Opteia cytokine elisa kits

The following fluorochrome-labeled anti-mouse antibodies were procured from e-Biosciences and BD Biosciences: anti CD3 (145-2C11), anti CD4 (GK 1.5), anti CD8 (53-6.7), anti CD44 (IM7), anti CD62L (MEL-14), anti CD11b (M1/70), anti F4/80 (T45-2342), anti Ly6C (AL-21), anti TNF-α (MP6-XT22), and anti IFN-γ (XMG1.2). Mouse Th17/Treg phenotyping kit (Cat. No. 560767) and IL-1β, IL-4, IL-6, IFN-γ, IL-12, IL-10 BD OptEIA cytokine ELISA kits were procured from BD Biosciences (USA).

Mtb H37Ra was obtained from ATCC (ATCC no. 25177). RPMI 1640 medium, FITC-conjugated anti-human IgG antibody, Lysotracker Red, SYTO 9 and anti-rabbit Alexa Fluor antibody were from Invitrogen. Rabbit anti-iNOS and anti-LAMP-1 antibodies were from Thermo Pierce Biotech. Anti-CD14 mAb was from Boehringer-Mannheim GmbH. Rabbit anti-sheep RBC antibody was from Rockland Immunochemicals. mAbs against Mtb-Acr (IT-4) and LAM (CS-35) were gifted by UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases. Affinity purified peroxidase-conjugated anti-human and anti-mouse IgG antibodies, anti-calmodulin antibody, o-phenylenediamine (OPD), diaminobenzidine (DAB), 2 µM amine-modified green fluorescent polystyrene beads, citrate phosphate dextrose and other laboratory chemicals were from Sigma-Aldrich. BD optEIA cytokine ELISA kits and Middlebrook (MB) media and supplements were obtained from BD Biosciences. Fluorescent NO probe FL2E was purchased from Strem Chemicals, Inc. Ficoll-isopaque was obtained from GE-Biosciences. Ninety-six-well clear glass bottom black imaging plates were from Thermo Nunc.