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Miltenyi cell dissociator

Manufactured by Thermo Fisher Scientific
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The Miltenyi cell dissociator is a laboratory device used to mechanically dissociate tissue samples into single-cell suspensions. It utilizes a combination of controlled shear forces and enzymatic treatment to efficiently break down the extracellular matrix and cell-cell adhesions, enabling the isolation of individual cells from a variety of tissue types.

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Lab products found in correlation

Rpmi 1640 media, by Thermo Fisher Scientific (3 mentions) C tube, by Miltenyi Biotec (3 mentions) Penicillin streptomycin glutamine (psg), by Thermo Fisher Scientific (3 mentions) Liberase, by Roche (3 mentions) Dnase 1, by Roche (3 mentions) Casy cell counter, by Roche (3 mentions) Dnase, by Roche (2 mentions)

3 protocols using miltenyi cell dissociator

1

Tissue Dissociation and Single-Cell Preparation

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Skin and tumour tissues were cut into small 1 mm2 pieces using a scalpel blade and incubated for 2 hr in digestion buffer containing 25 ug/ml liberase (Roche), 250 ug/ml DNAseI (Roche) and 1x DNAse buffer (1.21 Tris base, 0.5 g MgCl2 and 0.073 g CaCl2) at 37°. Following digestion, tissue was transferred into C-tubes (Miltenyi Biotech) containing RPMI-1640 media (Thermo Fisher) supplemented with 10% heat-inactivated foetal calf serum, 1% penicillin-streptomycin-glutamine (Thermo Fisher) and physically disrupted using a Miltenyi cell dissociator. Spleen and LN tissue was disaggregated mechanically using a rubber policeman. All cell suspensions were filtered and cells were counted using a CASY cell counter (Roche).
Hayes M.D., Ward S., Crawford G., Seoane R.C., Jackson W.D., Kipling D., Voehringer D., Dunn-Walters D, & Strid J. (2020). Inflammation-induced IgE promotes epithelial hyperplasia and tumour growth. eLife, 9, e51862.
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2

Tissue Dissociation and Cell Isolation

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Tissue was cut into small 1mm2 pieces using a scalpel blade and incubated for 2 hrs in digestion buffer containing 25ug/ml Liberase (Roche), 250ug/ml DNAseI (Roche) and 1x DNAse buffer (1.21 Tris base, 0.5g MgCl2 and 0.073g CaCl2) at 37°. Following digestion, tissue was transferred into C-tubes (Miltenyi Biotech) containing RPMI-1640 media (Thermo Fisher) supplemented with 10% heat-inactivated foetal calf serum, 1% Penicillin-Streptomycin-Glutamine (Thermo Fisher) and physically disrupted using a Miltenyi cell dissociator. Cell suspensions were then filtered and cells counted using a CASY cell counter (Roche).
Crawford G., Hayes M.D., Castro Seoane R., Ward S., Dalessandri T., Lai C., Healy E., Kipling D., Proby C., Moyes C., Green K., Best K., Haniffa M., Botto M., Dunn-Walters D, & Strid J. (2018). Epithelial damage and tissue γδ T cells promote a unique tumor-protective IgE response. Nature immunology, 19(8), 859-870.
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3

Tissue Dissociation and Cell Isolation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tissue was cut into small 1mm2 pieces using a scalpel blade and incubated for 2 hrs in digestion buffer containing 25ug/ml Liberase (Roche), 250ug/ml DNAseI (Roche) and 1x DNAse buffer (1.21 Tris base, 0.5g MgCl2 and 0.073g CaCl2) at 37°. Following digestion, tissue was transferred into C-tubes (Miltenyi Biotech) containing RPMI-1640 media (Thermo Fisher) supplemented with 10% heat-inactivated foetal calf serum, 1% Penicillin-Streptomycin-Glutamine (Thermo Fisher) and physically disrupted using a Miltenyi cell dissociator. Cell suspensions were then filtered and cells counted using a CASY cell counter (Roche).
Crawford G., Hayes M.D., Castro Seoane R., Ward S., Dalessandri T., Lai C., Healy E., Kipling D., Proby C., Moyes C., Green K., Best K., Haniffa M., Botto M., Dunn-Walters D, & Strid J. (2018). Epithelial damage and tissue γδ T cells promote a unique tumor-protective IgE response. Nature immunology, 19(8), 859-870.
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