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Anti cd43

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Sourced in Germany, United States

Anti-CD43 is a monoclonal antibody that binds to the CD43 antigen expressed on the surface of T cells, B cells, and monocytes. CD43 is a sialoglycoprotein that plays a role in cell adhesion and signal transduction.

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Lab products found in correlation

Anti cd45, by BioLegend (2 mentions) Anti cd8, by Thermo Fisher Scientific (1 mentions) Live dead dye, by Thermo Fisher Scientific (1 mentions) Anti cd3, by Miltenyi Biotec (1 mentions) Lsrfortessa, by BD (1 mentions) Anti cd4, by BD (1 mentions) Trucount tube, by BD (1 mentions) Anti cd38, by BD (1 mentions) Anti cd34, by Thermo Fisher Scientific (1 mentions) Anti cd33, by BioLegend (1 mentions) Methocult h4434 classic, by STEMCELL (1 mentions) Anti cd45, by BD (1 mentions) Anti cd8, by BD (1 mentions) Anti cd21, by BioLegend (1 mentions) H57 597, by Thermo Fisher Scientific (1 mentions) Facsvantage cell sorter, by BD (1 mentions) Streptavidin rpe, by Agilent Technologies (1 mentions) Mb19 1, by BioLegend (1 mentions) Anti tcrb, by Thermo Fisher Scientific (1 mentions) Anti cd4, by Thermo Fisher Scientific (1 mentions)

4 protocols using anti cd43

1

Quantifying Immune Cell Populations in Tissue Grafts

Cited 1 time
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Flow cytometry was used to quantify the distribution of different DL cell types in both the graft and perfusate compartments as previously described25 (link) (Table 1). Isolated cells were washed and stained with Live/Dead dye (eBioscience) in PBS and then stained with the following antibodies: anti-CD45 (Biolegend), anti-CD3 (Miltenyi Biotec), anti-CD4 (BD Bioscience), anti-CD8 (eBioscience), anti-CD43 (Biolegend), anti-His48 (eBioscience), anti-CD161 (Biolegend), anti-CD45R (eBioscience). Cell counts (cells/mL) were determined using BD Trucount tubes. Flow cytometric compensation was performed using fluorescent compensation beads, and cells were analyzed using a multiparameter flow cytometer (LSRFortessa BD Biosciences). Analysis of flow results was performed with FlowJo Software (Version 10; FlowJo LLC, Ashland, OR).
Davis R.P., Yerxa J., Gao Q., Gloria J., Scheuermann U., Song M., Zhang M., Parker W., Lee J., Hartwig M.G, & Barbas A.S. (2020). Donor Leukocyte Trafficking and Damage-associated Molecular Pattern Expression During Ex Vivo Lung Perfusion. Transplantation Direct, 6(3), e532.
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2

Hematopoietic Differentiation of iPSCs

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1 × 106 healthy donor or GS-2 iPSCs were co-cultured with Op9 stromal cells (kindly provided by Prof. Dr. Juan Carlos Zúñiga-Pflücker, Sunnybrook Research Institute, Toronto, Canada [20 (link)]) in different HSC differentiation media for 8 days. A total of 7 clones (2 from healthy donor iPSCs, 5 derived from 3 different GS-2 patients) were tested for HSC differentiation potential. HSC differentiation media consisted of serum-free StemMACS HSC expansion medium with 1× hematopoietic expansion cocktail (STF: SCF, TPO, Flt3-ligand), 50 ng/mL BMP4 (Bone Morphogenic Protein 4 (R&D Systems, 314-BP-050), 5 μM ATRA (all-trans retinoic acid, Sigma-Aldrich, R2625) and 1 μM dexamethasone. Differentiated CD34+ HSC were further expanded with StemMACS HSC expansion medium with 1× STF for 1–2 weeks. Immunophenotype of the differentiated cells was measured using anti-CD43 (BioLegend, 343206), anti-CD45 (BD Biosciences, 560976), anti-CD34 (eBioscience, 17-0349-42), and anti-CD38 (BD Biosciences, 555459) antibodies. To assess the colony-forming capacity of HSCs differentiated from iPSC, the cells were cultured in Methocult H4434 classic (Stem Cell Technologies, 04444) for 10–14 days. After counting, colonies were picked and stained with anti-CD45, anti-CD16 (BioLegend, 302012), anti-CD14 (BD Biosciences, 555399), and anti-CD33 (BioLegend, 366608) to confirm myeloid differentiation.
Güney-Esken G., Erol Ö.D., Pervin B., Gürhan Sevinç G., Önder T., Bilgiç E., Korkusuz P., Günel-Özcan A., Uçkan-Çetinkaya D, & Aerts-Kaya F. (2021). Development, characterization, and hematopoietic differentiation of Griscelli syndrome type 2 induced pluripotent stem cells. Stem Cell Research & Therapy, 12, 287.
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3

Immunophenotyping of B and T Cells

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Tumors were analyzed by flow cytometry using the following cell surface markers for (a) B cells: RA3-6B2, anti-B220; R2/60, anti-CD43; II/41, anti-IgM; 11/26C, anti-IgD; MB19-1, anti-CD19; 53-7.3, anti-CD5 and B3B4, anti-CD23; 7E9, anti-CD21 (BioLegend, Fell, Germany); and (b) T cells: GK1.5, anti-CD4; H57-597, anti-TCRb (all from eBioscience, Vienna, Austria) and 53-6.7, anti-CD8; (from BD Pharmingen, San Diego, CA, USA). Biotinylated antibodies were monitored with streptavidin-RPE (DAKO, Vienna, Austria) or streptavidin-PE-Cy7 (BD Phamingen). Using a FACSVantage cell sorter (Becton Dickinson, Heidelberg, Germany) premalign pre/pro-B cells (B220+/IgM−) or immature (IgM+/IgDlow) and mature (IgM+/IgDhigh) B cells were isolated and sorted from bone marrow and spleen, respectively. Flow cytometry data were analyzed using Cyflogic free ware and FlowJo (Ashland, OR, USA).
Peintner L., Dorstyn L., Kumar S., Aneichyk T., Villunger A, & Manzl C. (2015). The tumor-modulatory effects of Caspase-2 and Pidd1 do not require the scaffold protein Raidd. Cell Death and Differentiation, 22(11), 1803-1811.
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4

Temporal Dynamics of Stem Cell Markers

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Blood samples (0.5 mL) were drawn from the tail vein before tMCAO (basal sample) and at 1 h, and at 3, 7, 14, and 28 days after tMCAO. The samples were collected into K2EDTA tubes (BD Microtainer, Franklin Lakes, NJ, USA). Immunofluorescence cell staining was performed with the fluorescent conjugated antibodies anti-CD34 (ref.: sc-7324, Santa Cruz Biotechnology, Dallas, TX, USA), anti-ckit (ref.: sc-19619, Santa Cruz Biotechnology, Dallas, TX, USA), anti-KDR (ref.: bs-10412R, Bioss, Woburn, MA, USA), and anti-CD43 (ref.: 202814, Biolegend, San Diego, CA, USA). Cell fluorescence was measured 15 min after staining by flow cytometry with BD FACS Aria II (BD, Bioscience, Franklin Lakes, NJ, USA). The numbers of each cellular lineage were calculated using the FACSDiva software (https://www.bdbiosciences.com/en-us/products/software/instrument-software/bd-facsdiva-software, accessed on 18 January 2024; BD Biosciences, Franklin Lakes, NJ, USA).
Romaus-Sanjurjo D., Castañón-Apilánez M., López-Arias E., Custodia A., Martin-Martín C., Ouro A., López-Cancio E, & Sobrino T. (2024). Neuroprotection Afforded by an Enriched Mediterranean-like Diet Is Modified by Exercise in a Rat Male Model of Cerebral Ischemia. Antioxidants, 13(2), 138.
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