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1

Preparation and Characterization of Vanillin-Benzyl Amine Derivatives

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Ready for dyeing (RFD) bleached cotton fabric (40 × 40/132 × 72 twill weave) of 100 g/cm2 (GSM) and PET (plain weave) fabric of 100 g/cm2 (GSM) having no optical brightening agent added into them were purchased from a local market in New Delhi. Vanillin, benzyl amine, 2,2-diphenyl-1-picrylhydrazyl (DPPH), sodium borohydride, methanol, and ethanol were purchased from Central Drug House (CDH) and used in the study without further purification. Analytical grade TLC Silica gel 60 F254 plate and extra pure Agar–Agar were purchased from Merck and Luria broth was purchased from Himedia, respectively which were used in the antibacterial study. CDCl3, Deuterated DMSO and KBr of analytical grade were purchased from Sigma Aldrich and used for NMR and FTIR sample preparation, respectively.
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2

Babchi Oil-based Pharmaceutical Formulation

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Babchi oil was acquired from Pukhraj Herbals, Mandsaur (India). Ethyl cellulose, ethanol and acetone were purchased from Central Drug House, New Delhi. Dichloromethane and polyvinyl alcohol were procured from SD Fine Chemicals Limited, Mumbai. Potassium dihydrogen phosphate and sodium hydroxide were supplied by HPLC, Mumbai. Nutrient agar was purchased from Hi-media, India. All the other chemicals and solvents used were of analytical grade. Distilled deionized water was used throughout this work.
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3

Development of α-MNG Nanoformulation

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α-MNG (MW 410.46 g/mol; purity = 98%) was obtained from Sigma-Aldrich. PLGA [poly(D, L-lactide-co-glycolide)] lactide: glycolide (50:50) (Mw = 44,628 g/mol; viscosity, 0.71–1.0 dl/g) (Merck, India). Polyvinyl alcohol (PVA) was obtained from Sisco Laboratory (Mumbai, India) and Rhodamine B (RhB) from Sigma-Aldrich (St. Louis, MO, USA). The laboratory solvents, namely acetone, dichloromethane, acetonitrile, and deionized water, were procured from SD Fine Chemicals (Mumbai, India). LC-MS grade reagents, namely acetonitrile, methanol, ammonium acetate, ethyl acetate, glacial acetic acid, and water, were purchased from Sigma-Aldrich (St. Louis, MO, USA). The analytical grades of buffer reagents, i.e., phosphate-buffered saline (PBS), sodium dihydrogen phosphate, sodium hydroxide, disodium hydrogen phosphate, potassium dihydrogen phosphate, and ethanol, were procured from Central Drug House (New Delhi, India). For HPLC analysis, double distilled water was filtered through a micron-sized membrane filter (pore size, 0.45µ) and membrane filter (Durapore pore size, 0.21 µ) using an injectable needle.
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4

Synthesis of Pyridine-based Metal Complexes

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All reagents and chemicals are of analytical grade and have been used as received without further purification. 2,6-pyridinedicarboxylic acid was purchased from Sigma Aldrich, Germany, CoCl2•2H2O was supplied by Alfa Aesar, UK. Solvents: Ethanol (98.8%), N,Ndimethyl formamide (99.0%), mEthanol (99.9%), dichloromethane (99.8%), acetone (99.9%) and chloroform (99.5%) were obtained from Central Drug House (P) Ltd New Delhi India.
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5

Characterization of Pharmaceutical-Grade MA Formulation

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MA (purity > 99.5%) was received as a kind gift sample from Orchid Pharmaceutical Ltd. (Roorkee, India). Hydroxypropyl methylcellulose-K4M (HPMC-K4M), sodium dodecyl sulfate (SLS), polysorbate 80 (Tween ® 80), hydrochloric acid (HCl), methanol (MeOH), dimethyl sulfoxide (DMSO), sodium hydroxide (NaOH), potassium dihydrogen phosphate (KH 2 PO 4 ), sodium chloride (NaCl), ethanol (C 2 H 5 OH), potassium chloride (KCl), disodium hydrogen phosphate dodecahydrate (Na 2 HPO 4 .12H 2 O), N,N-dimethylformamide (DMF), and acetonitrile (CH 3 CN) were commercially obtained from Central Drug House Ltd. (New Delhi, India). The water utilized for all experiments was high-purity/deionized water, which was obtained using a Milli-Q ® water purification unit (Milli-Q Co., Lyon, France). All other materials used were of analytical/pharmaceutical grade.
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