The following commercial antibodies were used: goat anti-DMC1 (sc-8973, C-20), goat anti-ATR (sc-1887, N19), mouse anti-SYCP3 (sc-74569, d-1), rat anti-GATA1 (sc-265) antibodies from Santa Cruz. Rabbit anti-H3K4me3 (07–473), from Millipore, rabbit anti-SYCP3 (ab15093) from Abcam, rabbit anti-γH2AX from Trevigen (4411-PC-100) and goat anti-PLZF (AF2944) from R&D systems. Secondary HRP antibodies were purchased from Jackson Laboratories. Fluorescent secondary Alexa antibodies were purchased from Invitrogen.
Rabbit anti h3k4me3 07 473
Manufactured by Merck Group
Sourced in United States
Rabbit anti-H3K4me3 (07–473) is a laboratory reagent used for the detection and quantification of trimethylation of histone H3 at lysine 4 (H3K4me3) in various biological samples. It is a polyclonal antibody raised in rabbits against the H3K4me3 modification. This product can be used in techniques such as Western blotting, immunoprecipitation, and chromatin immunoprecipitation to study epigenetic regulation of gene expression.
Automatically generated - may contain errors
Lab products found in correlation
Goat anti plzf af2944, by R&D Systems (2 mentions)
Rabbit anti sycp3 ab15093, by Abcam (1 mentions)
Hrp secondary antibody, by Jackson ImmunoResearch (1 mentions)
Rabbit anti h3k27me3 07449, by Merck Group (1 mentions)
Rabbit igg, by Merck Group (1 mentions)
Rabbit anti h3k27me3, by Merck Group (1 mentions)
3 protocols using rabbit anti h3k4me3 07 473
1
Immunofluorescence Staining of Meiotic Proteins
2
ChIP-qPCR Analysis of Histone Modifications
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ChIP analysis was performed as previously reported [27 (link)] by using 5 μg of the following antibodies: rabbit anti-H3K4me3 (07473), rabbit anti-H3K27me3 (07449), rabbit anti-H3AcK9 (07352), or rabbit IgG as a negative control (12307) (all from Millipore, MA, USA). 5 ng of immunoprecipitated DNA and the relative control were used as templates for RT-qPCR analysis, performed in duplicate. Primers utilized to amplify the HNF1/6 consensus on HNF4α promoter are as follows: forward 5′-TCCGAAAGACCCAAGTGTGG-3′ and reverse 5′-GCCAATCACGTCCCAGATCA-3′.
3
Antibody Characterization for Cell Research
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The following commercial antibodies were used: rat anti-GATA1 (sc-265), rabbit anti-DMC1 (sc-22768), goat anti-MIS (AMH) (sc-6886) and mouse SYCP3 (sc-74569) were obtained from Santa Cruz; rabbit anti-H3K4me3 (07–473), rabbit anti-H4K5ac (07–327) and rabbit anti-H3K27me3 (07–327) were obtained from Millipore; goat anti-PLZF (AF2944) was obtained from R&D systems; and rabbit anti-SYCP1(ab15087), anti-rabbit DDX4 (ab13840) and anti-rabbit ESR1 (ab32063) were obtained from Abcam. Fluorescent secondary Alexa antibodies were from Invitrogen.
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