VS ribozyme monomer and dimer fractions were passed through 0.2 μm cutoff centrifugal filter units (Millipore) and equilibrated to room temperature. Light scattering measurements were performed with DynaPro spectrometer (Wyatt). Data analysis was performed using native DynaPro software package (Supplementary Fig. 11c, d ).
Dynapro spectrometer
Manufactured by Wyatt Technology
The DynaPro spectrometer is a dynamic light scattering instrument designed to measure the size and size distribution of particles, macromolecules, and other dispersed systems in solution. The DynaPro measures the Brownian motion of particles or molecules, which is directly related to their hydrodynamic size. This information is provided through the calculation of the translational diffusion coefficient and the subsequent determination of the hydrodynamic radius.
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Lab products found in correlation
2 protocols using dynapro spectrometer
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Light Scattering Analysis of Ribozyme
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Light Scattering Analysis of Ribozyme
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VS ribozyme monomer and dimer fractions were passed through 0.2 μm cutoff centrifugal filter units (Millipore) and equilibrated to room temperature. Light scattering measurements were performed with DynaPro spectrometer (Wyatt). Data analysis was performed using native DynaPro software package (Supplementary Fig. 11c, d ).
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