Cyclin d1

The blood and dorsal skin samples were collected on the last experimental day. The plasma was separated from the blood samples via centrifugation at 3,000× g and 4 °C for 10 min, and the supernatant was used for further analysis. The levels of IL-10, TGF-β, IL-6, and IL-23 were determined using commercial enzyme-linked immunosorbent assay kits (IL-10, IL-6, and IL-23; MyBiosource, San Diego, CA, USA; TGF-β; Promega, Madison, MI, USA) as per the manufacturer’s instructions. We first rinsed 10 mg of dorsal skin in phosphate-buffered saline to remove excess blood before homogenization. The samples were then homogenized at 1500 × g and 4 °C for 15 min, and the supernatant was collected for assaying. The levels of Ki67, cyclin D1, and neutrophil elastase in the dorsal skin were determined using commercial enzyme-linked immunosorbent assay (ELISA) kits (Ki67; Wuhan Fine Biotech, Hubei, China; cyclin D1; Novus, Centennial, CA, USA; neutrophil elastase; R&D Systems, Inc., Minneapolis, MN, USA) according to the manufacturers’ instructions. The ROS levels in the dorsal skin were determined with an OxiSelect^TM STA-347 in vivo ROS/RNS assay kit (Cell Biolabs, Inc., San Diego, CA, USA) in accordance with the manufacturer’s instructions. Optical density was measured using a microplate reader (Mdecular Devices, Sunnyvale, CA, USA).