P hsl

Manufactured by Santa Cruz Biotechnology

Sourced in United States

P-HSL is a laboratory product manufactured by Santa Cruz Biotechnology. It is used as a tool for research and scientific investigation. The core function of P-HSL is to serve as a signaling molecule in bacterial quorum sensing systems. No further details or interpretation on its intended use are provided.

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Lab products found in correlation

2 protocols using p hsl

Thermogenic Protein Profiling in BAT and WAT

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20 μg BAT mitochondrial protein or total protein of WAT of WT and ARβ₃KO mice fed with chow diet or HFD (3–5 per group) were size fractionated using 12% SDS-PAGE, transferred to a nitrocellulose membrane and probed with UCP₁ (1:5000 dilution), HSL, p-HSL, AMPk, p-AMPK, ATGL, perilipin and DGAT2 goat-polyclonal IgG primary antibody solution (1:1000 dilution) (Santa Cruz Biotechnology), and β-actin (1:2000 dilution) (ABCAM) was used as a loading control. The secondary antibody solution used was donkey anti-goat IgG conjugate HRP (Santa Cruz Biotechnology), in a 1:2000 dilution.

Preite N.Z., do Nascimento B.P., Muller C.R., Américo A.L., Higa T.S., Evangelista F.S., Lancellotti C.L., Henriques F.D., Batista ML J.r., Bianco A.C, & Ribeiro M.O. (2016). Disruption of beta3 adrenergic receptor increases susceptibility to DIO in mouse. The Journal of endocrinology, 231(3), 259-269.

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Comprehensive Protein Analysis in Adipocytes

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Antibodies against p-AMPK (#P-2535) and AMPK (#2532) were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies against UCP-1 (#Cat sc-293418), PGC-1α (#Cat sc-518038), p-CREB (#Cat sc-81486), CREB (#Cat sc-271), PPAR-γ (#Cat sc-271392), C/EBPα (#Cat sc-166258), p-HSL (#Cat sc-139656), HSL (#Cat sc-74489), FAS (#Cat sc-74540), SIRT-1 (#Cat sc-74540), SREBP-1c (#Cat sc-36553), and β-actin (#Cat sc-47778) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Compound C (CC), H-89, and BAPTA-AM were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Kim D.S., Lee H.Y., Kim H.J., Lee G.H., Lim Y.J., Ko B.M., Kim J.H., Kim T.W., Kim H.K., Kim T.Y., Hwang D.I., Choi H.K., Ju S.M., Chung M.J, & Chae H.J. (2023). Combined Treatment of Mori folium and Mori Cortex Radicis Ameliorate Obesity in Mice via UCP-1 in Brown Adipocytes. Nutrients, 15(17), 3713.

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