrone hydrochloride

Manufactured by Merck Group
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Mitoxantrone hydrochloride is a synthetic anthracenedione compound used as a laboratory reagent. It is a topoisomerase II inhibitor with cytotoxic properties.

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Lab products found in correlation

Penicillin streptomycin, by Merck Group (2 mentions) Paraffin oil, by Merck Group (1 mentions) Carbon powder, by Merck Group (1 mentions) Zif 8, by Merck Group (1 mentions) Calcium sulfate dihydrate, by Merck Group (1 mentions) Irinotecan hydrochloride, by Merck Group (1 mentions) Activated charcoal, by Merck Group (1 mentions) Trypan blue, by Merck Group (1 mentions) Dulbecco s phosphate buffered saline dpbs, by Merck Group (1 mentions) Fluorescein isothiocyanate fitc dextran, by Merck Group (1 mentions) Rhodamine dextran, by Merck Group (1 mentions) 5 fluorouracil, by Merck Group (1 mentions) Sigmacote, by Merck Group (1 mentions) B16f10 mouse melanoma cancer cells, by American Type Culture Collection (1 mentions) Vascular endothelial growth factor (vegf), by R&D Systems (1 mentions) Chitosan, by Solarbio (1 mentions) Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions) Curcumin, by Merck Group (1 mentions) Platinum 2 acetylacetonate pt acac 2, by Merck Group (1 mentions) Cetyltrimethylammonium bromide, by Merck Group (1 mentions)

6 protocols using mitoxantrone hydrochloride

1

Electrochemical Investigation of Mitoxantrone

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Electrochemical investigation was performed by electrochemical workstation model Ivium-Vertex connected to an electrochemical Cell (Azar electrode Company, Iran). Moreover, the I-V signals were displayed based on the Ag/AgCl/KClsat reference electrode's potential. Mitoxantrone hydrochloride, ZIF-8, BMIMF, and DNA (Calf Thymus) were purchased from Sigma-Aldrich. Also, carbon powder and paraffin oil were obtained from Merck Company. In addition, phosphoric acid, boric acid, acetic acid, and Tris hydrochloride were purchased from Across Company. Notably, the stock solution of Mitoxantrone hydrochloride (0.001 M) was prepared by dissolving 0.517 g Mitoxantrone hydrochloride in 100 mL distillated water under the stirring conditions.
Alizadeh M., Azar P.A., Mozaffari S.A., Karimi-Maleh H, & Tamaddon A.M. (2020). A DNA Based Biosensor Amplified With ZIF-8/Ionic Liquid Composite for Determination of Mitoxantrone Anticancer Drug: An Experimental/Docking Investigation. Frontiers in Chemistry, 8, 814.
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2

Comprehensive Protocol for B16F10 Melanoma Cell Culture and Analysis

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B16F10 mouse melanoma cancer cells were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). Sodium alginate (Protanal LF20/40) of high molecular weight (~250 kDa) was donated by FMC BioPolymers (Philadelphia, PA, USA). Trypan Blue, Dulbecco’s modified eagle’s medium (DMEM), bovine serum albumin (BSA), fetal bovine serum (FBS), penicillin-streptomycin, and trypsin-EDTA solutions, morpholineethanesulfonic acid (MES) hydrate, Dulbecco’s phosphate buffered saline (DPBS), Sigmacote, activated charcoal, calcium sulfate dihydrate, irinotecan hydrochloride, 5-fluorouracil, mitoxantrone hydrochloride, fluorescein isothiocyanate (FITC) dextran (3–6 kDa and 70 kDa), fluorescein isothiocyanate diethylaminoethyl (FITC-DEAE) dextran (3–6 kDa and 70 kDa) and rhodamine dextran (10 kDa) were all purchased from Sigma-Aldrich (St. Louis, MO, USA). Vascular endothelial growth factor (VEGF) and Platelet-derived growth factor (PDGF) proteins and VEGF- and PDGF-DuoSet ELISA kits were purchased from R&D Systems, Inc (Minneapolis, MN, USA). 16-well xCELLigence e-plates were purchased from ACEA Biosciences, Inc. (San Diego, CA, USA).
Emi T., Michaud K., Orton E., Santilli G., Linh C., O’Connell M., Issa F, & Kennedy S. (2019). Ultrasonic Generation of Pulsatile and Sequential Therapeutic Delivery Profiles from Calcium-Crosslinked Alginate Hydrogels. Molecules, 24(6), 1048.
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3

Chitosan-Curcumin Nanoparticle Preparation

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Chitosan (average molecular weight 20 kDa, degree of deacetylation > 90%) was purchased from Solarbio (Beijing, China). Curcumin, methoxy polyethylene glycol (2000 Da) and mitoxantrone hydrochloride were purchased from Sigma-Aldrich (Shanghai, China). 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDCI) and 4-dimethylaminopryidine (DMAP) were purchased from Aladdin Reagent Co. Ltd. (Shanghai, China). Dulbecco’s modified Eagle Medium (DMEM) was purchased from Gibco (Grand Island, NY, USA). Hepg2 cell line was from Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (Shanghai, China).
Chen Y., Wu D., Zhong W., Kuang S., Luo Q., Song L., He L., Feng X, & Tao X. (2018). Evaluation of the PEG Density in the PEGylated Chitosan Nanoparticles as a Drug Carrier for Curcumin and Mitoxantrone. Nanomaterials, 8(7), 486.
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4

Montmorillonite-based Drug Delivery System

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All chemicals were used without purification. Montmorillonite K 10 (MMT) and oleic acid were purchased from Tokyo Kasei (Tokyo, Japan). Cetyltrimethylammonium bromide (CTAB, 99%), mitoxantrone hydrochloride (MIT, 98.0–102.0%), tetraethylene glycol (TEG, C6H14O4), iron(III) acetylacetonate (Fe(acac)3, 99.9+%) and platinum(II) acetylacetonate (Pt(acac)2, 99%) were obtained from Sigma-Aldrich (Saint Louis, MO, U.S.). Ethanol (C2H5OH, 99.8%) was purchased from Nippon Kayaku (Tokyo, Japan). Tetraethylene glycol (C6H14O4) was obtained from Alfa Aesar (Haverhill, MA, USA). The minimum essential medium (MEM), Dulbecco's modified eagle medium (DMEM), fetal bovine serum (FBS), and penicillin-streptomycin-glutamine (PSG) supplement for the culture medium were purchased from Gibco (Waltham, MA, USA).
Chan M.H., Lu C.N., Chung Y.L., Chang Y.C., Li C.H., Chen C.L., Wei D.H, & Hsiao M. (2021). Magnetically guided theranostics: montmorillonite-based iron/platinum nanoparticles for enhancing in situ MRI contrast and hepatocellular carcinoma treatment. Journal of Nanobiotechnology, 19, 308.
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5

Cytotoxicity Evaluation of Mitoxantrone Formulations

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Mitoxantrone hydrochloride, Tween ® 80, Triton TM X-100 (TX-100), Sodium acetate, Phosphate Buffered Saline (PBS), Dulbecco's modified Eagle's medium F-12 (DMEM-F12), penicillin/streptomycin, trypsin, resazurin and paraformaldehyde were acquired from Sigma-Aldrich ® (Sintra, Portugal). Roswell Park Memorial Institute (RPMI-1640) medium was purchased from Gibco (Invitrogen Corporation, Paisley, UK). Cetyl Palmitate was kindly offered by Gatefossé (Nanterre, France). Acetic acid glacial was obtained from VWR International LLC (Radnor PA, USA). Sodium chloride was purchased from Honeywell Fluka TM (NJ, USA). Fetal Bovine Serum (FBS) was purchased from Biochrom AG (Berlin, Germany).
Calcein-AM, Propidium Iodide (PI), cell culture plates and T-flasks were purchased from Thermo Fisher Scientific (Porto, Portugal). Agarose was obtained from Grisp (Porto, Portugal).
Michigan Cancer Foundation-7 (MCF-7) cell line was acquired from ATCC (Middlesex, UK).
Normal Human Dermal Fibroblasts (NHDF) were purchased from Promocell (Heidelberg, Germany). Ultrapure water (18.2 MΩ cm) was obtained from a water purification system (Healforce, Shanghai, China).
Granja A., Lima-Sousa R., Alves C.G., de Melo-Diogo D., Pinheiro M., Sousa C.T., Correia I.J, & Reis S. (2021). Mitoxantrone-loaded lipid nanoparticles for breast cancer therapy - Quality-by-design approach and efficacy assessment in 2D and 3D in vitro cancer models. International journal of pharmaceutics, 607.
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6

Preparation of Cytotoxic Drug Solutions

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Cabazitaxel was obtained from APExBIO (Houston, TX) and dissolved in dimethylsulfoxide (DMSO) to make a 40 mM stock solution. Docetaxel, flavopiridol, mitoxantrone hydrochloride, and piperlongumine were obtained from Sigma Aldrich (St. Louis, MO) and were dissolved in DMSO to make a 45, 160, 400 mM, and 8 mM stock solution, respectively. Trans-resveratrol was obtained from Cayman Chemical (Ann Arbor, MI) and dissolved in DMSO to make a 75 mM stock solution. Alamarblue was obtained from Remel (Lenexa, KS). Propidium iodide was obtained from Sigma Aldrich and dissolved in phosphate-buffered saline for a stock concentration of 50 mg/ml.
Oblad R., Doughty H., Lawson J., Christensen M, & Kenealey J. (2018). Application of Mixture Design Response Surface Methodology for Combination Chemotherapy in PC-3 Human Prostate Cancer Cells. Molecular pharmacology, 94(2).
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